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Investigating Enzymes.

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Investigating Enzymes Lab Report Research Question: Determine the Vmax and Km of an reaction with an enzyme. Does Acid affect the enzyme and the rate of reaction? Variables: The first table will suggest the variables identified for the first experiment. Independent Dependant Controlled Guiacol Rate of Reaction Amount of Enzyme Amount of Water and amount of H202 The next table will show variables for the second part of the experiment Independent Dependant Controlled HCL Rate of Reaction Enzyme Hydrogen Peroxide Guiacol Total Volume Hypothesis: For the first experiment I predicted that the amount of Guiacol has a definite effect on the rate of reaction. I predicted that the more the Guiacol in the reaction the faster will the reaction rate be. For the experiment with the HCL if predict that the enzyme will be affected by the acid, I predict that the more HCL the faster the rate of reaction. Background Information: In this experiment we are dealing with the following reaction. 4 C2H8O2 + 4 H2O2 --> 8H2O + C8H24O8 In this experiment we are dealing with the enzyme perioxidase. This enzyme is a protein which act as an catalyst for the reactions between a number of other reactions between substances. ...read more.


Design different configurations of substance with the total volume making up 2.1 ml 4. Calibrate the colorimeter by placing a blank cuvette in and taking readings. 5. Set the Colorimeter to a blue wavelength as it is the most appropriate for this experiment. 6. Make the solution with the desired configuration but leaving out one reactant thus either the Guiacol the enzyme or the hydrogen peroxide. 7. Add the reactant left out when you are ready to insert the cuvette into the colorimeter right after you have inserted the reactant. 8. Record how much time went by between inserting the reactant and the colorimeter giving it's first reading. 9. Record data for approximately 30 seconds with intervals of 5 sec each. Meaning you will have 6 results. For the experiment with HCL follow the same steps and just add HCL adjust the volume of water added to compensate for the adding of HCL. Results: The following table will show our different configurations that we used of the substances. Experiment No. Hydrogen peroxide Guaiacol Enzyme Water Total Volume (mL) 1 0.1 0.1 0.1 1.8 2.1 2 0.1 0.2 0.1 1.7 2.1 3 0.1 0.3 0.1 1.6 2.1 4 0.1 0.4 0.1 1.5 2.1 5 0.1 0.6 0.1 1.3 2.1 6 0.1 0.8 0.1 1.1 2.1 The next tables will illustrate our results. ...read more.


When I look at the results we obtained from the experiment with HCL then I notice that rate of change is not smooth at all. This let me to conclude that the acid might have influenced the enzyme. The only two possible influences there can be is that there could be competitive and non-competitive inhibitors present. This will affect the performance of the enzyme dearly and will cause it to catalyze the reaction less. Evaluation: I feel that the process which we undergone in this lab is excellent. We were really prepared for what was coming because we practiced how to use the colorimeter and how to use the suction tubes. This made the experiment a huge success. I feel that my contribution to the experiment has also led to the success of our group. I made in my goal to keep the team motivated and to double-check all the configurations of reactant so that we could eliminate as much error as possible. For further study I would definitely recommend that more acids should be used and that bases could also be used to see what there affect might be. I would also recommend to experiment with the differences in a reaction with a competitive inhibitor and in the other a non competitive inhibitor Prieur du Plessis 11A Chemistry_______________________________________________________________ ...read more.

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