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Investigate the Effect of the Enzyme Catalyse On Hydrogen Peroxide

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Introduction

To Investigate the Effect of the Enzyme Catalyse On Hydrogen Peroxide Introduction The aim of this experiment is to determine the effects of varying enzyme (catalyse) on Hydrogen Peroxide. Hydrogen Peroxide + Catalyse --> Water + Oxygen 2H2O2 --> H2O + O2 + Heat Apparatus & Diagram Using the Equipment Safely It is important that we use the apparatus carefully, as safety will be an issue throughout the whole experiment. We will wear goggles and an apron or lab coat to protect our eyes and clothes. As we are using enzymes and Hydrogen Peroxide we need to be extra careful, ensuring they don't come into contact with our eyes, skin or clothes. Catalyse is an enzyme found in all living cells. It makes Hydrogen Peroxide decompose into water and Oxygen. We will be measuring the amount of Oxygen released from the Hydrogen Peroxide. In order to do this we will use a measuring cylinder. This piece of apparatus measures the amount of Oxygen given off, measuring in cm3. This will help us measure the amount of Oxygen more accurately. ...read more.

Middle

Investigation Experiment I am going to investigate how the enzyme, Catalyse reacts at different surface area sizes. The procedure for the experiment is as follows; * Set up the apparatus * Place the water in the water bath * Fix the inverted measuring cylinder so that the delivery tube connects to the boiling tube * Using a measuring cylinder, measure 10ml of Hydrogen Peroxide * Measure 5sm of potato and add to the Hydrogen Peroxide solution in a boiling tube * Immediately place the bung on the boiling tube, as the reaction would have already started * Set the timer for 3 minutes. Repeat the experiment three times (for accuracy) using the different pieces of potato * It is important only the size of the potato is changed, since that is what is being investigated Results Time (Secs) Attempts One Piece Two Pieces Three Pieces Four Pieces Five Pieces 30 1 1 1 2 2 3 2 1 1 2 2 1 3 1 1 2 1 4 Average 1 1 2 1.67 2.67 60 1 3 2 5 7 5 2 3 2 5 5 9 3 5 3 5 5 7 Average 3.67 2.3 ...read more.

Conclusion

Almost everything went according to plan. We followed our safety instructions and did the experiment fairly and accurately as possible. Therefore the majority of my results came out to be reliable and accurate. Due to using the equipment safely and accurately this experiment has been successful. However, there were a few results in the experiment that do no agree to the scientific information. When measuring the amount of Hydrogen Peroxide, we may have not measured the exact amount. We could have accidentally measured 10.1ml; instead of 10ml. another problem was the temperature of the room. As we carried out the experiment on different days, some times the temperature in the room varied, from 23C to 30C. This may have affected the particles in the enzymes, causing the reaction to occur slightly faster than it should or slightly slower. Luckily there were few spillages of Hydrogen peroxide, as we used the equipment safely and accurately. There was air/water in the delivery tube prior to the reaction which may have delayed the oxygen getting through. Also the condition and variety of potato could have affected the results because some the potato could have been four weeks old and the others only just freshly cut. ?? ?? ?? ?? Saeed Ahmed Biology ...read more.

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