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Investigation of the effect of temperature on the rate of a Lipase Reaction

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Introduction

Investigation of the effect of temperature on the rate of a Lipase Reaction I am going to look at the effect of temperature on the rate of a Lipase Reaction, and to look at this I will be changing the temperature variable. In order to measure this, I will be doing the following practical: 1) Add 5cm� of milk and 1cm� of Lipase solution at different temperatures. 2) Then I will measure the reaction every 30 seconds, from 30 seconds to 2 minutes with a pH probe (as lipase breaks down into fatty acids) to test the strength of the acid produced. This will show how well the enzyme has worked and a pattern should emerge in my results relating the temperature to the strength of the acid. The stronger the acid, the better the enzyme has worked. This will be shown in my results as the more under a pH of 7 the final pH is, the better the enzyme has worked during the reaction at a certain temperature. For example, a final pH of 6.1 and a final pH of 7.3 shows that the first pH is stronger and therefore more acid has been produced as the lipase have broken down into fatty acids. ...read more.

Middle

As acid is being produced, there are general safety considerations to consider and goggles will be worn at all times when acid is present. Also, basic classroom rules will be followed, such as no running, to again ensure the complete safety of all present. My reason for introducing steps to produce reliable evidence is simple: to be able to write an accurate and concise conclusion, all evident provided has to be reliable and accurate. Human error is unavoidable, but steps can be taken to reduce this as much as possible. I have chosen a pH probe as this is simply more accurate than humans and can do a more precise reading as it is computerised. I have also chosen now to use a temperature probe when checking the temperature of my water bath, as this is computerised and therefore would be expected to be more reliable than using a thermometer. This would also be quicker, as a thermometer would have to adapt from the rooms temperature before being able to reliably use it, whereas a probe would be immediately able to take an accurate temperature reading. ...read more.

Conclusion

I would then have used this information in order to predict the results. If I were to do this experiment again, this is what I would do as I would have had more idea scientifically about what to expect. I found I had no anomalous results and I believe this to be because I followed a strict procedure and was thoroughly conscientious at all times. I controlled variables accurately and checked and double checked readings. I used the same stopwatch throughout when timing and used all the same equipment, such as pH probes and temperature probes. I constantly monitored the temperature of my water baths, ensuring that they were always the exact and same temperature throughout each experiment. To provide an even firmer conclusion, if I were to perform this experiment again I would do a number of things. I would firstly use more temperatures, for example not just 20, 30, 40, 50 & 60�C but 20, 25, 50, 35, 40, 45, 50, 55, 60�C. This would provide more information as more results are being obtained on which to base a conclusion. I would also research the conditions of the pancreas more thoroughly so that I could predict scientifically what would happen using better knowledge. ...read more.

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