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Investigation of reaction rates between Hydrogen Peroxide and Catalase.

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Jimmy Hamilton VM Biology Coursework Investigation. Investigation of Reaction Rates Between Hydrogen Peroxide and Catalase. Aim: To investigate the effect of enzyme concentration on the rate of reaction. Information: An enzyme is a biological catalyst found in all living organisms and controls all the chemical reactions that occur. A catalyst is a substance that speeds up a reaction, so enzymes speed up reactions in living organism. Enzymes are found in all living cells and here they are called intracellular enzymes. Enzymes are also found in the digestive system, where they are called extracellular enzymes. Thousands of reactions occur simultaneously in a living organism, each of which is controlled by an enzyme. All enzymes share common properties: 1) The act on a particular substance called the substrate, and will only act on this one substance, i.e. the are specific. For example; maltase (enzyme) acts on maltose(substrate) to make glucose (product.) 2) They can work in two directions, for example, glucose can be turned back into maltose using the enzyme maltase. In this instance, glucose is the substrate. The direction of the reaction depends on the quantities of substrate and product. If there is more maltose than glucose, then maltase will break down maltose to form glucose, but if there is more glucose, then glucose becomes maltose. 3) If the amount of enzyme is greater than the amount of substrate, the reaction will be faster. ...read more.


Ensure no water escapes, as the cylinder must be completely full of water in order to gain accurate results. 3) Connect the hose and glass tubing to the conical flask and place the end of the glass tube under the measuring cylinder. 4) Place a given amount of potato cubes in the flask and prepare the hydrogen peroxide. 5) Mix the reactants and start timing, noting readings from the conical flask at set time intervals, e.g. every 30 seconds. It is very important to get the bung on to the conical flask quickly as without it gas will escape and results will be inaccurate. 6) When the reaction ends, clean the equipment and repeat changing only the amount of potato cubes. Safety: Wear safety goggles as hydrogen peroxide is an oxidising agent. Should any peroxide be spilt clear it up immediately. To lessen the chance of this happening, always replace the lids of containers. In case of contact with skin, wash immediately to prevent irritation. To prevent damage to clothes by the peroxide, wear a lab coat. Take care when handling a scalpel as they are very sharp. Results. Surface area of potato 30cm2 in 80cm3 peroxide. Time (seconds) Volume of Gas (cm3) 0 0 30 5 60 10 90 14 120 17 150 21 180 24 210 27 240 30 270 32 300 34 330 36 360 39 390 42 420 45 450 48 480 50 510 52 540 54 570 55 600 57 Time (seconds) ...read more.


This was done so any anomalous results could be seen and noted. The third experiment was not repeated because the previous two experiments had proved my prediction, and the third experiment was close enough to the true values to reinforce this. The results could have been improved by making the following improvements: 1) There was a time delay in mixing the potato and hydrogen peroxide, which would have allowed gas to escape the Buchner flask and this could have affected the results. 2) The measuring cylinders used for this experiment had a scale which started at 24cm3. this meant that results below 24cm3 were estimated 3) I would need to find a way of stopping the reaction while I took the results. This could be done by kinking the delivery tube, so no more gas enters the measuring cylinder while a reading id being taken. Improvements: 1) The experiments could be run for longer. The reactions had not finished in my experiment, which lasted for 10 minutes. In my prediction I stated that the graphs would be curve shaped, but would gradually flatten as the reaction progressed. In order to prove this, I would need to run the experiments until the reaction finished. In my experiment, it was impractical to do this because it requires large amounts of time. 2) As an extension to the experiment, it would be useful to test other sources of catalase for example liver. To obtain true values for the reaction between catalase and hydrogen peroxide, a pure sample of catalase could also be used. ...read more.

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