• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  6. 6
  7. 7
  8. 8
  9. 9
  10. 10
  11. 11

Investigate the effect of changing conditions on the rate of reaction between Catalase (Celery) acid and Hydrogen Peroxide (H2O2).

Extracts from this document...


Aim: To investigate the effect of changing conditions on the rate of reaction between Catalase (Celery) acid and Hydrogen Peroxide (H2O2). Method: I will first set up the experiment as shown in the diagram. To make it safe I will keep everything tidy and then get out the safety equipment (goggles, apron). Then I will get the chemicals prepared in the right measurements, so I will not have to do that every time. To get catalase you have to use a pestle and mortar to grind up the celery with water to make a solution. Also I will prepare one large amount of the enzyme from the celery to make sure it is all the same. As soon as I mix the catalase and H202 I will put the cork connected to the delivery tube in the boiling tube and start the timer. I will make observations and after 50-70 seconds. I will squeeze the tube to prevent any more gas getting through as I record the results. As I complete each experiment I will plot a graph of the results. Variables: The variables are: Temperature Concentration (catalase) Concentration (H2O2) pH of solution These will all affect the experiment and results. The temperature will affect the results because the higher the temperature the more energy the reaction has. ...read more.


= repeat Analysis: To display the information in a way I could analyse the results I put them into a graph. A graph to show the relationship between the Temperature and amount of oxygen produced = Line of best fit The optimal temperature for this enzyme ---------------- = Expected pattern is somewhere between 50 and 60oC Finding that the results were quite accurate I went on to make a graph of rates to help find a pattern. I found the rates by using the formula: Variable Amount of Gas Rate = ------------ = -------------------- Time 20 Seconds First I made A Table of Rates: Temperature (oC) Time (Seconds) Average amount of oxygen (ml) Rate (cm3 per second) 10 60 9.33 0.1555 20 60 10 0.166 30 60 12.33 0.2055 40 60 14.66 0.24433 50 60 22 0.366 60 60 2.66 0.04433 The word and chemical equations for this reaction are: Catalase + Hydrogen Peroxide Oxygen + Water Catalase + H2O2 O2 + H2O A graph to show the relationship between the Temperature and rate of reaction ---------------- = Line of best fit This graph clearly shows a bell shaped curve with a sharp fall, my prediction was therefore correct. In the 10-50 degree scale, as the temperature increases the oxygen produced increases and therefore the rate of reaction increases. ...read more.


This is accurate to a point but could be better with a thermostatically controlled water bath, where the temperature can just be set. This would also allow for a much larger range of readings. The measuring cylinder could be replaced by a gas syringe, which can measure to a better degree of accuracy giving better readings. Also the test could be repeated several more times with a wider or more concise range of readings taken. This would produce more accurate results and the more points on a graph you have the more accurate you can be. For additional information you could extend the test further by testing to see what happens to the rate of reaction after 60 seconds. This experiment could be applied to the human body. Hydrogen Peroxide is a poison to the body and needs removing. For this reason the liver produces catalase to break it down. Therefore the experiment could have been conducted using ground up liver. It could also be applied to other enzymes in plants to find the ideal growing temperatures that could lead to increased or decreased production. An extension could be made on the experiment by investigating what effect some of the other variables have on the reaction, such as concentration and pH. With these results I could form an ideal condition for the enzyme catalase in celery. Kieran Wood Catalase and Hydrogen Peroxide 27.11.2000 Mr Burns Experiment ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The Decomposition of H2O2 using Catalase, in yeast as a catalyst.

    Volume O2 given off (cm3) 0.15 4.0 0.30 6.0 0.45 7.0 1.00 8.0 1.15 8.5 1.30 9.0 1.45 9.5 2.00 10.0 2.15 11.5 2.30 12.0 2.45 12.0 3.00 13.0 3.15 13.0 3.30 13.5 3.45 14.0 4.00 14.0 From this test I wanted to find out how long it would take for the volume of H2O2 I used to completely decompose.


    as the concentration of bile salt increases the average fall of pH decreases, the figure maintained for 2% is therefore an anomaly, reasons for this will be discussed in evaluation. When the concentration of bile salt was 3% the average fall increased to 2.2, this increase continued in concentration of

  1. Effect Of Substrate Concentration On The Activity Of Catalase

    The 50cm3 measuring cylinder used to measure the volume of hydrogen peroxide was ideal as the amount of hydrogen peroxide was wasn't too little that it would be inappropriate and also inaccurate to measure with a 50cm3 measuring cylinder, nor was it so large, that it would just about be measured.

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Precision: Before beginning the experiment make sure that all data is measured in specific units as to limit the chances of confusion or the recording of imprecise data. This means that one would need to measure (using cylinders and burettes, etc.)

  1. The effect of aspirin on the action of bovine liver catalase

    can be measured to the nearest 0.5mm This gives an error of � 0.8 mm� for the volume of oxygen produced. Approximately 60mm� of oxygen is produced this gives a percentage error of around 1.3 %. This is very accurate.

  2. To determine the rate law for a chemical reaction among hydrogen peroxide, iodide and ...

    Ans: Since 2S2O32-(aq) + I2(aq) --> 2I-(aq) + S4O62-(aq) The average titre of S2O32- is 34.43cm3 The no. of mole of S2O32- = 1/2 no. of mol of I2 The no. of mole of S2O32- = 0.05 mol dm-3 x (34.43/1000)

  1. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    Use the same concentration of catalase throughout the experiment. This is done by using 10 (1 mm) potato discs in each experiment. Use the same volume of catalase for each concentration of hydrogen peroxide. Reaction time (Controlled) The time will affect the amount of product produced. This is because the more time given to an experiment, the greater the amount of product produced.

  2. Investigating how varying the concentration of substrate (hydrogen peroxide) affects the rate of reaction ...

    Also as a risk assessment I have looked at the caution card for hydrogen peroxide. * Interpretation From my results I have plotted a graph to show the amount of oxygen given off at each concentration of hydrogen peroxide. I have plotted all five concentrations on the same graph in order to compare the rate of reaction at each concentration.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work