Investigation into the effect of salt solutions on plant cells

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Investigation into the effect of salt solutions on plant cells

Background

Osmosis is defined as:

"the net movement of water molecules from a less concentrated solution to a more concentrated solution across a semi-permeable membrane..."

This movement must take place across a partially permeable membrane such as a cell wall, which lets smaller molecules such as water through but does not allow bigger molecules to pass through. The molecules will continue to pass through until the area in which the molecules are found reaches a state of equilibrium, meaning that the molecules are randomly distributed throughout an object, with no area having a higher or lower concentration than any other.

Information on plant cells: Plant cells always have a strong cell wall surrounding them. When they take up water by osmosis they start to swell, but the cell wall prevents them from bursting. Plant cells become turgid when they are put in dilute solutions. The pressure inside the cell rises and eventually the internal pressure of the cell is so high that no more water can enter the cell. This liquid or hydrostatic pressure works against osmosis. Turgidity is very important to plants because this is what makes the green parts of the plant "stand up" into the sunlight.
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When plant cells are placed in concentrated sugar/salt solutions they lose water by osmosis and they become flaccid. This is the exact opposite of turgid. The content of the potato cells shrinks and pulls away from the cell wall. These cells are said to be plasmolysed. When plant cells are placed in a solution, which has exactly the same osmotic strength as the cells they are in a state between turgidity and flaccidity. We call this incipient plasmolysis.

Method

Step 1

* I will take 5 test tubes.

Test tube

Trace ...

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