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Investigation into the effect of temperature on catalase reacting with hydrogen peroxide.

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Introduction

Investigation into the effect of temperature on catalase reacting with hydrogen peroxide Aim To investigate and chart the effect of temperature on catalase reacting with hydrogen peroxide (H202). Hypothesis I believe that since enzymes usually function at their optimum level between 5 and 40�C the level of froth will be highest in the 20�C and 40�C beakers as the catalase enzymes will function best at these temperatures. Background Information Hydrogen peroxide is a chemical that breaks down into water and oxygen as shown: - hydrogen peroxide (2H2O2) � water + oxygen (2H2O) (O2) Catalase is an enzyme that promotes the breakdown of hydrogen peroxide into oxygen and water as in the equation: - catalase hydrogen peroxide water + oxygen One molecule of catalase can break down 40 million molecules of hydrogen peroxide each second. In this experiment the oxygen is released as bubbles which are gathered and trapped as froth by adding 10 drops of detergent into the hydrogen peroxide solution. ...read more.

Middle

The results were all recorded. Results Temperature (�C) Replicate Height of foam (cm) Ice - approx. 2 1 3.8 Ice - approx. 2 2 4.6 Room temperature 1 7.2 Room temperature 2 6.9 40 1 6.4 40 2 5.9 60 1 1.9 60 2 1.7 Ice Room 40�C 60�C Temp. Conclusion From our results, we can conclude that the optimum temperature for catalase activity with hydrogen peroxide is room temperature, which is between 20-23�C. Under these conditions, the height of foam was 7.2cm, then 6.9cm (an average of 7.05cm). This shows that catalase functions well at this temperature and reacts rapidly. At the temperature of ice (around 2�C) the height of foam was 3.8cm and 4.6cm (average 4.2cm). This is because the temperature of ice is below the main working range of enzymes (around 5-40�C) so the catalase cannot break down the hydrogen peroxide properly. At 40�C, the heights of foam were 6.4cm and 5.9cm (average 6.15cm). ...read more.

Conclusion

If too much detergent had been added there would have been a greater level of froth making it appear as though the enzymes had reacted more rapidly, when that would not have been the case. Another possible improvement to the experiment would have been to add more repetitions - two were not really enough to be certain to be sure of the results. One freak result would have completely skewed our results. Adding more would have enabled us to disregard a freak result. We could also have increased the range of temperatures to help us gather a wider range of results. This would have made it possible for us to find the optimum level of catalase activity more accurately and gauge it more finely. Since our two best temperatures for enzyme activity were 20�C and 40�C another temperature between these two (presumably 30�C) would have helped us to obtain the optimal temperature more precisely and it would have made our results more conclusive. Apart from these possible improvements this investigation was carried out well. ...read more.

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