• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of Catalyse on Hydrogen Peroxide.

Extracts from this document...

Introduction

Investigating the Effect of Catalyse on Hydrogen Peroxide Aim To investigate the effect of Hydrogen Peroxide on a Catalase enzyme, whilst heating the reaction at different temperatures. Background Knowledge An enzyme is a protein that is synthesised by the body. They have a special optimum temperature that when reached, provides the ideal conditions for 100% efficiency. If the temperature rises above its specific temperature, then the enzyme fails to do its job as well. This is called 'Denaturisation'. Enzymes in the human body work best at 37.5�C, and if our temperature rises, then we sweat, and if it falls, we shiver. Here is a hypothetical graph to show this concept. If conditions are out of the specific range, then the enzyme may become denatured, which means that the enzyme will not perform its job properly. This is because of the 'Lock and Key' concept. The lock and key concept is a way of showing how the substrate (key) fits into the enzyme (lock). This reaction shows the activity of enzymes in the body, and how they work: Enzyme + Substrate � Enzyme-substrate Complex � Enzyme + Products Using the lock and key concept, the above reaction can be shown as a series of diagrams: Substrate Enzyme Enzyme Substrate Complex Products Enzyme Therefore, ...read more.

Middle

Analysis The results that I have collected show me that when an enzyme is heated to a specific temperature, it produces more oxygen than at another temperature. Using my first graph, I can say that in this case, the piece of liver produced about 22.5mm3 of oxygen at 35�C and at 50�C it only produced about 5mm3 of oxygen. From my first graph I can see that the enzymes optimum temperature is 22.5�C. After this point, it denatures, and before this point, the enzyme is not working as well as what it could be. My results from this graph are quite accurate and there does not appear to be any anomalous results. As you can see, I stopped recording results at 60�C. This proves to be a very good point to stop as the enzyme looks as if it is producing very little oxygen. My second graph shows the rate of the reaction and is more or less the same shape as my first graph. This graph shows that the enzymes was reacting its best at about 37�C, and that after this point, the enzyme denatured. This is what my first graph also shows. ...read more.

Conclusion

That is to say that at the optimum temperature was roughly the same and the enzyme begins its activity at the same point (roughly). My aim, 'to investigate the effect of Hydrogen Peroxide on a Catalase enzyme, whilst heating the reaction at different temperatures' has been reached. I have done this by using Hydrogen Peroxide and pieces of liver, and I have done it at a number of temperatures. I think that the suit I can also say that I was right with my prediction, as I said that I thought that if the temperature of the reaction were increased, then the amount of oxygen released from the liver would also increase. This has proved to be true. I also said that this would be because an enzyme works a lot better under a specific temperatue, which I found out to be 37�C. I then said that if the temperature was raised to far, then the enzyme will denature and so the piece of liver will not produce as much oxygen. This is also correct, as I have found out using different temperatures to measure the amount of oxygen produced. I lastly said that if the temperature were raised above an enzymes optimum temperature then the key would be reshaped and therefore would not be able to fit into the lock. Shaun May: 9083 Chatham Grammar School for Boys: 61201 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Investigating the breakdown of hydrogen peroxide by the enzyme catalyse in potatoes.

    4 star(s)

    If after writing the tables we notice that there is an anomaly we would highlight that field and then repeat the test so that the results would be more accurate. This happened to us on a couple of occasions and we were able to spot it as the range of the outlier was different to the other two results.

  2. Marked by a teacher

    To investigate the effect of temperature on enzyme activity. The enzyme used will be ...

    4 star(s)

    cubic centimetres catalyse (in the form of potato), 30�C, 40�C, 50�C, 60�C and 70�C Water Baths, ice, three 5 cubic centimetre syringes, and a thermometer. Method: The following measurements were used in a previous trial experiment to see whether they were effective.

  1. The effect of hydrogen peroxide on catalase if you change the temperature.

    Kinetic theory states that particles, which gain heat energy, move more quickly. In our case the reacting particles are the substrate (hydrogen peroxide) and the enzyme catalase. As the temperature is increased the particles of hydrogen peroxide have more energy therefore they collide with the potato more frequently and so increasing the rate at which the product is formed.

  2. Investigate the Effect of the Enzyme Catalyse On Hydrogen Peroxide

    We will be measuring the two main factors: - * Oxygen - the amount released from the Hydrogen peroxide * Time - The duration of the reaction The room temperature may be a factor that will affect our results. Therefore we will have to try our bests to inure the test is carries out under the same conditions.

  1. The effect of substrate concentration on the activity of catalyse.

    Temperature As the temperature increases, the molecules gain kinetic energy and move around faster. After an optimum temperature however the rate of reaction decreases as the active site denatures. This factor has to be controlled to ensure that all reactions occur at optimum temperature.

  2. Enzyme Investigation. How to find the best concentration of hydrogen peroxide that will react ...

    Clean your desk and put all of your equipment away and wash your hands Results for pre-test: Time (seconds) Volume of O� produced (cm�) pH 30 60 90 120 150 180 210 240 4 12 15 21 26 29 34 40 43 14 19 24 29 32 37 43

  1. An investigation to see how much oxygen was produced in the reaction between Hydrogen ...

    * Delivery Tube-to let the oxygen gas pass through. * Stop clock- to time the 2mins in which the reaction takes place. * Goggles- to protect your eyes from irritation. * Ice cream container-to hold the water. * Ruler- to measure the amount of potato. * Knife- to cut potato.

  2. Investigating the breakdown of hydrogen peroxide by catalyse.

    We then decided on a time of when we could measure the oxygen bubbles that had formed, in the firsted experiment that we tried we left a gap of three mins but then we changed it to 1 min because the experemnt took to long.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work