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Investigate the lowest concentration of copper (II) sulphate that will bring about the full denaturation of egg albumen.

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Introduction

Investigation into the effect of concentration of copper (II) sulphate on the denaturation of egg albumen The aim of this experiment is to investigate the lowest concentration of copper (II) sulphate that will bring about the full denaturation of egg albumen. I will achieve this by diluting 0.1 mol dm3 copper (II) sulphate ten times to acquire 10 consecutively more dilute solutions. These will then be added to the same volume of egg albumen. The experiments will be performed at room temperature and the extent of denaturation will be decided on the 'opaqueness' of the albumen. Prediction I predict that the lowest concentration of copper (II) sulphate will be around 0.01 - 0.02M. This is because of the following reason. Copper ions in copper (II) sulphate are highly electropositive. Within proteins exist ionic bonds, which form between amine and carboxylic terminals resulting in polarised R groups. ...read more.

Middle

The variables used in this plan also provide a sufficient range. Equipment Black card, 3cm3 syringe, graduated pipette, ten test tubes, test tube rack, beaker, stopwatch and goggles Method 1. Measure out 2ml of egg albumen into a test tube using a syringe 2. Add 10ml of 0.1mol dm3 copper (II) sulphate into the same test tube 3. Allow the solution to stand for 5 minutes 4. Place black card behind test tube 5. Note the opaqueness of the albumen Note: The initial undiluted experiment should act as a guide. This experiment will bring about full denaturation and the opaqueness in THIS variable should be used against the others as a comparison, see 'Preliminary Tests' When repeating the experiments using different concentrations the following addition to the method must be followed BEFORE step one. 1. Fill a beaker with distilled water The following change to step 2 must also be observed. ...read more.

Conclusion

The different concentrations of solutions were decided for, as they are easy to prepare and provide a wide enough range of variables to make the experiment more accurate and give it enough depth. The black card is designed to give a more accurate estimation of the extent of denaturation as it provides a contrasting background. Risk assessment Copper (II) Sulphate (Toxic) - Wear goggles and wash hands if solution comes into contact Water spillage from baths could cause surfaces to be slippery - Wipe down wet surfaces Results Table My results will be obtained and recorded in a table similar to the one below. Concentration is measured in 'M' (mol dm-3) and the opaqueness of the albumen is recorded as a tick wherever full denaturation takes place. Concentration (M) Full Denaturation? (Tick) 1 2 3 0.1 0.09 0.08 0.07 0.06 0.05 0.04 0.03 0.02 0.01 0.00 Sources of information * Cambridge Advanced Sciences - Biology 1 * Preliminary tests 1 ...read more.

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