• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the Effect of Temperature in Catalyse Activity.

Extracts from this document...

Introduction

Aim In this experiment I will attempt to investigate the factors that effect the rate of reaction of the enzyme catalyst. Catalyse is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from the cells. Catalyse speeds up the decomposition of Hydrogen Peroxide into water and oxygen. It is able to speed up the decomposition of Hydrogen Peroxide because of the shape of the Hydrogen Peroxide molecule. This type of reaction where a molecule is broken down into smaller pieces is called an anabolic reaction. Catalyse 2H2O2 2H2O + O2 Enzymes are proteins that act as catalysts. They are made in cells. A catalyst is something that speeds up a reaction, but does not get used up in the reaction. One can usually be used many times. An example of this is shown in the following diagram: Prediction and Scientific Knowledge I predict, that if I have a higher enzyme concentration, then this will cause a higher rate of catalyse activity, because there will be more active places for a reaction to take place, which will cause more chemical reactions because of successful collisions because of the active site of the enzyme and substrate. ...read more.

Middle

produced. To insure that the temperatures were at their exact point and to make sire the we stable, we used water baths, which heat the water to the exact temperature and keep the temperature constant, as to make the test a fair one. As with all experiments, we had to be aware of safety, for a number of reasons: We were using a knife to cut up the potatoes, which, when being used wrongly could lead to injury. H2O2 is a corrosive substance, so we had to be careful that we did not come in direct physical contact with it (i.e. spill it on our skin) and finally, We were using a water of high temperature (50 and 60) so had to be careful not to burn ourselves. Table of Results Temperature (?C) Test 1 (O2 produced in cm�) Test 2 (O2 produced in cm�) Test 3 (O2 produced in cm�) Average (cm�) 20.0 (room temp) 1.20 0.80 0.90 0.96 30.0 1.40 1.80 2.00 1.73 40.0 2.40 2.60 3.10 2.70 50.0 1.80 2.00 1.50 1.76 60.0 0.07 0.09 0.10 0.086 Summary of Results You can see from the results, the reaction rate slowly increases as it goes from 20 to 40 and at 50 it has begun to denature, but by 60 the enzymes have completely denatured and the reaction is very small. ...read more.

Conclusion

The quality of the data we obtained was if not perfect, close to correct as it approximately fitted with our theoretical predictions that after 40 the enzymes would begin to denature and by 60 they would be completely ineffective in the reaction. With most experiments like this one, there are always going to be certain factors that either aren't carried out properly or are not going to work quite as they should in a classroom atmosphere, as it is far different from a scientific lab, but I feel that the experiment was suitable and we received good, accurate results. Improvements There are a few improvements that could be made to improve the fairness and accuracy of the experiment. For example: Use either a more accurate water bath, or other device that can heat to a certain temperature and keep that temperature at a constant level. And Use a more accurate instrument (rather than a knife and ruler) to cut out the potatoes to insure they are all of exactly the same length and surface area, to keep the test fair. Another thing I would have done is use a pureed potato instead of using a potato cylinder like I did. This is because it would give more active sites for a reaction to take place. Roy Vivasi ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    Pour distilled water into a beaker and place the probe inside however make sure the water is not near to the computer, for safety reasons. 3. Prepare equipment neatly on a table (table must be away from the computer table).

  2. To investigate and compare the effect of temperature on the activity of catalase in ...

    time Ice Cubes Bunsen Burner To create different temperatures for different water baths. Tripod Gauze To place beaker on Beehive shell To make sure delivery tube does not slip out of place during the experiment, which would result in huge inaccuracies.

  1. Factors Affecting Enzyme Activity

    and concentration of the enzyme solution (catalase solution). I think temperature would affect the rate of enzyme activity as all enzymes have an optimum temperature at which they work best, the reason for this is explained in the further theory section. This temperature is the temperature the enzyme was intended to work at.

  2. The effect of concentration on the activity of catalase.

    This experiment looks at the effect of concentration in the reaction between the enzyme catalase and hydrogen peroxide. Increasing the concentration increases the frequency of collisions between the reactants because there are more molecules in a given volume. This will increase the number of effective collisions and therefore the rate.

  1. To investigate the effect of varying the masses of white sugar and yeast and ...

    It was fluid and yellow. Nowadays, yeast is made commercially on a large scale. The yeast used in this study was bought at a supermarket with the yellow lumps done up in paper and compressed for convenient handling. Yeast is a plant, according to the biologists, and is capable of reproducing itself.

  2. Investigate the action of the Enzyme Catalyse

    There will be 50 active molecules that will collide and react. (This was only an example. Not actually correct) Surface Area: This is the factor that I am going to change. I will measure it as accurately as possible. The diameter will always be the same, as I will use the same cork borer for every cylinder.

  1. Rates of reactivity.

    so laboratory coats and goggles were always worn throughout the experiment. * When conducting hot liquids around, there should be no running around, so that no accidents would occur involving hot liquids. Special care was taken when transporting hot reactive metals, and when using corrosive metals in experiments.

  2. Catalase activity in the liver

    The temperature of each water bath will vary but separately the water bath will have the same temperature so they are controlled I will do this by setting each water bath at my wanted temperatures I will also be checking the water baths to see if they are at the right temperature by putting a thermometer in each water bath.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work