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Investigate the effects of a named variable or the action of the enzyme trypsin.

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Biology Coursework- Enzymes Background information: Aim: The aim is to investigate the effects of a named variable or the action of the enzyme trypsin. Prediction: The factors which will affect the enzyme trypsin are the amount of water you put in, the temperature and the amount of trypsin you put in. I will be investigating the effects of trypsin at a variable temperature. I predict that the enzymes will work best at body temperature because our body stays at our temperature 37�C. The enzymes in our body have been adapted to work best at this temperature. I predict that the enzymes will denature at about 90�C. This is because the enzymes get unstable and then stop working. I predict that the enzymes will not work best at a low temperature as enzymes need at least 40�C in temperature to work. I predict that the temperature goes past body temperature the rate of the trypsin will decrease. This will happen because the enzyme will denature after 37�C. ...read more.


To make it a fair test we have to measure the water we put in which was 100cm�. We got the temperatures of water by boiling water in a kettle. For low temperatures we let the water cool down or we poured some water out and put in some cold tap water leaving it 100cm�. We then got some trypsin and put 5cm� in a test tube. We also put 5cm� of water in the test tube to give it the trace elements it needs as the concentration isn't as same as the concentration of enzymes in our body. We then put a piece of photographic film in the test tube with trypsin then put it in the beaker with the set temperature of water. As we put the test tube in the beaker we will observe the photographic film to see when the film turns transparent. We did this for 10, 20, 30, 40, 50, 60, 70, 80 and 90�C. ...read more.


Our prediction was wrong in the sense of it working at body temperature. This was because the concentration of the enzyme solution was different to that of the concentration in our body. Conclusion: In conclusion we found out that the enzymes worked best at high temperatures and not at body temperature due to the difference in concentration level. We got one odd result on graph 3 as the last point of result shows that it goes takes longer for the film to turn transparent at 90�C. This anomaly might of occurred because a window might have been open making the water turn cooler quicker or we might of forgotten to take the temperature of the water. Overall the average time tells us that the higher the temperature the faster the enzyme will work. Evaluation: We could use a number of improvements to our method as we could use a concentration of an enzyme solution close to the enzymes in our body. I don't think our results show a firm conclusion as it did not obey the rules of biology in the sense of the enzymes did not work best at body temperature and it did not denature at 90�C. ...read more.

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