• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating how temperature effects the rate of enzyme reaction

Extracts from this document...


Investigating how temperature effects the rate of enzyme reaction I have been asked to investigate something which effects the rate of an enzyme reaction. Temperature, concentration of enzyme, ph, concentration of substrate, light, quantity of enzyme and quality of substrate all effect the rate of enzymes. I chose to write about how temperature effects enzymes because we've done about it at school. Apparatus list Timer (stopwatch) Measuring cylinder Water bath Thermometer Rubber tube Sidearm flask Gas syringe Bung Peroxide-enzyme Balance Step by step method Firstly, you have to check the weight of the Peroxide enzyme on the balance, we need 5g Peroxide. You then put 5g Peroxide enzyme in a sidearm flask. The sidearm flask should be attached to the gas syringe. In the measuring jug you put in 10ml Hydrogen Peroxide, we use 10ml because it would be inappropriate to use more because the particles would be too much. Then put 10ml Hydrogen Peroxide in a measuring cylinder. Quickly put the sidearm flask into the water bath (the water should be a certain temperature depending on the temperature you want to do) ...read more.


The 42� temperature ranged from 6ml-20ml, the average score was 12ml. The 51� temperature ranged from 8ml-72ml, the average score was 22.4ml. Explanation The peak temperature for the reaction is 30�. It is the peak temperature because it has the most energy at this point. It does this because the higher the temperature the more energy the particles have, so the particles have more energy, so they collide more and they make more gas. This is because enzymes have a part called the active site, molecules with the same shape as the active site, molecules with the same shape as the active site collides with the enzymes active site joins them up then the substrate separates them to make h2o and o2. This is the lock and key method. The lock and key diagram: After 30� the shape at the active site is changed because of the heat. This causes the substrate and enzymes to connect together. This causes less gas to be made because they cannot react. This is when an active site changed, it is called denaturing. ...read more.


52�c and 30�c are not reliable because there is a big gap between the error bars highest and lowest reading compared to the average. I think its unreliable because the gas syringe we used was different every lesson, sometimes they were very loose and gave high readings and some were very stiff and gave low readings. If we used temperatures below 0�c and above 50�c-100�c we could have worked out whether or not the pattern is the same as the pattern on my graph. This would have been an improvement because my readings would be more accurate. At the moment we have a pattern that high and low temperatures have low reaction and in the middle temperatures have a high reaction but we need to be certain that temperatures below 0�c the reaction goes down and above 30�c it denatures. We could heat enzymes to 100�c, then cool it down to 24�c then test it to see if it still works and doesn't make gas (denatured). If it does not work it will not be denatured and will still make gas. The number of degrees was more than adequate at 10�c. The range of the temperatures was fine for this investigation I choose to carry out. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Living Things in their Environment section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Living Things in their Environment essays

  1. Marked by a teacher

    Research question - Is using dogs for work ethical?

    5 star(s)

    What was interesting was that half of the people who have dogs (1 out of the 2 people) said it was appropriate whereas the 3 people who I questioned that didn't have dogs all thought it was appropriate. This is interesting because as I wrote in my conclusion, I should

  2. Marked by a teacher

    In this experiment, mung bean seedlings and Brine shrimp eggs were used to study ...

    4 star(s)

    Replicates Replicates are important in order to increase the accuracy of the experiment. The experiment on the germination of mung bean seedlings should be repeated at least three times for each temperature in order that the accuracy of the experiment can be increased.

  1. Marked by a teacher

    Biology- enzyme coursework

    4 star(s)

    Amylase enzymes at 0�C will not have enough energy for the starch to enter the enzyme in order to make a reaction and will not work. This is why the starting temperature is 20�C. Biological Knowledge An enzyme is a protein molecule which speeds up chemical reactions.

  2. The effect of osmosis on potatoes

    Using a set of scales, I (as precisely as possible) weighed and recorded the mass of each of the three pieces of potato, trying to ensure they were all of a similar weight. Then I measured, using a measuring cylinder, 30cl of each liquid (1m, 0.6m and 0m in concentrates of sucrose solution)

  1. An Investigation into the water quality of the River Banwell in

    They test the biochemical oxygen demand, ammonia and dissolved oxygen along with nutrients nitrate and phosphate. They also look at the biological aspects that affect the life in the river. The lowest grade in any of the three tests becomes its overall grade.

  2. Human activities can impose far-reaching effects on the environment

    Thus, the overuse of persistent pesticides may lead to accumulation of these compounds in food chains. The top carnivores consume many organisms from lower down the food chain, and so ingest high levels of pesticide. Other organic molecules from the prey are respired, so the relative concentration of the pesticide increases.

  1. The comparison of bacterial content in a range of milks.

    This has improved my aseptic techniques for microbiology. (The preceding techniques require the use of special culture dishes named petri dishes or plates after their inventor Julius Richard Petri.) The most efficient method of culturing the bacteria would be the spread plate technique.

  2. To see how Blowfly larvae (Calliphora) react to light.

    a region of the paper circle where the intensity of the light is not as strong, generally moving into the negative region, with a few in the neutral zones. The reason for this is that the larvae needs to avoid the possibility of becoming prey to a predator, just before it is due to go into the pupal state.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work