The variable I have chosen to investigate into is the concentration of the enzymes. This will mean that I will alter the concentration of catalase whilst using water to keep the volume the same overall. So, as I decrease the amount of catalase I will increase the amount of water. Hopefully, this will then mean that I will be able to easily see how the concentration of catalase affects the rate of decomposition.
Prediction
By using my scientific knowledge and the research into the characteristics of my variables, I predict that the more yeast there is (i.e. catalase enzymes), the more oxygen will be produced and the reaction will be quicker as a result of this. This is because, the larger the amount of catalase enzymes the more overall amount of particles to decompose and to break into the hydrogen peroxide. This means that the hydrogen peroxide will decompose at a slower rate, therefore producing more oxygen.
Apparatus
In order to carry out my experiment, I will need the following equipment:
- Conical Flask
- Gas syringe
- 10 test tubes
- Safety glasses
- Gas chamber
- Separator
- Test tube holder
- Metal clamp stand
- Stopclock
- Yeast (containing catalase)
- Hydrogen Peroxide
- Bung
Fair Test
In order to obtain reliable results, I will need to make sure that each of the tests I carry out will be equal and fair. There are three different variables which affect the rate of the reaction in this experiment : temperature,concentration of enzymes, and the level of hydrogen peroxide. There are a number of different factors within the environment of the experiment which could alter the rate of the reaction. So as to keep the experiment fair, I must keep certain aspects of the experiment the same, and alter only one component. I have chosen to alter the concentration of enzymes (i.e. the catalase within the yeast). This will consequently alter the amount of hydrogen peroxide . I will keep the temperature the same by completing all of the experiments within the same day, and in the same room. This means that I will be keeping the temperature consistent as far as is humanly possible. This is instead of completing the test over a number of days which would consequently have different temperatures, and therefore an effect on the rate of the reaction.
Method
I will use the apparatus listed above in order to carry out my experiment. Firstly, I will prepare myself for the experiment so that I will be safe. I will tie my hair back, make sure there are no hazards around the workplace such as bags or stools and then I will put on my safety goggles. Then, I will proceed to set up my equipment by fixing up the sand and clasping the gas chamber and the separator in it. The separator is used so that no liquid will get inside the gas chamber, as the syringe would then stick and the results would be unreliable. I will then connect the separator to the bung which I will place in to my test tubes when carrying out the experiment. Then I will use the different measurements of yeast and hydrogen peroxide and place these into the test tube whilst quickly putting the bung in and starting the stopclock. The measurements I will use are from 1-10cm3.I I will then taking a reading off the gas chamber after a number of 10 second intervals i.e. at 10,20 and 30 seconds. However, in my pilot tests I will only collect my results after 30seconds. These are my results. This method will also apply when carrying out my pilot tests, however I will not need to take as many readings. After I have collected all of my results, I will clear all of the apparatus away safely and make sure that it is all clean before putting it away.
Pilot Tests
In my pilot tests I will only need to take readings for 1, 5 and 10 cm cubed of catalase. I will only take a reading after 30 seconds however, as I only need to find out if my apparatus is working correctly and producing reliable results. I followed the method written above.
My results seem reliable as, they fit my prediction and there seem to be no anomalies or extreme pieces of data. The amount of oxygen produced ranges from 28 to 50 cm3, this means that there is a difference of 22cm3 between them. This seems reasonable, as, the highest amount of yeast i.e. 10cm3, produced the highest amount of oxygen i.e. 50cm3. The data for the smallest amount of yeast (1cm3) also seemed to be reasonable (28cm3).
Final Results
Graph One
Graph Two
Graph Three
Conclusion
By looking at the way in which my results are displayed in my graphs I can deduce that indeed, as I predicted, as time goes on more oxygen is produced. Graph one shows that there is quite a small amount of oxygen produced, however the steep gradient of the line of best fit tells us that the reaction occurred at quite a fast rate. This is backed up by the equation of the line of best fit which accurately shows us the rate of the reaction. Furthermore, graph two shows us that although there is a slightly larger amount of oxygen produced, the overall rate of the reaction slows down quite substantially as shown by the low sloping gradient of the line of best fit and its equation. As well as this, graph three shows that it also produces a very small amount more of oxygen, however the rate of the reaction also slows down considerably more than graph two. Overall, my results agreed perfectly with my prediction. I can tell this by looking at the amount of oxygen produced and the rate of the reaction over the different periods of time.
Evaluation
My results were seemingly very accurate, I can tell this by looking at my graphs as mostly they all fitted in a neat line with a strong line of best fit. So, because the data all has quite strong positive correlation, this shows that the amount of oxygen produced is directly proportional to the amount of yeast enzymes. However, in every graph I can see that the very first result I took was considerably lower than the rest of the results. Each graph has this result as its single anomalous result. This anomaly was most likely to have been caused by a piece of the equipment. Perhaps it was the syringe that took a while to start moving, this could have been caused by a small amount of water getting trapped in the syringe making it stick.
The only other thing in my method that could have caused unreliable or inaccurate data could be the fact that the timing of the experiment could not be absolutely accurate as I was using a stopclock to manually time it myself. Although, allowing for human error, I feel that my results, as shown, are overall very accurate. In order to improve my method in future I could use a data logger to time my results as this would be able to take a number of consistent more accurate readings. My graphs are clear and obvious, and give sufficient evidence to support my conclusion and prediction as everything in my method went as planned and I produced a set of plausible, accurate results.