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Investigating the Effect of Catalyse on Hydrogen Peroxide.

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Introduction

Investigating the Effect of Catalyse on Hydrogen Peroxide Aim To investigate the effect of Hydrogen Peroxide on a Catalase enzyme, whilst heating the reaction at different temperatures. Background Knowledge An enzyme is a protein that is synthesised by the body. They have a special optimum temperature that when reached, provides the ideal conditions for 100% efficiency. If the temperature rises above its specific temperature, then the enzyme fails to do its job as well. This is called 'Denaturisation'. Enzymes in the human body work best at 37.5�C, and if our temperature rises, then we sweat, and if it falls, we shiver. Here is a hypothetical graph to show this concept. If conditions are out of the specific range, then the enzyme may become denatured, which means that the enzyme will not perform its job properly. This is because of the 'Lock and Key' concept. The lock and key concept is a way of showing how the substrate (key) fits into the enzyme (lock). This reaction shows the activity of enzymes in the body, and how they work: Enzyme + Substrate � Enzyme-substrate Complex � Enzyme + Products Using the lock and key concept, the above reaction can be shown as a series of diagrams: Substrate Enzyme Enzyme Substrate Complex Products Enzyme Therefore, ...read more.

Middle

Analysis The results that I have collected show me that when an enzyme is heated to a specific temperature, it produces more oxygen than at another temperature. Using my first graph, I can say that in this case, the piece of liver produced about 22.5mm3 of oxygen at 35�C and at 50�C it only produced about 5mm3 of oxygen. From my first graph I can see that the enzymes optimum temperature is 22.5�C. After this point, it denatures, and before this point, the enzyme is not working as well as what it could be. My results from this graph are quite accurate and there does not appear to be any anomalous results. As you can see, I stopped recording results at 60�C. This proves to be a very good point to stop as the enzyme looks as if it is producing very little oxygen. My second graph shows the rate of the reaction and is more or less the same shape as my first graph. This graph shows that the enzymes was reacting its best at about 37�C, and that after this point, the enzyme denatured. This is what my first graph also shows. ...read more.

Conclusion

That is to say that at the optimum temperature was roughly the same and the enzyme begins its activity at the same point (roughly). My aim, 'to investigate the effect of Hydrogen Peroxide on a Catalase enzyme, whilst heating the reaction at different temperatures' has been reached. I have done this by using Hydrogen Peroxide and pieces of liver, and I have done it at a number of temperatures. I think that the suit I can also say that I was right with my prediction, as I said that I thought that if the temperature of the reaction were increased, then the amount of oxygen released from the liver would also increase. This has proved to be true. I also said that this would be because an enzyme works a lot better under a specific temperatue, which I found out to be 37�C. I then said that if the temperature was raised to far, then the enzyme will denature and so the piece of liver will not produce as much oxygen. This is also correct, as I have found out using different temperatures to measure the amount of oxygen produced. I lastly said that if the temperature were raised above an enzymes optimum temperature then the key would be reshaped and therefore would not be able to fit into the lock. Shaun May: 9083 Chatham Grammar School for Boys: 61201 ...read more.

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