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Investigating Seed Germination. Hypothesis If there is water, oxygen and a suitable temperature in the surroundings, then the seed will germinate.

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SSIS MYP Programme Science Investigation Report Investigating Seed Germination Name: Kimberly Hoong Yearn Yi Class: S4 Ruby Aim To investigate how the following conditions: presence of water, presence of oxygen, and the temperature would affect the germination of a seed. Hypothesis If there is water, oxygen and a suitable temperature in the surroundings, then the seed will germinate. A seed is an embryonic plant in a resting condition after the development of the embryo in the ovule of the plant stops. Water is then lost and the seed enters a state of dormancy, where its metabolic activities are suspended until germination takes place. In order for germination to occur, water, oxygen and a suitable temperature must be present. Water must be present, because it is what sets off the metabolic activity inside the seed. Water is taken up by a process called imbibition and is needed for the metabolism of the seed as well as for breaking the seed coat by making the seed swell. With water and all other conditions (oxygen, suitable temperature) present, germination commences with the uptake of water by imbibition of the dry seed, followed by embryo expansion. The water absorbed dissolves a chemical made inside the embryo. Hydrolytic enzymes are then activated, breaking down the stored nutrients into nourishments for the embryo. Oxygen is another important condition for germination. Before the seed can photosynthesize through its leaves, the seed has to undergo respiration in order to provide energy for germination. The seed needs oxygen so that it can respire aerobically to convert its energy stores into energy that it can use for growing. ...read more.


Set Up (3) - No Oxygen This set up will have all the conditions needed for the germination of the seed except for a supply of oxygen. Like the other set ups, it will have 3 green bean seeds on top of 4 cotton pads. It will receive 10cm3 of water each day for 7 days, and be placed in an area at room temperature. However, the seeds will be covered in a layer of oil, which will deprive the seeds of the oxygen in the air, hence drowning the green bean seeds. As oxygen is not present, the embryo will be not able to respire in order to provide energy for germination. Without energy, the seed will not be able to germinate. Set Up (4) - No Water This set up will have all the conditions needed for the germination of the seed except for a supply of water. Like the other set ups, it will have 3 green bean seeds on top of 4 cotton pads. It will be placed in an area at room temperature with oxygen. However, the seeds will only receive 2cm3 of water each day for 7 days, which will not be sufficient enough for the seeds to germinate. As the supply of water is not sufficient, imbibition will not occur and the metabolism of the seed will remain dormant. Without the metabolic activity of the seed, it will not germinate. Method 1. Lay out all apparatus, clean all of them thoroughly. 2. Set up the Petri dishes with 4 cotton pads in each Petri dish. ...read more.


I also used a 50cm3 measuring cylinder to measure 50cm3 of oil to pour onto the seeds. In addition, a vernier caliper was used to measure the length of the seedlings, which is used to measure lengths and thicknesses up to 0.01cm. It was also checked for any zero errors before it was used. The refrigerator in which Set up (2) was placed in was thermostatically controlled, so the temperature was constant throughout, and there was no need for a use of a thermometer. The method was valid as the dependent and independent variable were measured and results were obtained. The hypothesis was valid as the outcome of the experiment supported it. Limitations and Improvements If I could do the experiment again, --> One or two more seeds could be used so that I could get a better reading on the trends. In addition, I only took the final results, which is the final length of the seedlings. I could have observed the growth of all the seeds closely so that I would be able to further explain and analyze my results. --> Over the weekend, the seeds were not tended to, so during those two days there was a lack of water for the seeds. I could have brought the set up home with me so that I could tend to them with full attention instead of leaving them in school. --> During the night and day, the room temperature can vary by 4-5�C. In order to keep the temperature constant in the future, I could put it in a thermostatically controlled room or container. This would ensure that the temperature will remain constant throughout, hence not affecting the seeds' germination. ?? ?? ?? ?? 1 | Page Science Investigation - Seed Germination ...read more.

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