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# Investigate the effect of changing conditions on the rate of reaction between Catalase (Celery) acid and Hydrogen Peroxide (H2O2).

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Introduction

Aim: To investigate the effect of changing conditions on the rate of reaction between Catalase (Celery) acid and Hydrogen Peroxide (H2O2). Method: I will first set up the experiment as shown in the diagram. To make it safe I will keep everything tidy and then get out the safety equipment (goggles, apron). Then I will get the chemicals prepared in the right measurements, so I will not have to do that every time. To get catalase you have to use a pestle and mortar to grind up the celery with water to make a solution. Also I will prepare one large amount of the enzyme from the celery to make sure it is all the same. As soon as I mix the catalase and H202 I will put the cork connected to the delivery tube in the boiling tube and start the timer. I will make observations and after 50-70 seconds. I will squeeze the tube to prevent any more gas getting through as I record the results. As I complete each experiment I will plot a graph of the results. Variables: The variables are: Temperature Concentration (catalase) Concentration (H2O2) pH of solution These will all affect the experiment and results. The temperature will affect the results because the higher the temperature the more energy the reaction has. ...read more.

Middle

= repeat Analysis: To display the information in a way I could analyse the results I put them into a graph. A graph to show the relationship between the Temperature and amount of oxygen produced = Line of best fit The optimal temperature for this enzyme ---------------- = Expected pattern is somewhere between 50 and 60oC Finding that the results were quite accurate I went on to make a graph of rates to help find a pattern. I found the rates by using the formula: Variable Amount of Gas Rate = ------------ = -------------------- Time 20 Seconds First I made A Table of Rates: Temperature (oC) Time (Seconds) Average amount of oxygen (ml) Rate (cm3 per second) 10 60 9.33 0.1555 20 60 10 0.166 30 60 12.33 0.2055 40 60 14.66 0.24433 50 60 22 0.366 60 60 2.66 0.04433 The word and chemical equations for this reaction are: Catalase + Hydrogen Peroxide Oxygen + Water Catalase + H2O2 O2 + H2O A graph to show the relationship between the Temperature and rate of reaction ---------------- = Line of best fit This graph clearly shows a bell shaped curve with a sharp fall, my prediction was therefore correct. In the 10-50 degree scale, as the temperature increases the oxygen produced increases and therefore the rate of reaction increases. ...read more.

Conclusion

This is accurate to a point but could be better with a thermostatically controlled water bath, where the temperature can just be set. This would also allow for a much larger range of readings. The measuring cylinder could be replaced by a gas syringe, which can measure to a better degree of accuracy giving better readings. Also the test could be repeated several more times with a wider or more concise range of readings taken. This would produce more accurate results and the more points on a graph you have the more accurate you can be. For additional information you could extend the test further by testing to see what happens to the rate of reaction after 60 seconds. This experiment could be applied to the human body. Hydrogen Peroxide is a poison to the body and needs removing. For this reason the liver produces catalase to break it down. Therefore the experiment could have been conducted using ground up liver. It could also be applied to other enzymes in plants to find the ideal growing temperatures that could lead to increased or decreased production. An extension could be made on the experiment by investigating what effect some of the other variables have on the reaction, such as concentration and pH. With these results I could form an ideal condition for the enzyme catalase in celery. Kieran Wood Catalase and Hydrogen Peroxide 27.11.2000 Mr Burns Experiment ...read more.

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