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Investigating the effect of temperature on the reaction between Catalase and Hydrogen Peroxide

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Introduction

Investigating the effect of temperature on the reaction between Catalase and Hydrogen Peroxide Aim; To investigate how changing the temperature affects the rate of reaction between hydrogen peroxide and the enzyme Catalase. Also to investigate at what temperature the enzyme Catalase denatures and works best. Theory/Background Info; Four things affect the rate of reaction; concentration, surface area, temperature and use of catalyst. In this experiment, I will be changing the temperature, and watching the effect it has on the reaction between the hydrogen peroxide and the Catalase. Increasing the temperature, would cause the particles in the hydrogen peroxide to move faster, causing more collisions with the Catalase, and reacting faster to produce the oxygen. At a lower temperature, the collisions are poorer because the particles are moving a lot more slowly. Also when a collision occurs, there is less chance of a reaction taking place because the movement energy in the particles is less. At a higher temperature, the number of collisions is superior because when a collision occurs, there is more chance of a reaction taking place because the movement energy in the particles is greater. Another factor, which could also be said to play a part in the experiment, is surface area. ...read more.

Middle

As enzymes denature or slow down when reacting at higher temperatures (as there shape is either destroyed or broken), I also predict that at 45�c, the reaction will either slow down or the enzyme will completely denature. Method; Firstly I collected my apparatus; a clamp stand, two measuring cylinders - one for the experiment and one for measuring the hydrogen peroxide, a water trough, a delivery tube and bung, boiling tube, thermometer and a stop clock. I set it up as below - Having set up the apparatus as shown, before placing the measuring cylinder as in the diagram - I filled the water trough with water, and then completely filled the measuring cylinder, also with water, placing it securely upside down in the water trough with the clamp stand - being sure to keep the measuring cylinder full of water. I then collected my reactants - 20cm� of hydrogen peroxide, and 1 gram of finely sliced potato - being as accurate as I could when I measured the potato (managing to keep it within 0.02 grams above or below exactly 1 gram). Having collected the potato slices and the 20cm� of hydrogen peroxide, I went to collect the water for the beaker - which would act as a water bath for the hydrogen peroxide in the boiling tube. ...read more.

Conclusion

As this is not a major change in the amount of oxygen being produced, I am not that troubled with it - as the reason for this decrease could simply be that the temperature for each of the three tests of the water bath or the hydrogen peroxide was not exact to the desired temperature - causing an anomaly in the experiment. At 45�c, the Catalase seems to have produced on average, the most oxygen within 2 minutes - it seems that it is here, that the Catalase works the best. Not having done any further temperatures may have effected seeing exactly when it is that the Catalase denatures. It would only be safe to say that most probably; Catalase has another temperature at which it works the best, other than 45�c, and it would denature at a higher temperature. Evaluation; Having done this experiment, I would have preferred to have carried it out over a longer period of time - if I could have, I would have been able to do more experiments which could have shown a wider variety of temperatures and I would be able to see the optimum temperature that the Catalase would work at, and also see at what temperature the Catalase would denature at. ?? ?? ?? ?? Kiran Toora 10me - Biology Coursework ...read more.

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