Investigate the effect of temperature on amylase activity.

2. fill one test tube with starch and amylase

3. start stop clock

4. put 3 drops of starch and amylase into spotting tile wells

5. repeat till no colour change

6. record time

7. repeat

*apparatus:    - spotting tile

- test tube

- stop clock

- iodine

- starch

- amylase

- measuring cylinder

- water bath

*range of readings: I will take readings at 3˚C - 80˚C every 3o seconds until there is no more colour change

*fair test: my experiment must be a fair test and I will make sure it is by: 1. use the same amount of starch and amylase each time. 2. use same amount of iodine each time. The only thing I will change is the temperature

*safety: to make sure the experiment is carried out in the safect possible way; long hair is to be tied back, goggles must be worn, bags must be under the tables, and caution taked with any hot water or solutions.

*results:

Conclusion: the amylase worked best at 40 C after this the reaction slowed down as the enzymes became de-natured.  My results were strong enough to give me a good clnclusion and they had no anomalies.  I noticed a pattern in my graph which shows that the reaction rate increases as the temperature does, until 40 C and then the rate decreases as the enzymes become denatured.  My conclusion supports my prediction that the best temperature would be 40 and it would denature a 60-70 C.  there are other experiments that I could do to give me better results, these are: investigation of varied concentrations of starch and/or amylase