• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation On Enzymes And The Rate Of Reactions

Extracts from this document...


Investigation On Enzymes And The Rate Of Reactions Aim: The aim of this investigation is to see how surface area affects the rate of reaction. Introduction This investigation is about a potato being put into hydrogen peroxide. This investigation will allow me to find out about the enzyme- catalase, which is found in potato cells. Enzymes are protein molecules and act as catalysts within the cells. Catalysts help to speed up a reaction and is not itself used up during the reaction. The enzyme can be affected by the variables temperature and pH. If the temperature is too high the enzyme will denature meaning it will no longer work. Enzymes work to a specific temperature and work best at 40 C and will denature above 45 C. An enzyme also has an optimum pH and works best at about pH 6.5. Variation either side of pH results in denaturising of the enzyme and a slower rate of reaction. The temperature and pH affect of the enzyme can be shown on graphs: Temperature affect on enzyme: pH affect on enzyme: The enzyme is thought to be a particular shape. This shape allows two reacting molecules to come close together on the enzyme (The lock and key theory), which makes the reaction easier to carry out. ...read more.


Then cut 3 segments into their appropriate amount of pieces to get the different surface areas. (The first potato is left as it is, the second is cut into half, the third is cut into four pieces and the fourth is cut into five pieces.) 5)Drop each segment of potato, one by one, into the H O whilst counting every minute for 10 minutes 6)Measure the amount of froth produced at the end of each minute and record results. 7)Repeat the experiment again to get results which are to be used to compare the accuracy of each experiment. Obtaining Evidence I obtained evidence of the experiment accurately and safely which is adequate for the activity. Safety Precautions I ensured there was a safe working area. I also was careful whilst handling the chemical hydrogen peroxide as it is corrosive. To prevent an accident occurring I also took care whilst handling the core-borer and the knife. The data obtained from this investigation has been recorded in a table showing the height of froth (oxygen gas) after every minute for a period of ten minutes, of each of the four surface areas. The table also includes the results of the first and second experiment and also an average of both the experiments. ...read more.


have also affected some of the results because light breaks down the H O because light breaks down the H O resulting in the water particles separating from the H O , before the experiment has actually begun because no froth is made because the enzyme in the potato is needed. People in the group may have also measured the height of the froth at different times resulting in inaccurate results. My results could be more accurate if the above factors were taken into consideration. In this investigation I received most of the evidence needed from my plan but alterations could have been made to the experiment to get more precise results. I could have done each part of the experiment individually so that it is done by the same person and could have used the time more efficiently. To get even more accurate results I could have made more accurate measurements. My first set of results disagrees with the second set of results slightly, due to the anomalies in the first experiment. I did get enough evidence to make a firm conclusion, although some of the results were wrong. To get more evidence for the same factor, other than the mentioned improvements I could use a gas syringe to measure the gas (O ) released for a more precise result. Rupal Ladva Biology Coursework ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Life Processes & Cells section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Life Processes & Cells essays

  1. Marked by a teacher

    Investigating the effects of surface area on the rate of enzyme reactions.

    4 star(s)

    As I had predicted, the rate of reaction did increase as the surface area increased. It also appears to level off which is what I explained in the prediction. "Enzyme concentration: at low enzyme concentration there is great competition for the active sites and the rate of reaction is low.

  2. Marked by a teacher

    Biology Coursework Enzymes

    4 star(s)

    Apparatus The apparatus that I will use to aid me in my experiment will be a; Apparatus Quantity Reason for choice Test tube 3 To for the potato tubules Test tube rack 1 To hold test tubes Borer 1 Create tubules Scale 1 Weigh tubules before and after immersion in

  1. Marked by a teacher

    Investigating the breakdown of hydrogen peroxide by the enzyme catalyse in potatoes.

    4 star(s)

    Also to further improve a 25cm3 measuring cylinder could have been used for more accurate results. Another way in which the accuracy of the results could have been improved would have been to record for an even shorter period of time - perhaps 90 seconds, and use the extra time

  2. Marked by a teacher

    Biology Coursework: Investigation of How Ph Affects the Rate of Reaction of Catalase

    3 star(s)

    This is unexpected because it would have been expected that the pH was to be more neutral, but according to the readings the pH of the potato was 6.4.

  1. Osmosis investigation

    -18x potato chips (20mm/2cm) -1x Scales (sensitivity to be 2 d.p.) -1x Cutting tile -1x Beaker with 150ml3 of water -1x Beaker with 150ml3 of sucrose solution -1x Pipette for water -1x Pipette for sucrose -1x Cork borer size 4 -1x Cork borer size 5 -1x Ruler -1x Results table

  2. An Investigation into the effect of surface area on the activity of catalase in ...

    Resulting in four surface areas being investigated, and all results collected for correlation in graphs (Refer to diagram below to reference of how to cut potato chips). Diagram Safety: * Care should be taken when handling the scalpel and cork borer, in order to avoid injury, as they are very sharp objects.

  1. For my coursework I had to study enzymes (catalysts) and the rate of reaction ...

    When using the knife whether we were carrying or cutting, we had to be really careful and passive as it could harm someone if used incorrectly. Method I began by implementing all the safety precautions such as clearing my area, wearing the safety equipment etc.

  2. Investagating the Action of the Enzyme Catalase On the Surface Area of a Potato.

    Catalase 2H2O2 O2 (g) + 2 H2O (l) Hydrogen peroxide oxygen + water The catalyst in this experiment, which is catalase, remains unchanged after this reaction. Catalysts also work by providing an easier route for the reaction to follow such as having lower activation energy. If the activation energy is lower, many more of the collisions will have the necessary minimum energy and so produce a reaction.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work