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Investigate the effect of substrate concentration on an enzyme reaction rate, how it varies at different concentration solutions.

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Introduction

Enzyme Reaction Rates Aim: To investigate the effect of substrate concentration on an enzyme reaction rate, how it varies at different concentration solutions Background Information: * Enzymes such as Catalase are protein molecules, which are found in living cells. They are used to speed up specific reaction within the cell. They are all very specific as each enzyme just performs one particular reaction. * Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen. * It is able to speed up the decomposition of Hydrogen peroxide because the shape of its active site matches the shape of the Hydrogen peroxide molecule. This type of reaction where a molecule is broken down into smaller pieces is called an Anabolic Reaction. Prediction: From my own knowledge about this experiment I predict that if catalase enzymes in the potatoes and the temperature are kept constant at all time through the experiment, the ...read more.

Middle

the first step is to create your required concentration by mixing the certain about of distilled water with HP solution (2molar) in the beaker, from the table we see that 40ml is produced which is more than required, the reason is to be able to use the same solution created 3 times 4. then 10ml of this solution is measured in a measuring cylinder and then is purred into the boiling tube 5. the test tube is filled with bathwater 6. 10 pieces of potato chips (2mm thick) are cut accurately and they are purred into the boiling tube containing the hydrogen peroxide solution 7. immediately the digital timer is started and we shake the boiling tube and wait for 2 minutes before starting to could oxygen bubbles 8. after 2 minutes the clock is restarted, the delivery tube connects the boiling tube to the test tube and then we start to count the oxygen bubbles per minute 9. the same procedure is carried out 3 times for each concentration Results: Concentration of HP (molar) ...read more.

Conclusion

much more accurate and keep measurements constant but it may differ each time that I measured a solution in a measuring cylinder * The diameter of the potato chips were always kept constant and accurate through out the experiment but their length which was suppose to be 2mm per piece may have not been very accurate because I had to cut each piece using eye accuracy and ruler which as you can see is not very practical and accurate, different potato chip sizes have different surface area and therefore affect the rate of reaction * Each time I have had to start and stop the digital time manually and I may have taken to long each time and therefore it decreases the accuracy of results due to wrong timing * I have an incorrect result in my result table and graph, this may have been due to using the wrong concentration solution or wrong size potato chips because these are the main factors which can alter the result in such a way Amin Shahiri 12AW Biology AS Enzyme Concentration Assignment 1 ...read more.

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