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Investigating the Rate of the enzyme Catalase in breaking down Hydrogen Peroxide.

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Introduction

Investigating the Rate of the enzyme Catalase in breaking down Hydrogen Peroxide Planning In this experiment I will be investing the effect on temperature on the enzyme Catalase when breaking down Hydrogen Peroxide. An enzyme is a biological catalyst, which speeds up a reaction, without itself being used up. It works a bit like a pair of scissors, by cutting up the molecule into smaller, simpler parts. The enzyme of Catalase is found in every living cell and breaks down Hydrogen Peroxide into water and oxygen. This is very important because Hydrogen Peroxide is very dangerous (it is more commonly known as Bleach). It is an irritant and it is produced in every living cell, and can do serious damage if it is not broken down by Catalase. Because normal living processes produce Hydrogen Peroxide, Catalase needs to be present to break it down quickly, and so Catalase can also be found in every living cell. Here is a reaction diagram to show the reaction of Hydrogen Peroxide and Catalase: Hydrogen Peroxide + Catalase Water + Oxygen This diagram shows that when Hydrogen Peroxide reacts with Catalase it produces Water and Oxygen, which are a lot less dangerous than Hydrogen Peroxide. ...read more.

Middle

I think this because, as I have mentioned earlier, the enzyme of Catalase is present in every living cell, which includes those of animals, and if the enzyme is present in a human then it will be best suited to this temperature. If the enzyme is too cold or too hot, then the enzyme can become denatured and not work properly, which could ultimately lead in the living thing dying. The closer the Hydrogen Peroxide is to 36-37�C, the better the enzyme will work and the more oxygen is produced. If it is too cold, then it will not work as well, and also if it is too hot. Obtaining Temperature of Hydrogen Peroxide (�C) Amount of Oxygen Collected (cm3) 20 4.2 30 10.8 40 8.4 50 5.4 60 2.5 Temperature of Hydrogen Peroxide (�C) Amount of Oxygen Collected (cm3) 20 4.0 30 3.5 40 7.0 50 3.0 60 1.1 Temperature of Hydrogen Peroxide (�C) Amount of Oxygen Collected (cm3) 20 5.0 30 8.5 40 8.5 50 6.8 60 1.2 Temperature of Hydrogen Peroxide (�C) Average Amount of Oxygen Collected (cm3) 20 4.4 30 7.6 40 8.0 50 5.1 60 1.6 Analysis I can see from my graphs that the 1st, 3rd and average follow the pattern that the closer the Hydrogen Peroxide is ...read more.

Conclusion

This means that only a tiny bit of oxygen could get through to the measuring cylinder, and this result is completely worthless. Other things which could have skewed the results is the length of the potato was quite hard to accurately measure, so there could have been less or more amounts of Catalase, as there was less/more potato. And, even touching the potato can kill cells, which can drastically lessen the amount of Catalase. Also, the temperature for taking the Hydrogen Peroxide off the heat to the time it takes to start the experiment may decrease, which can result in a large change in the reaction. If I was re-doing this experiment, I would probably use the same procedure as I did in this one, but I would like to be able to use more accurate measuring equipment, so to make the results more accurate, and hence, would mean more. On the whole, I thought that this experiment went well. The only thing that went really wrong was the second results, which only wrong because of no fault of my self, and it was the equipment that was wrong. I'd like to do this investigation again, because I could see whether my procedure was correct or not, and to see if my prediction, without any anomalous results skewing my averages. ...read more.

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