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Investigate the effect of temperature on amylase activity.

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Introduction

Biology coursework Effect of temp. on amylase activity *Aim: investigate the effect of temperature on amylase activity *Prediction: i think that as the temperature is increased the reaction rate will also increase. I think this because when the temperature rises the molecules move faster and are more likely to react. *method: 1. fill spotting tile with iodine 2. fill one test tube with starch and amylase 3. start stop clock 4. ...read more.

Middle

use the same amount of starch and amylase each time. 2. use same amount of iodine each time. The only thing I will change is the temperature *safety: to make sure the experiment is carried out in the safect possible way; long hair is to be tied back, goggles must be worn, bags must be under the tables, and caution taked with any hot water or solutions. *results: Temp (�C) ...read more.

Conclusion

My results were strong enough to give me a good clnclusion and they had no anomalies. I noticed a pattern in my graph which shows that the reaction rate increases as the temperature does, until 40 C and then the rate decreases as the enzymes become denatured. My conclusion supports my prediction that the best temperature would be 40 and it would denature a 60-70 C. there are other experiments that I could do to give me better results, these are: investigation of varied concentrations of starch and/or amylase ...read more.

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