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Investigation on the effect of temperature on enzyme reaction

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Investigation on the effect of temperature on enzyme reaction Introduction: In the experiment we will investigate the effect of temperature on the reaction of an enzyme trypsin. To carry out the investigation we will use the enzyme trypsin and a piece of film which contains 3 different layers of gelatin, plastic backing and a black photosensitive chemical layer. Prediction: For this investigation I predict that the rise in temperature will effect the time in which the enzyme reacts. If my prediction is correct the rise in temperature should make the enzyme trypsin react quicker with the tape. The gelatin in the film is made of protein and the enzyme trypsin will react with this protein causing the gelatin to disappear and the photosensitive chemical layer to be broken off. The rate of most chemical reactions increases as temperatures rise. I predict that when the temperature in the water baths used is higher then the rate of reactions between the enzyme and film will increase, and when the temperature is lowered then the reaction will be slower. I also predict that if the temperature of the water baths exceeds the optimum temperature, which is around 50�C+, the enzyme will become denatured and there will no longer be a reaction taking place. ...read more.


3. Put the film into the test tube. 4. Start the stopwatch when the reaction starts which should be when the film is in contact with the substance inside the test tube. 5. Stop stopwatch when the reaction is clearly complete. 6. Note down reaction time results into a table. 7. Repeat the above steps for each temperature twice so that the experiment is accurate. TRIAL RUN In order to make sure that the main experiment was carried out correctly a trial run was required. A trial run gives you the opportunity to check if you are doing everything right and see if any improvements could be made on the final experiment. Range The approximate range of the temperatures for the trial run will be: 18�c 35�c 50�c 70�c And The range of concentration will be: 0.1% and 0.5% which will be tested to see which gives ideal times Here are the results from the trial run: Concentration of trypsin (%) Temperature (�c) Time taken (minutes) 0.5 18 12.59 0.5 35 5.36 0.5 50 3.39 0.5 70 2.59 0.1 18 14.38 0.1 35 9.04 0.1 50 5.17 0.1 70 5.50 After completing the trial run and looking at the results found I am not totally satisfied with the way the trial run had gone and the methods used to collect results. ...read more.


Most of the results where close together but some flaws in the method effected the reaction times. Evaluation After the final experiment has been completed and the results recorded I am moderately satisfied with the experiment. There was a lot of space for improvement both method and apparatus wise. I think that the results would have been a lot more accurate if we had had more time to complete the experiments and record the results. The accuracy in the final experiment was a lot better than the accuracy during the trial run. The trial run helped us to stop and think of what needed to be changed or what needed to be kept in order to find out how to correctly carry out the final experiment with as accurately as possible. One thing that was a big problem was the temperature of the water baths which weren't very reliable and proved a problem keeping them the right temperature. During the experiment the reactions should have been tested between 2-3 times to get a better range of results and to show clearly that the results are accurate. ...read more.

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