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Investigating the factors that affect the reaction between liver and hydrogen peroxide.

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Investigating the factors that affect the reaction between liver and hydrogen peroxide. Science Theory Liver breaks down hydrogen peroxide using an enzyme called catalase, a toxic product, to form water and oxygen, non-toxic product. The speed of the reaction with the enzyme is effect by several factors such as temperature, pH and concentration. The temperature that an enzyme works best at is just above body temperature because if it were directly body temperature the reaction would not be controlled. At lower temperature the enzyme slows down and thus doesn't work efficiently, at a too high temperature the enzyme is denatured and fails to react. Each enzyme has a different optimum pH, some prefer acidic and some alkali however the optimum pH for this investigation is around 7. Although it may be slightly acidic as the liver contains bile acids. ...read more.


Enzymes have an optimum environment for working this includes pH and temperature. Fair Test The things that I shall keep the same through my investigation are the amount of hydrogen peroxide and liver used, also the time that the reaction is measured for and the pH of the reaction. Apparatus 1. Liver 2. Hydrogen peroxide 3. Test tube 4. Beaker 5. Delivery tube 6. Measuring cylinder 7. Thermometer 8. Stopwatch 9. Reservoir of water Diagram Preliminary Work The preliminary work I did was to find the best amounts of hydrogen peroxide and liver. The reaction was too vigorous with 5cm� and not strong enough with 2.5cm� of hydrogen peroxide. Also using a small piece of liver instead of a paste was easier, the paste was made by grinding the liver with sand and water. Plan First I shall set up my experiment and then measure the reaction using a stopwatch and a measuring cylinder for ...read more.


After the optimum temperature the reactions then slows down again very quickly thus showing that enzymes only work efficiently at there optimum conditions. Evaluation To improve the investigation more accurate apparatus should be used. This would ensure that all the constants were kept precisely constant; this would mean that other factors wouldn't effect the reaction. More accurate apparatus would also measure the reaction a lot more precisely and wouldn't have to allow for the human reaction time such as with pouring the hydrogen peroxide, starting and stopping the stop watch and reaction. Human errors can't greatly affect the accuracy of results; reaction times vary from day to day and so are not very accurate, a computer taking the results in would be better as they are more accurate The same liver should be used and the reactions done on the same day so the liver is at the same condition for the investigation as over time the enzymes may have become less effective. Mary Death ...read more.

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