• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigate the effect of temperature on amylase activity.

Extracts from this document...

Introduction

Biology coursework Effect of temp. on amylase activity *Aim: investigate the effect of temperature on amylase activity *Prediction: i think that as the temperature is increased the reaction rate will also increase. I think this because when the temperature rises the molecules move faster and are more likely to react. *method: 1. fill spotting tile with iodine 2. fill one test tube with starch and amylase 3. start stop clock 4. ...read more.

Middle

use the same amount of starch and amylase each time. 2. use same amount of iodine each time. The only thing I will change is the temperature *safety: to make sure the experiment is carried out in the safect possible way; long hair is to be tied back, goggles must be worn, bags must be under the tables, and caution taked with any hot water or solutions. *results: Temp (�C) ...read more.

Conclusion

My results were strong enough to give me a good clnclusion and they had no anomalies. I noticed a pattern in my graph which shows that the reaction rate increases as the temperature does, until 40 C and then the rate decreases as the enzymes become denatured. My conclusion supports my prediction that the best temperature would be 40 and it would denature a 60-70 C. there are other experiments that I could do to give me better results, these are: investigation of varied concentrations of starch and/or amylase ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Rates of Reaction - The Iodine Clock

    A rise in temperature will have the effect of increasing the rate of reaction since the particles within the solution are given extra energy - the particles will have more collisions and since they have more energy they will have more chance of reacting.

  2. The Iodine Clock Investigation

    the concentration of H2O2 has an effect on the rate of the reaction 2. in the main experiment, the concentration of H2O2 should perhaps be reduced, as the time taken for the reaction to reach an end point was relatively short.

  1. The Iodine Clock

    had ended or solution had changed colour, even though we kept the person the same each time I still do not think that this would be the most accurate way. I made sure that I kept all the other factors that could have affected the experiment the same throughout the

  2. Investigating the effect of enzyme concentration on the hydrolysis of starch with amylase.

    Every enzyme has an optimum pH, which is the best condition for the enzyme to breakdown the starch fastest. I f the pH level is too strong the enzyme will be denatured and effect the rate of reaction by reducing it.

  1. Activity of Diastase On Starch

    ml Mean volume of reaction mixture used = 8.6+8.6+8.5 = 8.57 ml 3 Effect of change in temperature on the rate of hydrolysis of starch. Temperature Volume of reaction mixture used Rate= 1/volume of reaction mixture used 20�c 6.44 ml 0.155 30�c 5.57 ml 0.180 40�c 6.23 ml 0.161 70�c

  2. The effect of concentration on the activity of catalase.

    Care will be taken to form the cubes of potatoes so that they have accurate volumes and will produce reliable results. For precision, a ruler will be used to form the potato cubes. The recordings of the volume of oxygen produced will be taken at eye level to the measuring cylinder to obtain accurate readings.

  1. Catalase activity in the liver

    But it is able for the liver to detoxify the compound using the enzyme catalase. What the enzyme catalase does in this experiment is to convert hazardous hydrogen peroxide into harmless oxygen and water. This is what takes place in the reaction - 2H202 2H20 + 02 The enzyme catalase

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    This due to the fact that after 40�C, the optimum temperature for catalase enzyme activity, the weak bonds that hold together the enzyme structure, start to break (this is especially true of hydrogen bonds), due to the increased kinetic energy.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work