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Investigation of the effects of sucrose molarity on cells in the stem tuber of a potato.

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Introduction

Investigation of the effects of sucrose molarity on cells in the stem tuber of a potato Planning In this investigation I am trying to find out what molarity of sucrose solution is same as the molarity of sucrose in the cells of a potato. In this experiment I am going to change the molarity of the sucrose solution out the cells of potato. I predict that if the molarity of sucrose solution outside the cells is isotonic to the molarity of the sucrose inside the cells then there will be no change in weight of the potato disks. I predict this because if the molarities are isotonic inside and outside the cells then there will be a balanced osmotic movement of the water molecules from inside and outside hence there will be no change in weight. I also predict that if the molarity of the sucrose solution is hypertonic outside the cell than the inside then there will be loss in weight because osmosis is the movement of water molecules from an area of high concentration of water molecules to an area low concentration of water molecules and if there is there is a ...read more.

Middle

variables could affect the process of osmosis in the potato cells In this experiment use of the equipment is really important and it could affect the results I get. For example if I was supposed to use a blunt knife to cut the potato disks, there is a huge chance that I will rupture the cell membranes of the potato. The ruptured cells of the potato will not take part osmosis Therefore I will be very careful in cutting the disks. Below is the results table of my preliminary investigation. Molarity of sucrose solution (M) Weight of disks before (g) Weight of disks after (g) % change in weight 0 0.24 0.28 +16.67 0.24 0.28 +16.67 1 0.24 0.16 -33.3 0.24 0.15 -37.5 Now that I know that the disks of potato has gained weight in the 0 molar sucrose solution and they have lost weight in the 1 molar sucrose solution I have decided that my range of the molarity of the sucrose solution will be between 0 and 1 molar because I want to find out at which molarity of sucrose solution the potato's weight is unchanged. ...read more.

Conclusion

My conclusion does support my prediction where I say that if the concentration of the sucrose solution is hypertonic outside the cell there will be decrease in weight and vice versa if the concentration of the sucrose solution is hypotonic outside the cell there will increase in weight of the cell and if the tonicity of the solution is isotonic at both sides then there will be no change in weight and like wise my results support my prediction too Evaluation The procedure that I have used to obtain the results is quite straight forward yet accurate. I think the results I obtained are reliable and accurate as there are not many anomalous points in my plotted graph and they fit the pattern very closely. I think that this investigation was carried on fair grounds and I tried and kept the controllable variable constant. To support my conclusion I have collected enough evidence, I have collected three readings for each sucrose solution and I have got an average for them. If I was to repeat this experiment again I will use different types of potato for my investigation. This will help me in understanding the osmotic movement in different types of potato. - 1 - ...read more.

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