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Investigating enzyme activity

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Introduction

INVESTIGATING ENZYME ACTIVITY AIM The aim of this experiment is to see how changing the concentration of Hydrogen Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. The reaction I am looking at is the breakdown of Hydrogen Peroxide into water and oxygen by the enzyme Catalase. The equation for this reaction is: 2H2O2 --> 2H2O + O2 FACTORS In this experiment I could change the following factors: Concentration of the Hydrogen Peroxide or Catalase, the time, the temperature and the Ph of the solutions. SCIENTIFIC KNOWLEDGE (See appendix 1) PREDICTION My prediction is that the higher the concentration of Hydrogen Peroxide, the greater the amount of oxygen given off will be within a period of time. The lower the concentration of Hydrogen Peroxide the amount of oxygen given off will be less than if it was at a higher concentration. I think this because there are more particles in a substance to collide and react at a higher concentration. VARIABLES I am not going to change anything other than the concentration of the Hydrogen Peroxide. ...read more.

Middle

0.5% 1.0% 1.5% 2.0% 2.5% 3.0% PRELIMINARY WORK From undergoing some preliminary work I have realized that shaking the conical flask whilst the hydrogen peroxide and Catalase are in it affects the results dramatically. Therefore I have decided to keep the flask perfectly still to get full potential from the results. SAFETY Safety is a very important aspect while doing any experiment. In this experiment, in order to keep it reasonably safe I am going to wear safety glasses / goggles at all of the times. I will also be very careful whilst handling Hydrogen Peroxide as it can be a very corrosive substance. BIBLIOGRAPHY Biology - A functional approach (M.B.V ROBERTS) GCSE Biology revision guide - (CGP BOOKS) www.bbc.co.uk/schools/gcsebitesize/biology/humans\enzymes Science for GCSE Double award - (GRAHAM HILL) www.s-cool.co.uk/gcse/biology/enzymes ANALYSING TRENDS AND PATTERNS From the graph (see appendix 3), I can say that as the concentration of Hydrogen Peroxide increases, the volume of oxygen collected gets greater. Looking at the graph, Between the concentrations of 1.5% and 3.0%, I can see that when the concentration of Hydrogen Peroxide is doubled, the amount of oxygen collected also roughly doubled. ...read more.

Conclusion

I was a bit concerned when between the concentrations of 2.5% and 3.0% the volume of oxygen given off had a range of 30 cm3, whilst between the other concentrations on average the range was about 10 cm3. PROPOSED IMPROVEMENTS During the experiment I could improve the following: 1. The method of filling the burette with water rather than inverting a full burette I could fill it from the top whilst it was already inverted in the water trough. 2. Use higher concentrations ranging from 3.0% to 6.0%. This would enable me to see if the amount of oxygen produced continues to increase when the concentration of hydrogen peroxide increases. I do not believe that this would happen as all the active sites will be used up and the line on the graph will start to curve as the concentrations increase. 3. I could also check that the gas given off was definitely oxygen by putting a glowing splint into the burette. In the presence of oxygen it would re light. I could extend my investigation by including other variables such as changing the Ph or temperature instead of changing the concentrations of Hydrogen Peroxide. VAN KEMPEN ...read more.

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