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Investigating how temperature effects the rate of enzyme reaction

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Introduction

Investigating how temperature effects the rate of enzyme reaction I have been asked to investigate something which effects the rate of an enzyme reaction. Temperature, concentration of enzyme, ph, concentration of substrate, light, quantity of enzyme and quality of substrate all effect the rate of enzymes. I chose to write about how temperature effects enzymes because we've done about it at school. Apparatus list Timer (stopwatch) Measuring cylinder Water bath Thermometer Rubber tube Sidearm flask Gas syringe Bung Peroxide-enzyme Balance Step by step method Firstly, you have to check the weight of the Peroxide enzyme on the balance, we need 5g Peroxide. You then put 5g Peroxide enzyme in a sidearm flask. The sidearm flask should be attached to the gas syringe. In the measuring jug you put in 10ml Hydrogen Peroxide, we use 10ml because it would be inappropriate to use more because the particles would be too much. Then put 10ml Hydrogen Peroxide in a measuring cylinder. Quickly put the sidearm flask into the water bath (the water should be a certain temperature depending on the temperature you want to do) ...read more.

Middle

The 42� temperature ranged from 6ml-20ml, the average score was 12ml. The 51� temperature ranged from 8ml-72ml, the average score was 22.4ml. Explanation The peak temperature for the reaction is 30�. It is the peak temperature because it has the most energy at this point. It does this because the higher the temperature the more energy the particles have, so the particles have more energy, so they collide more and they make more gas. This is because enzymes have a part called the active site, molecules with the same shape as the active site, molecules with the same shape as the active site collides with the enzymes active site joins them up then the substrate separates them to make h2o and o2. This is the lock and key method. The lock and key diagram: After 30� the shape at the active site is changed because of the heat. This causes the substrate and enzymes to connect together. This causes less gas to be made because they cannot react. This is when an active site changed, it is called denaturing. ...read more.

Conclusion

52�c and 30�c are not reliable because there is a big gap between the error bars highest and lowest reading compared to the average. I think its unreliable because the gas syringe we used was different every lesson, sometimes they were very loose and gave high readings and some were very stiff and gave low readings. If we used temperatures below 0�c and above 50�c-100�c we could have worked out whether or not the pattern is the same as the pattern on my graph. This would have been an improvement because my readings would be more accurate. At the moment we have a pattern that high and low temperatures have low reaction and in the middle temperatures have a high reaction but we need to be certain that temperatures below 0�c the reaction goes down and above 30�c it denatures. We could heat enzymes to 100�c, then cool it down to 24�c then test it to see if it still works and doesn't make gas (denatured). If it does not work it will not be denatured and will still make gas. The number of degrees was more than adequate at 10�c. The range of the temperatures was fine for this investigation I choose to carry out. ...read more.

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