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Investigating factors that affect the rate of catalyse activity on potato tissue.

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Introduction

INVESTIGATING THE FACTORS THAT AFFECT THE RATE OF CATALYSE ACTIVITY IN POTATO TISSUE ROOP THAKER - FORM 11SM - GROUP 11O Contents Page Page 1 - Title Page Page 2 - Contents Page Page 3 - Background Knowledge Page 4 - Background Knowledge Page 5 - Background Knowledge Page 6 - Background Knowledge Page 7 - Background Knowledge Page 8 - Background Knowledge Page 9 - Prediction Page 10 - Safety Precautions Page 10 - Apparatus Page 11 - Method Page 12 - Diagrams Page 13 - Results Page 14 - Graph of Water-Bath at 22�C (computer) Page 15 - Graph of Water-Bath at 22�C (hand-drawn) Page 16 - Graph of Water-Bath at 31�C (computer) Page 17 - Graph of Water-Bath at 31�C (hand-drawn) Page 18 - Graph of Water-Bath at 41�C (computer) Page 19 - Graph of Water-Bath at 41�C (hand-drawn) Page 20 - Graph of Differences between Graphs (computer) Page 21 - Graph of Differences between Graphs (hand-drawn) Page 22 - Evaluation Background Knowledge Catalase is an enzyme, it is the 'fastest' known, and i.e. it has the greatest turnover rate. Catalase exists in all aerobic cells because during respiration H2O2 which is very toxic. Therefore catalase is required so that the H2O2 can quickly be converted to water and oxygen We are going to experiment the effect of the concentration of the H2O2 on the rate of reaction in which oxygen and water is produced. What is the rate of reaction and how is it measured? ...read more.

Middle

The optimum temperature does not have to be 37�C, as the reaction s not taking place inside a warm-blooded animal, the maximum temperature does not need to be 37�C. there is an optimum temperature, although we are not sure what it is exactly. As we are using potato instead of liver, the optimum temperature would be 37�C, but not for the potato. What are molecules doing at such a low temperature? (Practical theory) Collision Theory: The higher the temperature the more the molecules can move about and the more often they can. Therefore, more successful collisions take place and results in a higher rate of reaction. Reactions in which large molecules are built up from smaller molecules are called anabolic reactions (as shown below) - a 'building up' reaction (anabolic) Enzymes fit exactly with its substrate (the molecule it acts on). They have to fit together for them to work. Too much energy will deform the enzyme. Things like high temperatures and extreme pH's will cause this. At one point, the enzyme will cease to work. The various factors will effect the reaction in different ways and will give different results. The temperature results, when plotted, would give a graph like... Before the optimum temperature is reached, the rate increases as the temperature rises, but at the optimum temperature, the enzyme de-forms. This means that the rate of reaction stops. This is why the line after the optimum is a straight line down. The pH results, when plotted, would give a graph like... ...read more.

Conclusion

3. Prepare 5 water-baths at the following temperatures: o Room temperature - 17�C--> 22�C o Water bath set at 30�C o Water bath set at 40�C o Water bath set at 50�C o Water bath set at 60�C 4. Set up the apparatus with the measuring cylinder upside down, filled with water in a container full of water. 5. Connect the delivery tube underneath the measuring cylinder in the water. 6. Place tube 1 into the room temperature water-bath, tube 2 into the 30�C water-bath, tube 3 into the 40�C water-bath, and so on. 7. Leave them in their own baths for 5 minutes till they reach the temperature of the water-bath. 8. Read the temperature of the water-bath, as it is not always exact to the nearest 10�C. 9. After 5 minutes, add the potato sample to the tube with the hydrogen peroxide, and cover it with the bung connected to the delivery tube. Start measuring the rate at which the reaction takes place. 10. Note down the results. 11. Repeat for each of the other boiling tubes in the different water baths. Diagrams Results I was not able to obtain many results but I did get these following ones: Volume of O2 produced (ml�) Temperature (�C) Time Water-Bath Water-Bath Water-Bath (mins) 22�C 31�C 41�C 1 8 ml 8 ml 17 ml 2 9 ml 10 ml 19 ml 3 11 ml 12 ml 21 ml 4 12 ml 13 ml 24 ml 5 13 ml 14 ml 27 ml (I have drawn graphs for these results to show the increase in rate and to show what happened as the reaction took place.) 1 ...read more.

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