Part of The Student Room Group

  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10
  11. 11
    11
    • View my saved documents

    Investigation of the effect of temperature on the action of peroxidase enzyme to decompose hydrogen peroxide

    Extracts from this document...

    Introduction

    Investigation of the effect of temperature on the action of peroxidase enzyme to decompose hydrogen peroxide Aim To find the effect of temperature on an enzyme in this case peroxidase, by studying it decomposing hydrogen peroxide. Planning I am measuring the amount of gas given off in the reaction. By collecting this figure I can determine the rate of reaction. If I know the rate of reaction I can find out what temperature peroxidase works best at. Equipment Variables Change The temperature of the reactants: 60 50 40 30 20 10 Control Amount of peroxidase (10ml) Amount of hydrogen peroxide (20ml) Length of experiment (2min) Time between measurements (15sec) Equipment used Safety I will keep my safety goggles on at all times to prevent painful eye injury. Method For accuracy I am using six different temperatures and I will repeat the whole experiment twice. ...read more.

    Middle

    Prediction My prediction is that peroxidase will work best at body temperature. In my preliminary experiments I discovered this reaction doesn't coincide with the collision theory. I found this because if you heat up the reactants the reaction is slower. Using the theory that enzymes are sensitive to temperature, cooling the enzymes slows down the reaction. When they warm up again they continue to work at their normal speed. Over heating permanently damages the enzymes because they change shape or become denatured. As the temperature rises, so does enzyme activity - until the enzyme becomes denatured then its activity falls suddenly to a zero. 15 30 45 60 75 90 105 120 60 20 40 43 70 75 80 85 90 50 50 70 80 110 130 130 135 135 40 80 110 120 130 180 190 200 200 30 20 25 30 35 70 75 80 85 20 20 22 25 29 30 33 37 41 10 25 30 40 45 48 50 58 ...read more.

    Conclusion

    they do not die. When you rise the temperature it makes the enzymes work harder and faster. This means my prediction was right. Evaluation Our results were a bit off but we didn't have any really bad ones, they fact that they weren't that good was because of the reactants being the wrong temperature. Or the gas not being read properly. Or the fact that there was already some air in the measuring cylinder. The experiment couldn't really have been improved but it could have been more efficient. Like we could have been more careful at reading the results and putting the water filled measuring cylinder in the trough. I think my results were correct because the look right on the graphs (there are no anomalous results) If I was to do this experiment again I would make the temperature band smaller in order to find the temperature at which the enzymes are denatured , something like between 34 and 43 degrees. This is because my graphs suggest that it be between those to temperatures at which the enzymes denature. Tom Stone 1 ...read more.

    The above preview is unformatted text

    This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

    Found what you're looking for?

    • Start learning 29% faster today
    • 150,000+ documents available
    • Just £6.99 a month

    or


    Not the one? Search for your essay title...
    • Join over 1.2 million students every month
    • Accelerate your learning by 29%
    • Unlimited access from just £6.99 per month

    See related essaysSee related essays

    Related GCSE Patterns of Behaviour essays

    1. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

      Variables There are a number of variables, which must be controlled at all times throughout the experiment to ensure that my results are reliable and accurate, in addition to clarifying a fair test was conducted. The controlled variables are presented in the table below: Controlled variables Reasons why it must

    2. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

      When temperature raises so does the energy between the atoms. Therefore; an increase in collisions. * An increase in enzymes means that the rate would increase as more and more enzymes collide with the substrates, faster and faster. * When more collisions occur, the new product is made much faster.

    1. The effect of aspirin on the action of bovine liver catalase

      beginning to overlap because the change in rate of reaction is slowing down, it may be that if higher concentrations of aspirin were used the graph would level off soon after this point. There have been no values that have been considered anomalous as all of the values for rate of reaction are within 0.1 mm3s-1 of their average.

    2. Factors Affecting Enzyme Activity

      I have chosen to use the concentration of the liver, fruit or vegetable suspension as my factor to investigate as it will be a relatively easy experiment to conduct and is easily controlled. Also, I think that out of my three main possible factors this will give me the best pattern of results to analyse.

    1. An investigation of a factor affecting the activity of Liver peroxidase enzyme

      To keep it in this position a retort-stand, clamp and boss were used. After 2 minutes, the liver was dropped in the boiling tube. Straight away a rubber bung connected to a delivery tube was put on the boiling tube.

    2. An investigation to look at the effect of temperature on enzyme activity

      Diagram Preliminary work I will carry out some preliminary work to find out the pH required for optimal pepsin activity. Variables: Changed variables - pH (0.5, 1, 1.5, 2 molar HCl) Controlled variables -Same equipment -Same temperature (40�c) -Same person timing -Same person judging when cross is clearly visible -Same

    1. Investigate the action of the Enzyme Catalyse

      This is so that the same amount of Hydrogen Peroxide is "attacking" the potato at once and it is fairer. Preliminary: During my preliminary experiment I tested the accuracy of my experiment and worked out the quantities needed for my actual experiment.

    2. Biology Foundation - Effect of Temperature on the Action of an Enzyme As a ...

      Increased kinetic energy of both the substrate and the enzyme molecules is brought about by increasing the temperature to about 40o C. This in effect means the effect of temperature T on the rate of reaction can be expressed by the temperature coefficient Q10: Q10 = rate of reaction at

    • Over 160,000 pieces
      of student written work
    • Annotated by
      experienced teachers
    • Ideas and feedback to
      improve your own work