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Investigation On Enzymes And The Rate Of Reactions

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Introduction

Investigation On Enzymes And The Rate Of Reactions Aim: The aim of this investigation is to see how surface area affects the rate of reaction. Introduction This investigation is about a potato being put into hydrogen peroxide. This investigation will allow me to find out about the enzyme- catalase, which is found in potato cells. Enzymes are protein molecules and act as catalysts within the cells. Catalysts help to speed up a reaction and is not itself used up during the reaction. The enzyme can be affected by the variables temperature and pH. If the temperature is too high the enzyme will denature meaning it will no longer work. Enzymes work to a specific temperature and work best at 40 C and will denature above 45 C. An enzyme also has an optimum pH and works best at about pH 6.5. Variation either side of pH results in denaturising of the enzyme and a slower rate of reaction. The temperature and pH affect of the enzyme can be shown on graphs: Temperature affect on enzyme: pH affect on enzyme: The enzyme is thought to be a particular shape. This shape allows two reacting molecules to come close together on the enzyme (The lock and key theory), which makes the reaction easier to carry out. ...read more.

Middle

Then cut 3 segments into their appropriate amount of pieces to get the different surface areas. (The first potato is left as it is, the second is cut into half, the third is cut into four pieces and the fourth is cut into five pieces.) 5)Drop each segment of potato, one by one, into the H O whilst counting every minute for 10 minutes 6)Measure the amount of froth produced at the end of each minute and record results. 7)Repeat the experiment again to get results which are to be used to compare the accuracy of each experiment. Obtaining Evidence I obtained evidence of the experiment accurately and safely which is adequate for the activity. Safety Precautions I ensured there was a safe working area. I also was careful whilst handling the chemical hydrogen peroxide as it is corrosive. To prevent an accident occurring I also took care whilst handling the core-borer and the knife. The data obtained from this investigation has been recorded in a table showing the height of froth (oxygen gas) after every minute for a period of ten minutes, of each of the four surface areas. The table also includes the results of the first and second experiment and also an average of both the experiments. ...read more.

Conclusion

have also affected some of the results because light breaks down the H O because light breaks down the H O resulting in the water particles separating from the H O , before the experiment has actually begun because no froth is made because the enzyme in the potato is needed. People in the group may have also measured the height of the froth at different times resulting in inaccurate results. My results could be more accurate if the above factors were taken into consideration. In this investigation I received most of the evidence needed from my plan but alterations could have been made to the experiment to get more precise results. I could have done each part of the experiment individually so that it is done by the same person and could have used the time more efficiently. To get even more accurate results I could have made more accurate measurements. My first set of results disagrees with the second set of results slightly, due to the anomalies in the first experiment. I did get enough evidence to make a firm conclusion, although some of the results were wrong. To get more evidence for the same factor, other than the mentioned improvements I could use a gas syringe to measure the gas (O ) released for a more precise result. Rupal Ladva Biology Coursework ...read more.

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