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Investigating how the temperature can affect the Rate of Reaction.

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Introduction

Biology - Investigating how the temperature can affect the Rate of Reaction Aim To investigate the effect of enzyme concentration on the rate of reaction the enzyme controls, using amylase and starch to illustrate this. Hypothesis I predict that the more concentration of amylase there is added to the solution, means the more number of particles there would be. This would mean the number of collisions would increase so the time would decrease as the concentration of amylase increases Equipment used - Boiling Tubes X 5 - to conduct experiments in - Test Tube rack - to hold up the test tubes - Large Syringe - to measure 5cm� of starch - Small Syringe - to measure different amounts of Amylase - Pipette - to measure precisely the volume used in each experiment is the same - Iodine Solution - used as a test for starch - Benedict Solution - used for reducing sugars (glucose, maltose etc) - Amylase - - Starch - - Spotting Tile - Stop Clock Method Firstly, 50cm� of sodium thiosulphate, measured using a measuring cylinder, is heated or cooled either using a Bunsen Burner or Ice, to reach a certain temperature. ...read more.

Middle

Also if the amount of sodium thiosulphate was more, it will affect the experiment, because the more sodium thiosulphate in the experiment, the more particles, meaning it will affect the Rate of Reaction. Safety Test When dealing with acids in a lab, you should always wear goggles in case there is a spillage, which could be very harmful and cause the Acid to burn your skin and eyes, Another basic rule when in the lab working with Acids is, never run around. This floor could be very slippery and if your holding acids you could harm yourself and others working nearby. Always tie your hair when doing practical experiments, especially when working near a Bunsen Burner. You could get distracted for a moment and the situation could be fatal. Observation Here are the Results I have collected from my experiment in the form of a table: Table Table showing my First set of Results at Different Temperatures Temperature (�C) Time (Seconds) 10� 51.55 15� 45.02 20� 25.28 25� 24.75 30� 23.43 40� 06.53 50� 05.63 60� 04.18 From Table 1, it is clear to see that there is a steady decrease in the amount of time taken for the solution to turn cloudy. ...read more.

Conclusion

Another problem I encountered was with the stop clock, which made the test very unfair as each time I started the experiment I kept pressing the wrong button, and by the time I got it right, the experiment had already turned cloudy. To make the experiment more accurate next time, I should let one partner do the timing, and let the other pour the hydrochloric acid into the beaker. The test could have also been more accurate if I repeated the experiment a couple more times, thus getting a more accurate result or even by making the temperature go up by 2�, where I would have been able to see where exactly the Rate of Reaction begins to speed up and at what Temperature. Also, a burette could have been used to measure the volume more accurately, to give a more precise volume. This test may have also been unfair due to the lack of accurate equipment. Overall, I am pleased with my results and findings, and have a better knowledge of how a Reaction such as Sodium thiosulphate and Hydrochloric Acid can be affected by not just the surface area, a catalyst and the concentration, but also the temperature. Nina Hurhangee 11 Miranda ...read more.

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