• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation On Enzymes And The Rate Of Reactions

Extracts from this document...


Investigation On Enzymes And The Rate Of Reactions Aim: The aim of this investigation is to see how surface area affects the rate of reaction. Introduction This investigation is about a potato being put into hydrogen peroxide. This investigation will allow me to find out about the enzyme- catalase, which is found in potato cells. Enzymes are protein molecules and act as catalysts within the cells. Catalysts help to speed up a reaction and is not itself used up during the reaction. The enzyme can be affected by the variables temperature and pH. If the temperature is too high the enzyme will denature meaning it will no longer work. Enzymes work to a specific temperature and work best at 40 C and will denature above 45 C. An enzyme also has an optimum pH and works best at about pH 6.5. Variation either side of pH results in denaturising of the enzyme and a slower rate of reaction. The temperature and pH affect of the enzyme can be shown on graphs: Temperature affect on enzyme: pH affect on enzyme: The enzyme is thought to be a particular shape. This shape allows two reacting molecules to come close together on the enzyme (The lock and key theory), which makes the reaction easier to carry out. ...read more.


Then cut 3 segments into their appropriate amount of pieces to get the different surface areas. (The first potato is left as it is, the second is cut into half, the third is cut into four pieces and the fourth is cut into five pieces.) 5)Drop each segment of potato, one by one, into the H O whilst counting every minute for 10 minutes 6)Measure the amount of froth produced at the end of each minute and record results. 7)Repeat the experiment again to get results which are to be used to compare the accuracy of each experiment. Obtaining Evidence I obtained evidence of the experiment accurately and safely which is adequate for the activity. Safety Precautions I ensured there was a safe working area. I also was careful whilst handling the chemical hydrogen peroxide as it is corrosive. To prevent an accident occurring I also took care whilst handling the core-borer and the knife. The data obtained from this investigation has been recorded in a table showing the height of froth (oxygen gas) after every minute for a period of ten minutes, of each of the four surface areas. The table also includes the results of the first and second experiment and also an average of both the experiments. ...read more.


have also affected some of the results because light breaks down the H O because light breaks down the H O resulting in the water particles separating from the H O , before the experiment has actually begun because no froth is made because the enzyme in the potato is needed. People in the group may have also measured the height of the froth at different times resulting in inaccurate results. My results could be more accurate if the above factors were taken into consideration. In this investigation I received most of the evidence needed from my plan but alterations could have been made to the experiment to get more precise results. I could have done each part of the experiment individually so that it is done by the same person and could have used the time more efficiently. To get even more accurate results I could have made more accurate measurements. My first set of results disagrees with the second set of results slightly, due to the anomalies in the first experiment. I did get enough evidence to make a firm conclusion, although some of the results were wrong. To get more evidence for the same factor, other than the mentioned improvements I could use a gas syringe to measure the gas (O ) released for a more precise result. Rupal Ladva Biology Coursework ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Investigating the effects of surface area on the rate of enzyme reactions.

    4 star(s)

    If the graph does eventually level out, it is due to there not being enough substrate to bind with all the free active sites there are. A higher concentration of enzymes after this "maximum point" will do nothing for the amount of gas evolved.

  2. Marked by a teacher

    Biology Coursework Enzymes

    4 star(s)

    fluids Filter paper 3+ Knife 1 Measuring Cylinder 3 Potato 1 Method In order for me to be able to investigate the effect of starch concentration on an amylase controlled reaction I will follow a method for me to be able to efficiently and effectively conduct my investigation.

  1. Marked by a teacher

    Biology Coursework: Investigation of How Ph Affects the Rate of Reaction of Catalase

    3 star(s)

    This is represented in the graph because it forms a similar 'dip and curve', seen in the 10ml experiment. * The dip is where the optimum pH range is for the catalase. * The curve is where the pH range is outside the optimum range and curving towards a longer

  2. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    * The measuring cylinder used to collect the oxygen released needs to be clear so that I can view the experiment properly and gain an accurate result to base a conclusion on. If all these factors are controlled it will make my test a fair one and also lead me to gain reliable results.

  1. Osmosis investigation

    I will be taking results at 0.2Molar intervals. From my preliminary I found that the 0.0Molar reading was valuable and important to include in my experiment as it shows the effect of osmosis very strongly as there is a very high water potential. For that reason, my concentration readings start at 0.0Molar- that is in fact tap water.

  2. Investigation on how pH affects free and immobilised catalase enzymes.

    OVERALL BUFFER 2 7 12 17 A and B 7 14 20 34 Distilled water 13 6 9 15 A and B 5 cm3 10vol hydrogen peroxide 2grams of liquefied potato Buffers A and B stand for citric acid and anhydrous disodium hydrogen phosphate.

  1. Osmosis Investigation

    On the other hand, the potato cell sap has a higher water potential because it has a weaker concentration, which means there are less solute molecules and thus, more freely moving water molecules. As a result, the potato cell sap acts as the hypotonic (dilute)

  2. For my coursework I had to study enzymes (catalysts) and the rate of reaction ...

    Aim My initial aim was to measure the volume of oxygen when the concentration of hydrogen peroxide is added to the enzyme (potato) and then subsequently decreased. Along with this I have many more aims which I wish to achieve they are as follows: - To identify the factors and variables and account for them.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work