• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
  1. 1
  2. 2
  3. 3
  4. 4
  5. 5
  6. 6
  7. 7
  8. 8
  9. 9
  10. 10
  11. 11
  12. 12
  13. 13

Investigation of reaction rates between Hydrogen Peroxide and Catalase.

Extracts from this document...


Jimmy Hamilton VM Biology Coursework Investigation. Investigation of Reaction Rates Between Hydrogen Peroxide and Catalase. Aim: To investigate the effect of enzyme concentration on the rate of reaction. Information: An enzyme is a biological catalyst found in all living organisms and controls all the chemical reactions that occur. A catalyst is a substance that speeds up a reaction, so enzymes speed up reactions in living organism. Enzymes are found in all living cells and here they are called intracellular enzymes. Enzymes are also found in the digestive system, where they are called extracellular enzymes. Thousands of reactions occur simultaneously in a living organism, each of which is controlled by an enzyme. All enzymes share common properties: 1) The act on a particular substance called the substrate, and will only act on this one substance, i.e. the are specific. For example; maltase (enzyme) acts on maltose(substrate) to make glucose (product.) 2) They can work in two directions, for example, glucose can be turned back into maltose using the enzyme maltase. In this instance, glucose is the substrate. The direction of the reaction depends on the quantities of substrate and product. If there is more maltose than glucose, then maltase will break down maltose to form glucose, but if there is more glucose, then glucose becomes maltose. 3) If the amount of enzyme is greater than the amount of substrate, the reaction will be faster. ...read more.


Ensure no water escapes, as the cylinder must be completely full of water in order to gain accurate results. 3) Connect the hose and glass tubing to the conical flask and place the end of the glass tube under the measuring cylinder. 4) Place a given amount of potato cubes in the flask and prepare the hydrogen peroxide. 5) Mix the reactants and start timing, noting readings from the conical flask at set time intervals, e.g. every 30 seconds. It is very important to get the bung on to the conical flask quickly as without it gas will escape and results will be inaccurate. 6) When the reaction ends, clean the equipment and repeat changing only the amount of potato cubes. Safety: Wear safety goggles as hydrogen peroxide is an oxidising agent. Should any peroxide be spilt clear it up immediately. To lessen the chance of this happening, always replace the lids of containers. In case of contact with skin, wash immediately to prevent irritation. To prevent damage to clothes by the peroxide, wear a lab coat. Take care when handling a scalpel as they are very sharp. Results. Surface area of potato 30cm2 in 80cm3 peroxide. Time (seconds) Volume of Gas (cm3) 0 0 30 5 60 10 90 14 120 17 150 21 180 24 210 27 240 30 270 32 300 34 330 36 360 39 390 42 420 45 450 48 480 50 510 52 540 54 570 55 600 57 Time (seconds) ...read more.


This was done so any anomalous results could be seen and noted. The third experiment was not repeated because the previous two experiments had proved my prediction, and the third experiment was close enough to the true values to reinforce this. The results could have been improved by making the following improvements: 1) There was a time delay in mixing the potato and hydrogen peroxide, which would have allowed gas to escape the Buchner flask and this could have affected the results. 2) The measuring cylinders used for this experiment had a scale which started at 24cm3. this meant that results below 24cm3 were estimated 3) I would need to find a way of stopping the reaction while I took the results. This could be done by kinking the delivery tube, so no more gas enters the measuring cylinder while a reading id being taken. Improvements: 1) The experiments could be run for longer. The reactions had not finished in my experiment, which lasted for 10 minutes. In my prediction I stated that the graphs would be curve shaped, but would gradually flatten as the reaction progressed. In order to prove this, I would need to run the experiments until the reaction finished. In my experiment, it was impractical to do this because it requires large amounts of time. 2) As an extension to the experiment, it would be useful to test other sources of catalase for example liver. To obtain true values for the reaction between catalase and hydrogen peroxide, a pure sample of catalase could also be used. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    The aim of this investigation is to find out what effect pH has on ...

    4 star(s)

    the conical flask with a spoon and immediately close the flask with the bung and while doing this attach the other end of the delivery tube to the pressure sensor * As soon as you've put the tube in the pressure sensor start the clock * Hold the syringe while

  2. Osmosis investigation

    Firstly, I should have been more careful and precise when cutting and measuring potato chips. Limited time and a small range of equipment- cork borers, ruler and a scalpel- led to the chips not being exact in size as lining them all up was a very time consuming task and I had to move on to making my solutions.

  1. For my coursework I will be performing an investigation into an experiment using hydrogen ...

    This is because proteins have a 3D shape. In our case the Catalase has a certain shape that the substrate fits into. At high temperatures the active site on the enzyme is altered, see diagram below. (Diagram showing active site on the enzyme is altered therefore stopping products being formed)

  2. For my coursework I had to study enzymes (catalysts) and the rate of reaction ...

    outliers as we cannot guarantee the performance of the equipment and the variables surrounding it. I also predict that the Preliminary test will go in my favour and support my hypothesis. This will then be further supported hopefully, by the reliability of my graph.

  1. Enzyme Investigation. How to find the best concentration of hydrogen peroxide that will react ...

    we changed these by testing different pH levels but kept the volume of it the same keeping a fair reliable test and accurate results. * 2 Measuring cylinders- to measure the substances accurately to the nearest cm� * Retort stand- to clamp the clamps to the gas syringe Method: 1.

  2. Biology catalyst couursework

    This happened because there will be half the reactant particles per unit volume then the probability of reactant particles colliding with each other will decrease by 50%. Overall I must look back briefly at the collision theory to show why the amount of oxygen produced decreased when the concentration did

  1. Investigating an enzyme-catalyzed reaction

    now had to make sure I knew how I could control my other variables to make sure that I got accurate and fair results. If I didn't control the variables I would get inaccurate results because the other variables may be changing through each different solution.

  2. Structures and functions in living organisms. Revision Notes

    It could cause cancer. Smoking causes cells to divide 2.48 Describe a simple experiment to investigate the effect of exercise on breathing in humans. First choose a sample of people and measure their breathing rates at rest. Then make them vigorously exercise for two minutes and measure their breathing rates again 2.49 Understand why

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work