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Investigating Membrane Permeability.

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Introduction

Maryam Farmani 12 SCS Investigating Membrane Permeability Apparatus:cogf gfr segfgfw orgf gfk ingf fogf gf. � 8 test tubescoea ear seeaeaw orea eak inea foea ea. � Test tube rackcodg dgr sedgdgw ordg dgk indg fodg dg! � Measuring cylinder � Bunsen burner � Knife � Beaker � 1 beetroot � Dilute hydrochloric acidcobf bfr sebfbfw orbf bfk inbf fobf bf! � Dilute sodium hydroxide solutioncobg bgr sebgbgw orbg bgk inbg fobg bg. � Ethanol abidzaman, please do not redistribute this paper. We work very hard to create this website, and we trust our visitors to respect it for the good of other students. Please, do not circulate this paper elsewhere on the internet. Anybody found doing so will be permanently banned. � Distilled watercogb gbr segbgbw orgb gbk ingb fogb gb: � Thermometer � Tongscocb cbr secbcbw orcb cbk incb focb cb. � Tripodcofd fdr sefdfdw orfd fdk infd fofd fd: � Mat lGExVw Visit coursework cd in cd fo cd for cd more hypothesis cd Do cd not cd redistribute lGExVw � Cork borer Method: 1. I first cut three discs each about 2mm thick from the cylinder, which was cut out of the beetroot using a corer and place them in a test tube containing 10cm3 distilled water. This was my test tube number1.coea ear seeaeaw orea eak inea foea ea. 2. I then cut 21 more discs and rinse them under the running water, until the pink water collected in the beaker was clear.coeb ebr seebebw oreb ebk ineb foeb eb. 3. I placed three of the 21 washed discs, in each four test tubes.cogf gfr segfgfw orgf gfk ingf fogf gf. To test tube 2 I added 10cm3 of distilled water This cours from www.coursework.info To test tube 3 I added 10cm3 of ethanol This coursework from www.coursework.info To test tube 4 I added 10cm3 of dilute hydrochloric acid wEQIk Visit coursework gd in gd fo gd for gd more dissertation gd Do gd not gd redistribute wEQIk ...read more.

Middle

to hold the beetroot securely making sure that your hand wasn't in the path of the borer, it was also important to screw the borer away from your body. The long cylinder had to then be dice using a sharp scalpel that cased less damage to the membranes, however it may have been dangerous if a person did not apply their full concentration when using. Users must be careful concerning how the scalpel was held and used.coda dar sedadaw orda dak inda foda da. Water baths were used to determine the effect of temperature on the permeability of the membrane; the water baths were set at varying temperature up to 61 degrees C the higher temperature may have been painful if splashed on the skin therefore it was essential to take care when placing the test tubes in to the bathes. This coursework from www.coursework.info There were very little risks of serious injury, any accidents which may of occurred would have been down to lack of concentration on their parts.coeb ebr seebebw oreb ebk ineb foeb eb. Results-coed edr seededw ored edk ined foed ed. Temperature oc abidzaman, please do not redistribute this project. We work very hard to create this website, and we trust our visitors to respect it for the good of other students. Please, do not circulate this project elsewhere on the internet. Anybody found doing so will be permanently banned. Test tube 1 % of light absorbedcodg dgr sedgdgw ordg dgk indg fodg dg. Test tube 2 % of light absorbed This cours from www.coursework.info Average % of light absorbed This paper from www.coursework.info 2 (Ice)cobg bgr sebgbgw orbg bgk inbg fobg bg! 1 abidzaman, please do not redistribute this project. We work very hard to create this website, and we trust our visitors to respect it for the good of other students. Please, do not circulate this project elsewhere on the internet. ...read more.

Conclusion

Also the temperature of the water baths were not set entirely accurately, which may have a similar affect as the one stated earlier. This may cause us to miss a vital reading. The method we used slightly impaired our ability to carry out more than two repeats at once, if we had been more organised and worked as a larger group more repeats may have been done. Therefore boosting the amount of individual results and also making our average more accurate. The Individuality of the beetroot may have caused our results to be different from other peoples in the class. Just like humans have different amounts of pigment in their skin so do beetroot, our specific beetroot may have contained less pigment. Therefore causing the % of light absorbed to be lower.. Effect of limitations I don't believe that these limitations will have a great affect on the over all results of our experiment, due to the fact that the results we have obtained from the original and repeat were very similar in most cases. Although there are some anomalous results, this does not seem to have affected the average as it still increases with the increase of the temperature. Which would have been expected due to the temperature increasing the permeability of the beetroot cell membrane allowing more pigment to pass through. The wider range of temperatures would have enabled us to pin point the temperature at which the cell membrane becomes denatured, which would of made the experiment more accurate, however this didn't affect our experiment a lot because we were investigating the effect of temperature on a cell membrane which can be clearly seen by our results. The reliability of our apparatus didn't affect us either because the result increase as we would of expected. The accuracy of our results could be tested against other groups or against other repeats. BGZU from ...read more.

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