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Investigate the effect of sucrose concentration on osmosis in a potato chip.

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Osmosis Investigation Aim: - To investigate the effect of sucrose concentration on osmosis in a potato chip. Introduction: - Osmosis is the diffusion of water from an area of high water concentration to an area of low water concentration through a semi- permeable membrane of freely moving water molecules. Osmosis is the movement through a special membrane. The rate of which osmosis takes place is effected by concentration of water on each side of the semi-permeable membrane. For example, if there was more water on the left side than right, the net amount of water would travel to the right side through the process of osmosis. Osmosis occurs in the roots of plants, in our body e.g. the large intestine where excess water is absorbed. Back-round Information: - Osmosis occurs in roots, as there is a higher concentration of water in the soil rather than the roots. The cell walls act as a semi- permeable membrane. Water in the plant is extremely important as it keeps the cells turgid, a lot of water is also lost by transpiration and water is also needed in the process of photosynthesis: Carbon Dioxide + Water Glucose + Oxygen Plant cells have two semi- permeable membranes to allow water to enter the vacuole. The membranes called the plasmalemma which is lining the inside of the cell wall; and the tonoplast which is lining the vacuole are semi-permeable. ...read more.


* Use the borer to cut out potato cylinders. * Cut them into twelve pieces. * Weigh the cut potato chips on the accurate balance and record the results on a table. * Measure 25cm3 of all the solutions of sugar (0.0, 0.2, 0.4, 0.6, 0.8, 1.0 Molar) and place them into the petri dishes. * Place two potato chips in each petri dish. * Leave all the petri dishes containing the potato chips and solutions in the same place for three days. * After three days measure and weigh the potato chips. * Record the results. * Repeat this process twice for accurate results. Safety: To carry out this experiment safely I will not pour the solutions from a high height and use the cork borer and knife safely keeping them away from the edge of the table. The table my results will be recorded on will look similar to this. Results:- Sucrose/water Solutions (mol/litre) Mass (g) Amount of solution (ml) 0.0 25 0.2 25 0.4 25 0.6 25 0.8 25 1.0 25 Table 1: Initial Sucrose/water Solutions (mol/litre) Mass (g) Amount of solution (ml) 0.0 P1: 1.47 P2: 1.23 25 0.2 P1: 1.17 P2: 1.36 25 0.4 P1: 1.14 P2: 1.54 25 0.6 P1: 1.22 P2: 1.49 25 0.8 P1: 1.40 P2: 1.19 25 1.0 P1: 1.34 P2: 1.15 25 Table 2: After Sucrose/water Solutions (mol/litre) Mass (g) 0.0 P1: 1.78 P2: 2.07 0.2 P1: 1.29 P2: 1.07 0.4 P1: 1.35 P2: 1.03 0.6 P1: 0.93 P2: 1.12 0.8 P1: 0.88 P2: 1.07 1.0 P1: 0.96 P2: 0.83 Table 3: Percentage change in mass: Sucrose Solution (Mols) ...read more.


solutions for a longer time period to allow the solution to penetrate fully, carrying out the experiment with a greater number of concentrations and repeating it more times for each concentration of sugar solutions. The rest of the points on my graph are close to a curve line. Since most of my results were fairly accurate I can say with reasonable confidence that my results were valid. For further work to provide additional evidence I could repeat this experiment by using a greater range of solution strengths or using tissue samples from other plants to discover whether my prediction is true of other plant tissues and not only correct for potatoes. Also I could calculate the rate of diffusion by Fick's law as it is used to measure the rate of diffusion. It states that: (The symbol a means 'proportional to') The larger the area and difference in concentration and the thinner the surface, the quicker the rate. Which could have given me a more precise result. I would also take into account all the changes I have mentioned for my method e.g. keeping the petri dishes in a water bath at a set temperature, by keeping them at a constant pressure, leaving the potato chips in the solutions for a longer time period to allow the solution to penetrate fully, carrying out the experiment with a greater number of concentrations and repeating it more times for each concentration of sugar solutions ?? ?? ?? ?? Reena Gandesha 12.1 Biology Investigation ...read more.

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