• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Investigation on the effect of temperature on enzyme reaction

Extracts from this document...

Introduction

Investigation on the effect of temperature on enzyme reaction Introduction: In the experiment we will investigate the effect of temperature on the reaction of an enzyme trypsin. To carry out the investigation we will use the enzyme trypsin and a piece of film which contains 3 different layers of gelatin, plastic backing and a black photosensitive chemical layer. Prediction: For this investigation I predict that the rise in temperature will effect the time in which the enzyme reacts. If my prediction is correct the rise in temperature should make the enzyme trypsin react quicker with the tape. The gelatin in the film is made of protein and the enzyme trypsin will react with this protein causing the gelatin to disappear and the photosensitive chemical layer to be broken off. The rate of most chemical reactions increases as temperatures rise. I predict that when the temperature in the water baths used is higher then the rate of reactions between the enzyme and film will increase, and when the temperature is lowered then the reaction will be slower. I also predict that if the temperature of the water baths exceeds the optimum temperature, which is around 50�C+, the enzyme will become denatured and there will no longer be a reaction taking place. ...read more.

Middle

3. Put the film into the test tube. 4. Start the stopwatch when the reaction starts which should be when the film is in contact with the substance inside the test tube. 5. Stop stopwatch when the reaction is clearly complete. 6. Note down reaction time results into a table. 7. Repeat the above steps for each temperature twice so that the experiment is accurate. TRIAL RUN In order to make sure that the main experiment was carried out correctly a trial run was required. A trial run gives you the opportunity to check if you are doing everything right and see if any improvements could be made on the final experiment. Range The approximate range of the temperatures for the trial run will be: 18�c 35�c 50�c 70�c And The range of concentration will be: 0.1% and 0.5% which will be tested to see which gives ideal times Here are the results from the trial run: Concentration of trypsin (%) Temperature (�c) Time taken (minutes) 0.5 18 12.59 0.5 35 5.36 0.5 50 3.39 0.5 70 2.59 0.1 18 14.38 0.1 35 9.04 0.1 50 5.17 0.1 70 5.50 After completing the trial run and looking at the results found I am not totally satisfied with the way the trial run had gone and the methods used to collect results. ...read more.

Conclusion

Most of the results where close together but some flaws in the method effected the reaction times. Evaluation After the final experiment has been completed and the results recorded I am moderately satisfied with the experiment. There was a lot of space for improvement both method and apparatus wise. I think that the results would have been a lot more accurate if we had had more time to complete the experiments and record the results. The accuracy in the final experiment was a lot better than the accuracy during the trial run. The trial run helped us to stop and think of what needed to be changed or what needed to be kept in order to find out how to correctly carry out the final experiment with as accurately as possible. One thing that was a big problem was the temperature of the water baths which weren't very reliable and proved a problem keeping them the right temperature. During the experiment the reactions should have been tested between 2-3 times to get a better range of results and to show clearly that the results are accurate. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Free essay

    Rate Of Reacion

    The moment this happens the pressure and gas given off changes quickly, therefore harder to read. However you could change the surface area to test the rate of reaction, and what this means is to break the solids into smaller pieces meaning a chemical reaction has more particles to work

  2. Investigation into the digestion of milk by Trypsin.

    This will show when compared to the other set of results how the temperature at which the reaction occurs at affects the rate at which the reaction occurs. Results: Apparatus Water bath x 4, (at 20, 40 60 and 80 degrees), glass beaker, ice, test tubes, trypsin solution, (1%), milk solution, (4%), Pipette, thermometer, measuring syringe, (25 cm^3).

  1. Investigation On The Enzyme Trypsin

    experiment: Time taken for the trypsin to digest the gelatine, the trypsin having been incubated for varying amounts of time (preliminary): Incubation time Time taken for reaction (seconds) Average Time taken for reaction Of Trypsin (seconds) Reading 1 Reading 2 Reading 3 (Nearest second)

  2. THE EFFECT OF BILE SALT ON THE ACTION OF THE ENZYME LIPASE

    best temperature to use for the experiment, which will enable me with reliable and clear results. Enzymes work best at around 37�C; this is because the molecules become excited as they receive more heat, thereby more kinetic energy forcing them to collide more frequently with the substrate.

  1. Factors Affecting Enzyme Activity

    I will firstly take a sample of the solution in a test tube and universal indicator solution to it to in order to monitor the pH of the suspension. I will not add a buffer to control the pH as the chemicals in the buffer could interfere with the reaction and alter my results.

  2. 'Carry out an investigation to determine a factor affecting the rate of digestion of ...

    The surface of the film will be saturated; therefore by lifting it in and out will remove this and allow new enzymes to come into contact with the gelatine. Stirring causes this; and so I will keep all movement of the test tube during the experiment to a minimum.

  1. Investigate the rate of a catalysed reaction, when altering the temperature of the solution ...

    it did, then the active site of the amylase should be able to change it's shape and catalyse other reactions with a different substrate. PREDICTION I predict that at very low temperatures such as 10 c, the reaction will take place very slowly.

  2. Investigating the effect of enzyme concentration on the rate of an enzyme catalysed reaction.

    Each enzyme has an optimum temperature; this is where the rate of reaction is at its maximum. If the temperature is above the optimum, the rate of reaction starts to decrease as the active site can start to distort, causing no enzyme-substrate complexes and producing no product, as the substrate cannot bind.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work