Investigation of the effects of sucrose molarity on cells in the stem tuber of a potato.

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Investigation of the effects of sucrose molarity on cells in the stem tuber of a potato

Planning

In this investigation I am trying to find out what molarity of sucrose solution is same as the molarity of sucrose in the cells of a potato. In this experiment I am going to change the molarity of the sucrose solution out the cells of potato.

I predict that if the molarity of sucrose solution outside the cells is isotonic to the molarity of the sucrose inside the cells then there will be no change in weight of the potato disks. I predict this because if the molarities are isotonic inside and outside the cells then there will be a balanced osmotic movement of the water molecules from inside and outside hence there will be no change in weight. I also predict that if the molarity of the sucrose solution is hypertonic outside the cell than the inside then there will be loss in weight because osmosis is the movement of water molecules from an area of high concentration of water molecules to an area low concentration of water molecules and if there is there is a higher concentration of sucrose solution outside then the water molecules will diffuse outside the cell, which suggests that there will be loss in weight. Vice versa if there is hypotonic concentration of sucrose solution outside the cells compared to inside then there will be gain in weight.

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Below is the method that I am going to follow through my preliminary investigation.

  1. I will take a potato and cut it into half
  2. Using a hole borer I will then take one cylinder of potato tissue from the potato.
  3. I will then cut the cylinder into four disks and I will then weigh them using an electronic balance. In order to get accurate weights I will weigh the filter paper and the cell sap on it with the potato disk ...

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