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Investigation into the effects of trypsin on dried milk solution

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Introduction

Investigation into the effects of trypsin on dried milk solution For this investigation we are only required to carry out the analysis and evaluation. We formulated our own hypotheses. The teacher carried out the experiment and provided us with the results. Aim: To investigate whether changing the concentration of trypsin affects the rate of which the proteins in milk are broken down. Hypothesis: My hypothesis is that the higher the concentration of enzyme the faster the proteins in the dried milk solution will be broken down. I expect my results graph to look like: I expect this because if the enzyme is more concentrated then there are more particles of the enzymes to collide with the substrate molecules and change them into products. Results Here are the results in table format that we were given by our teacher: 1st Results 2nd Results Mean Results Enzyme % Time (s) Enzyme % Time (s) ...read more.

Middle

However, the speed of increase got slower and slower and was no longer directly proportional for example when the concentration was 1.5% the rate of reaction was 0.014054814 (1/seconds) but when the concentration was 3% the rate of reaction had not doubled it was 0.017574692 (1/seconds). The reason that it was no longer directly proportional is because eventually the concentration of the enzyme was so high that most of the substrate molecules were attached to a enzyme, because of this it was hard for the a enzyme that is not currently reacting with a substrate molecule to find a free substrate molecule. This will slow down the increase in the rate of the reaction. The way that enzymes work is sometimes called the "lock and key method". The enzyme is the lock; it is structured in such a way that there is an indentation on their surface. This indentation is called the active site. ...read more.

Conclusion

To make it possible to read the level of the milk you can either use a measuring cylinder with black lines which is not a good idea as a measuring cylinder is not a very accurate measuring device so I would use a pipette which is a far more accurate measuring device. To solve the problem of when the milk is clear a colorimeter can be used to measure the colour of the solution and a certain reading can be decided on as clear that way the solution will have the same level of transparency each time. I would also take a larger range of readings to determine what happened at the end of the curve. I would start at 0 and increase the enzyme concentration by .2% each time from 0 up to 6%. This would allow me to determine what happens at either end of the graph. Jo Skelker Biology coursework Page 1 of 4 ...read more.

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