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Investigating industrial processes - rates of reactions of enzymes

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20th / September/2002 INVESTIGATING INDUSTRIAL PROCESSES: RATES OF REACTIONS OF ENZYMES Plan: I am going to use the following apparatus: 1.Test tubes 2. Protease enzyme 3. Syringes 4. Squares of photographic film with gelatine in it. 5. Splints 6. Stop watches 7. pH solutions I am going to do the following: 1. Put different pH solutions provided into different test-tube. (In this case pH 5- 9 solutions) 2. Put equal amounts in volume of protease solution into the different test tubes bearing different pH solutions in them approximately 5. 3. Using splints take the photographic films, which are smeared with gelatine and dip them into different test tubes containing the different solutions. 4. Immediately start the stopwatch and note the time at which each of the photographic films turns clear from black. 5. Draw a table and place in the results; repeat the experiment to get better results. ...read more.


s 208 s 8 368 s 400 s 240 s 336 s 9 1372 s 1535 s 1365 s 1424 s pH Time 1/s Time 2/ s Average/s 4 1560 1560 1560 6 355 348 351.5 7 255 235 245 8 368 400 384 9 1372 1535 1453.5 * As you can see above the third timing has not been used to calculate the average for the graph. * This is because there is more enzyme concentration than in any previous 2 experiments. * To get the average you: total of times = average time Number of times * To get the time in seconds you: Time in seconds = time in minutes X 60 Safety Put on lab coats to protect clothes and don't put on open shoes Put on Gloves to avoid any corrosive action hazards Put on safety goggles to avoid hazards related to the eyes Anticipation of any difficulties 1. ...read more.


3. I had planned to use PH 5- 9 but instead available was pH 4,6-9. 4. The stopwatches were hard to get used too since I would press the wrong buttons. This interfered with some results but I repeated every thing altogether. 5. There was time wastage since I had one stopcock and I had to do the experiments a number of times. Some solutions to these problems 1. When I go the results of the third experiment I just recorded them but I didn't use them to construct my graph because they would interfere with the results. 2. I did the pH values, which took short times first, and then I concentrated on the long-term experiments. 3. I used instead the available pHs and worked with them accordingly. 4. I practiced with the stop clock a number of times to get me accurate. 5. For the future experiments I suggest multiple stop clocks to be able to start off many experiments especially the long term ones at a go. ...read more.

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