• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
  12. 12
    12

Investigate the effect of temperature on the rate of reaction for the enzyme amylase.

Extracts from this document...

Introduction

Planning Introduction Enzymes are found in the body and are used for the digestion of different food molecules. Amylase breaks down Starch to glucose while other enzymes break down other food molecules. Aim I am experimenting to investigate the effect of temperature on the rate of reaction for the enzyme amylase. Background Knowledge Some enzymes function best at 37oC, which is body temperature. This is a proven theory for the enzyme amylase. If the temperature is above or below 37oC these enzymes function slower, and therefore take more time to break down these food molecules. However, at very high temperatures enzymes change shape and no longer carry out their function, they are said to be denatured. This is why I am testing to see how temperature affects the rate of reaction in detail. Amylase is also known as carbohydrase as used in the diagram below. (Diagram from http://www.gcsescience.com) Variables Amount of Glucose Present. This is the dependent variable of my experiment I will test this using iodine to display if there is glucose present. I will test this after the amylase has had enough time to break down the starch. If the iodine should change black in the final solution, it means no reaction has taken place, but should it turn orange, a reaction has taken place. ...read more.

Middle

5 minutes 50oC 6 minutes 55oC 10 minutes 60oC No Reaction From the trial data it is clear to see the only variables that have an effect on the experiment are time and temperature. Obtaining Evidence Introduction These are my results I found out when I did my experiment. I repeated the experiment 3 times to ensure the best accuracy. Experiment 1 Temperature Time 25�C 05:00 30�C 03:00 35�C 01:30 40�C 02:00 45�C 03:00 50�C 03:40 55�C 04:00 60�C No Reaction Experiment 2 Temperature Time 25�C 02:00 30�C 01:45 35�C 01:20 40�C 02:00 45�C 02:40 50�C 03:00 55�C 03:20 60�C No Reaction Experiment 3 Temperature Time 25�C 03:30 30�C 02:40 35�C 01:00 40�C 02:00 45�C 03:20 50�C 04:00 55�C 04:40 60�C 05:20 Mean Averages Temperature Time 25�C 210 Seconds 30�C 165 Seconds 35�C 76 Seconds 40�C 120 Seconds 45�C 180 Seconds 50�C 213 Seconds 55�C 240 Seconds 60�C N/A Analysis Experiment 1 In this graph of experiment 1, the reaction time has a minimum value of 1:30 this happens at 35�C. The highest value occurs at 25�C and this is 5:00. From 25�C to the minimum value of 35�C the reaction time decreases at a decreasing rate. From the minimum value 35�C to the last value of 55�C the reaction time increases at a decreasing rate. ...read more.

Conclusion

Improvements The procedure used was simple and straightforward, however only one difficulty was encountered as mentioned before. This was the concentration of the amylase. A small improvement could have been made by testing the concentration of the amylase at the start before starting our experiment. Alternatively, our own concentrations of amylase could have been made up, as to ensure that the amylase was always the same concentration. We could of also have taken results at different temperatures to increase our range of results this would of helped dramatically to able us to a see a trend more clearly. More repeats could be taken but I don't think this would add much to the accuracy of the conclusions. More accurate equipment could have been used but again but I don't think this would add much to the accuracy of the conclusions. Extending the investigation Suggestions have already been made to extend the investigation to improve our knowledge of enzyme activity. After having previously found out about how temperature affects one particular enzyme, we could test other enzymes but according to my background knowledge most enzymes function at body temperature anyway. We could also test to see what the effects of PH have on enzyme activity and test to find out the optimum PH. Then we would have a full set of results to show the overall best conditions for enzyme activity. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Rates of Reaction - The Iodine Clock

    Result 1 163 99 60 49 38 31 Result 2 160 94 54 48 32 20 Average Time 161.5 96.5 57 48.5 35 25.5 1/TAVG 0.006192 0.010363 0.017544 0.020619 0.028571 0.039216 1/TAVG x103 6.192 10.363 17.544 20.619 28.571 39.216 Concentration KI (moldm-3)

  2. The Iodine Clock Investigation

    account the results from the initial trial experiments, the following points can now be deduced: 1. The rate at which this reaction takes place, is dependant on the concentrations of: -H2O2 -KI 2. Varying the concentration of H2SO4 had no effect on the rate of reaction, as can be seen from the results.

  1. The effect of enzyme concentration on the rate of amylase and starch reaction.

    If the fit is not exact the reaction will not occur. The diagram below shows the lock and key theory: Overall, substrate concentration will only increase the reaction rate until all of it is bound to enzyme molecules. Enzymes must bind with a substrate and form an enzyme substrate complex before products can be produced.

  2. Factors Affecting Enzyme Activity

    Seeing as no more gas was given off this suggests that the reaction is very slow and therefore apple is not a suitable source of catalase for my experiments. Next I will look at the liver suspension reaction. There was a short burst of 16 cm3 of gas into the

  1. Enzymes - show how substrate concentration affects the rate of reaction for an enzyme ...

    As result of more H2O2 broken down, there will be more product, water and oxygen produced. Variables: Variables are factors which would affect the experiment. Some variables can be controlled such as temperature whereas some variables cannot be controlled such as rate of reaction.

  2. Investigating the effect of enzyme concentration on the hydrolysis of starch with amylase.

    Amylose molecules are typically composed of 200-2000 anhydroglucose units. Aqueous solutions of amylase are very unstable because of its molecular attraction and association of neighbouring amylose molecules. This results in increased viscosity, and in particular environments, precipitation of amylose particles.

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    In order for this investigation to be carried out, I must change the concentration of bile salt observing the affect it has on the reaction. Refer to table one concentration of bile salt.

  2. Activity of Diastase On Starch

    These results shows that the rate of hydrolysis of starch is greater in acidic pH near 4 and 5 and as the pH increases from acidic to basic pH the rate of hydrolysis of starch by diastase decreases. Effect of change in temperature on the rate of hydrolysis of starch

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work