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Investigation into the effect of pH change on catalase reacting with hydrogen peroxide.

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Introduction

Investigation into the effect of pH change on catalase reacting with hydrogen peroxide. Aim To investigate and chart the effect of pH change on catalase activity. Hypothesis I believe that the optimum pH for enzyme activity will be a neutral or close to neutral one. I predict that it will be in the range of 5-9 on the pH scale. Background Information This experiment is very similar to the previous investigation with the only difference being that our variable in this experiment is the pH of the solution that the enzymes are in, rather than the temperature that the enzymes had to react at. The symbol pH refers to the concentration of hydrogen ions present in a solution. The greater the number of hydrogen ions present, the lower the pH and the more acidic the solution. ...read more.

Middle

5 samples of potato tissue were taken with the cork borer, and then these were measured to 1.5cm each. 5 test tubes were labelled -one for each of the pH solutions used - 2, 4, 6, 8 and 10. The potato tissue samples were then placed in the test tubes, with 5cm� of hydrogen peroxide 10 drops of detergent. The pH solutions were then added to their respective test tubes. The test tubes were then left in the test tube rack for five minutes while the enzymes reacted. After five minutes the level of froth at the top of the test tubes was measured using the ruler and then recorded. Results Solution pH Froth Height (mm) 2 7 4 11 6 17 8 25 10 25 Conclusion From our results, we can conclude that the optimum pH for catalase to function is a high, and more alkaline pH. ...read more.

Conclusion

Any error that occurred would have completely skewed our results, because there weren't even any repetitions of this experiment. Each pH was used only once, and this could have made our results invalid. More repetitions were definitely needed to make this experiment valid, because a single freak results would have altered our whole conclusion. There was also the possibility of the wrong volume of hydrogen peroxide, detergent, or the pH solution being added to the tissue, so perhaps more careful measuring is needed. Our range of pH solutions was fairly wide so that does not really need to be improved, but since our results showed that pH8 and pH10 were the optimum pH levels for catalase activity, another test tube with pH9 used would have helped us to discover if that was the optimal pH. This would have made our results more accurate and we could perhaps have found the single best pH for catalase activity. ...read more.

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