• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11

Investigation of the effect of temperature on the action of peroxidase enzyme to decompose hydrogen peroxide

Extracts from this document...

Introduction

Investigation of the effect of temperature on the action of peroxidase enzyme to decompose hydrogen peroxide Aim To find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide. Planning I am measuring the amount of gas given off in the reaction. By collecting this figure I can determine the rate of reaction. If I know the rate of reaction I can find out what temperature peroxidase works best at. Equipment Variables Change The temperature of the reactants: 60 50 40 30 20 10 Control Amount of peroxidase (10ml) Amount of hydrogen peroxide (20ml) Length of experiment (2min) Time between measurements (15sec) Equipment used Safety I will keep my safety goggles on at all times to prevent painful eye injury. Method For accuracy I am using six different temperatures and I will repeat the whole experiment twice. ...read more.

Middle

Prediction My prediction is that peroxidase will work best at body temperature. In my preliminary experiments I discovered this reaction doesn't coincide with the collision theory. I found this because if you heat up the reactants the reaction is slower. Using the theory that enzymes are sensitive to temperature, cooling the enzymes slows down the reaction. When they warm up again they continue to work at their normal speed. Over heating permanently damages the enzymes because they change shape or become denatured. As the temperature rises, so does enzyme activity - until the enzyme becomes denatured then its activity falls suddenly to a zero. 15 30 45 60 75 90 105 120 60 20 40 43 70 75 80 85 90 50 50 70 80 110 130 130 135 135 40 80 110 120 130 180 190 200 200 30 20 25 30 35 70 75 80 85 20 20 22 25 29 30 33 37 41 10 25 30 40 45 48 50 58 ...read more.

Conclusion

they do not die. When you rise the temperature it makes the enzymes work harder and faster. This means my prediction was right. Evaluation Our results were a bit off but we didn't have any really bad ones, they fact that they weren't that good was because of the reactants being the wrong temperature. Or the gas not being read properly. Or the fact that there was already some air in the measuring cylinder. The experiment couldn't really have been improved but it could have been more efficient. Like we could have been more careful at reading the results and putting the water filled measuring cylinder in the trough. I think my results were correct because the look right on the graphs (there are no anomalous results) If I was to do this experiment again I would make the temperature band smaller in order to find the temperature at which the enzymes are denatured , something like between 34 and 43 degrees. This is because my graphs suggest that it be between those to temperatures at which the enzymes denature. Tom Stone 1 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. The effect of aspirin on the action of bovine liver catalase

    The reason that this occurred is probably because there needed to be some pressure to make the bead move, so pressure had to build up and then would be released in one go. This is likely to be caused by surface tension in the bead, which made of a small drop of water.

  2. Factors Affecting Enzyme Activity

    Also, after conducting these experiments I have decided to use a 1cm3 syringe as this will give me accurate results and I have decided to use a gas cylinder rather than a measuring cylinder to collect gas as this is more accurate and easy to read.

  1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    The substrate (milk) is held in the active site by temporary bonds forming a structure, which is referred to as the enzyme-substrate complex. The energy needed to allow such a reaction to continue, is called the activation energy. Indeed, to increase the rate of reaction and the formation of new

  2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    Has nozzle at the tip. Shaped for firm grip (simply squeeze to release the pure water). Cleaner than tap water. Allows user to manipulate the amount of water needed through the nozzle. As it is cleaner than the tap water, it minimises the impurities that can be mixed with the chemicals.

  1. Catalase activity in the liver

    for 30?C 1 135 2 100 3 125 4 125 5 100 6 95 7 125 Average 115 No test Amount of Froth (mm) for 40?C 1 135 2 132 3 136 4 131 5 127 6 112 7 122 Average 129 No test Amount of Froth (mm)

  2. Biology Foundation - Effect of Temperature on the Action of an Enzyme As a ...

    Increased kinetic energy of both the substrate and the enzyme molecules is brought about by increasing the temperature to about 40o C. This in effect means the effect of temperature T on the rate of reaction can be expressed by the temperature coefficient Q10: Q10 = rate of reaction at

  1. Investigate the action of the Enzyme Catalyse

    Mass: The mass of potato will always be the same. It will be as accurate as possible and I will use the weighing scales to do this. Amount of H2O2: The amount of Hydrogen Peroxide will always be the same.

  2. The action of enzymes.

    I am saying this because the optimum level of the enzyme is roughly 40�c, anything above 45�c and the enzyme will start to denature and not work properly causing the rate of reaction to decrease. Also if the temperature is below 40�c then the enzyme will work too slow due to temperature.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work