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Investigating the effect of copper sulphate on the rate of reaction of the break down of hydrogen peroxide by catalase.

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Introduction

Investigating the effect of copper sulphate on the rate of reaction of the break down of hydrogen peroxide by catalase. Aim: To investigate the effect of copper sulphate on the rate of reaction of the break down of hydrogen peroxide by catalase. Variables: The variables that I can investigate in my preliminary work are: > Surface area and volume of potato > Volume of Hydrogen Peroxide > Volume of copper sulphate The first variable will involve me cutting different sized pieces of potatoes. The reason for using different surface areas is that this will determine the number of active sites, for the substrates to react in. The greater the surface area of the potato the greater the number of active sites. A larger number of active sites give more opportunity for substrates to react, increasing the rate of reaction. With the second variable I will use different volumes of hydrogen peroxide most likely in gaps of 5mls. In this case the greater the volume of hydrogen peroxide the greater the number of substrates to react in the active sites. This would in turn increase the rate of reaction. After deciding the first two variables I will look at the third variable, the volume of copper sulphate. ...read more.

Middle

Safety To ensure safety when carrying out this reaction I will wear goggles at all times to protect my eyes and I will wear rubber gloves to protect my hands from accidental spills. The reason this is need is because 20 vol hydrogen peroxide can be very dangerous if it makes contact with the skin. Apparatus: Side arm conical flask Burette Bung Hydrogen Peroxide Copper Sulphate Potato Stop Clock Water bath Clamp Stand Distilled Water Method: 1. Set up the apparatus detailed above, on the diagram on the next page. 2. Place 30mls of your solution into the side arm beaker. At this point you should also add the relevant amount of copper sulphate. A table to tell you how to make each solution is detailed on the next page. 3. Place 8 equally cut pieces from a 8cm3 potato into the solution. 4. Put the bung on the top of the side arm beaker. 5. Start taking results after the big bubble from putting pressure on the bung has risen. At this point you should also start the stop clock. Write down this point on the burette as 0seconds. Take the next reading at 15 seconds and continue to take readings every 15seconds. ...read more.

Conclusion

The arm was also bent meaning arm could have been trapped in the bend. To improve my experiment I think I would use a flask with an arm that is straight and connected directly to the measuring device. I would also not use a burette but a thinner tube for more accurate reading. The apparatus and procedure was good enough to produce an accurate graph for this experiment. For the accuracy level I need my procedure was great. If I wanted to take this investigation further I could investigate other types of enzymes, i.e. maltase. I could also use different variables or a different substrate. Then I could investigate the rates of reaction of different substrates as well as different variables in this experiment. This would enable me to do a research experiment for a company who work in this business. Another thing that I would do is to use different concentrations of copper sulphate. The ones I chose were not very good for the experiment and whilst they just about support my conclusion using up to 500% copper sulphate would have been far greater. I believe this would have then halved the results I got for the 50% concentration, which would make the graph look better and enable me to analyse my results more accurately and in a more scrutinising manner. Al Gwilliam Lee 12 12/12/02 Biology Investigation Aylesbury Grammar School ...read more.

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