• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigating the factors that affect the reaction between liver and hydrogen peroxide.

Extracts from this document...


Investigating the factors that affect the reaction between liver and hydrogen peroxide. Science Theory Liver breaks down hydrogen peroxide using an enzyme called catalase, a toxic product, to form water and oxygen, non-toxic product. The speed of the reaction with the enzyme is effect by several factors such as temperature, pH and concentration. The temperature that an enzyme works best at is just above body temperature because if it were directly body temperature the reaction would not be controlled. At lower temperature the enzyme slows down and thus doesn't work efficiently, at a too high temperature the enzyme is denatured and fails to react. Each enzyme has a different optimum pH, some prefer acidic and some alkali however the optimum pH for this investigation is around 7. Although it may be slightly acidic as the liver contains bile acids. ...read more.


Enzymes have an optimum environment for working this includes pH and temperature. Fair Test The things that I shall keep the same through my investigation are the amount of hydrogen peroxide and liver used, also the time that the reaction is measured for and the pH of the reaction. Apparatus 1. Liver 2. Hydrogen peroxide 3. Test tube 4. Beaker 5. Delivery tube 6. Measuring cylinder 7. Thermometer 8. Stopwatch 9. Reservoir of water Diagram Preliminary Work The preliminary work I did was to find the best amounts of hydrogen peroxide and liver. The reaction was too vigorous with 5cm� and not strong enough with 2.5cm� of hydrogen peroxide. Also using a small piece of liver instead of a paste was easier, the paste was made by grinding the liver with sand and water. Plan First I shall set up my experiment and then measure the reaction using a stopwatch and a measuring cylinder for ...read more.


After the optimum temperature the reactions then slows down again very quickly thus showing that enzymes only work efficiently at there optimum conditions. Evaluation To improve the investigation more accurate apparatus should be used. This would ensure that all the constants were kept precisely constant; this would mean that other factors wouldn't effect the reaction. More accurate apparatus would also measure the reaction a lot more precisely and wouldn't have to allow for the human reaction time such as with pouring the hydrogen peroxide, starting and stopping the stop watch and reaction. Human errors can't greatly affect the accuracy of results; reaction times vary from day to day and so are not very accurate, a computer taking the results in would be better as they are more accurate The same liver should be used and the reactions done on the same day so the liver is at the same condition for the investigation as over time the enzymes may have become less effective. Mary Death ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    Making aspirin solutions: Measure out 30ml of distilled water using a syringe and add the appropriate number of soluble aspirin tablets (see table2). Use a glass stirring rod to ensure the solution is mixed properly. Table 2. mixtures for aspirin solutions Aspirin tablets Total Aspirin (mg)

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    2 47.8 0.9 47.9 0.6 47.9 0.8 4 45.5 3.2 45.2 3.3 45.4 3.3 6 45.3 3.4 44.6 3.9 45 3.7 8 44.8 3.9 43.9 4.6 44.4 4.3 10 44.5 4.2 43.1 5.4 44 4.8 Average Amount: 42.9 2.8 Percentage of Catalase: 30% 1st Attempt 2nd Attempt Time: Initial Reading


    Boil some water in kettle then pour water into a large beaker (1000ml) 8. Add test tube labelled lipase and bile salt into beaker and monitor with thermometer until they reach 18�C. 9. Now add lipase and bile with the cream in the beaker labelled cream.

  2. Investigating the factors that affect the rate of hydrogen peroxide.

    Once the chemical bonds are broken, the complex is no longer able to fit in the active site, and breaks up. The enzyme is free to take part in the next reaction. Possible variables & Fair test Below are the possible variables in the investigation, how they will affect the

  1. Catalase activity in the liver

    The things I have to control in this experiment are as follows- When the reaction is taking place for each experiment I will measure the height of froth from the top of the mixture level therefore each experiment will be measured from the same place in all of the test tubes.

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    Different potatoes will not have exactly the same water and catalase content. Arefin Khan The mass will be kept constant in the same way as surface area and concentration of enzyme. Prediction: The higher the temperature, the higher the rate of reaction up to a certain point.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work