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Investigation of the effect of glucose concentration on anaerobic respiration in yeast.

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Introduction

Aim: To investigate the effect of glucose concentration on anaerobic respiration in yeast. Method: I am going to do 5 tests & each test will be performed 3 times to get an average result. In one conical flask, I will mix together 25ml of yeast & 25ml of a glucose solution, with a delivery tube with a bung in the top of it and leave it for 5 min so it gets acclimatised; in the other conical flask I will put enough water to cover the end of the delivery tube. On the first test I will use a 10% concentration of glucose. On the second test I will use a 15% concentration of glucose. On the third test I will use a 20% concentration of glucose. On the fourth test I will use a 25% concentration of glucose. On the fifth test I will use a 30% concentration of glucose. I will then put the yeast & glucose mixture in a warm water bath and count how many bubbles of CO2 are produced within 5 min. ...read more.

Middle

Results Table: Test Number 10% Glucose concentration 15% Glucose concentration 20% Glucose concentration 25% Glucose concentration 30% Glucose concentration Test 1 28 bubbles in 5 min 33 bubbles in 5 min 54 bubbles in 5 min 61 bubbles in 5 min 107 bubbles in 5 min Test2 26 bubbles in 5 min 40 bubbles in 5 min 44 bubbles in 5 min 50 bubbles in 5 min 103 bubbles in 5 min Test3 27 bubbles in 5 min 45 bubbles in 5 min 56 bubbles in 5 min 65 bubbles in 5 min 100 bubbles in 5 min Average 27 bubbles in 5 min 39 bubbles in 5 min 51 bubbles in 5 min 59 bubbles in 5 min 104 bubbles in 5 min Safety Points: 1. Make sure all glassware is away from edge of table. 2. Put all Coats, Bags & Stools away. 3. Make sure water bath doesn't get too hot. 4. Always wash hands when finish. 5. Take care of all the glassware. Analysis: From my final results table, I can use the average results to plot a graph of results. ...read more.

Conclusion

They seem to be in the same sort of shape on my graph. However, on my repeats there are some noticeable differences between test 2 and the others. On test 2 for 20%, the result is 10 and 12 bubbles less than test 1 & 3 and this happens again for 25%, it is 11 and 15 bubbles less than test 1 & 3. The explanation I can offer for this is that there must have been a mistake I have made or I might have not let it rest for the allotted time. I thought all of my other results generally fitted the overall trend well. I thought the experiment allowed the prediction to be tested very well as it proved the points that I wanted it to, and very conclusively as well. I thought my method was pretty foolproof too which would help anyone who wanted to do this experiment and only had my piece of coursework. I think my results were reliable enough to support the prediction especially due to the fact that there are no obvious abnormalities. If I was to do the experiment again, I would use a different means of counting the bubbles as the human eye could make mistakes. ...read more.

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