Investigation of the effect of temperature on the action of peroxidase enzyme to decompose hydrogen peroxide

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Investigation of the effect of temperature on the action of peroxidase enzyme to decompose hydrogen peroxide

Aim

To find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide.

Planning

I am measuring the amount of gas given off in the reaction. By collecting this figure I can determine the rate of reaction. If I know the rate of reaction I can find out what temperature peroxidase works best at.

Equipment

Variables

Change

The temperature of the reactants:

60

50

40

30

20

10

Control

Amount of peroxidase (10ml)

Amount of hydrogen peroxide (20ml)

Length of experiment (2min)

Time between measurements (15sec)

Equipment used

Safety

I will keep my safety goggles on at all times to prevent painful eye injury.

Method

For accuracy I am using six different temperatures and I will repeat the whole experiment twice.

I will record