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Investigation Showing Factors Effecting Enzyme Activity.

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Investigation Showing Factors Effecting Enzyme Activity The chemical hydrogen peroxide can be broken down into water and oxygen using an enzyme as a catalyst. In this investigation, I aim to demonstrate the enzyme activity will be affected by different variables. Planning Enzymes are biological catalysts, which change the speed of a reaction. By braking larger molecules into smaller molecules. Enzymes have an active site, which has a specific shape depending of the type of enzyme it is, so it can only react with a specific substance. In this investigation I am going to use potato as my enzyme because it has the same substances in it at the liver has. The rate at which enzymes work, depends on the temperature. If the temperature is too low the enzymes will become inactive, this will happen because the active sight will not be able to bond with the substance. If the temperature is too high the enzymes will become denatured, this will happen because the active shape will change shape and will not fit the substance. The rate at which enzymes work also depends on other factor, one being the PH level. What factor am I going to change? ...read more.


4. Heat the hydrogen peroxide to the temperature needed, on the Bunsen burner. 5. Add the potato too the hydrogen peroxide, place the rubber bung on and start the timer. 6. Stop the timer after 100cm of oxygen has been produced. I will use five different temperatures, and I will repeat each one three times. I will do this so that I can average out my results, to make them more accurate. I will then put all my results in to a table and from that I will draw a graph. Is it a fair test? It is very important that I keep this experiment a fair test, otherwise my results will come out totally wrong. To make sure my experiment is a fair test, I will make sure that only the property changing is the temperature and nothing else. I will do this by making sure that at the start of every test I measure the hydrogen peroxide and potato accurately. As if one of them is not the right measurement, I may get anomaly result. Prediction I predict that in this experiment, as the temperature increases so will the rate of reaction. I know this because from previous knowledge, I know that as the temperature increases so will the rate of reaction to a certain temperature. ...read more.


I can tell this from the graph, because at 40�c the amount of gas produced per second is the biggest amount produced in the graph. Then at 50�c, the enzymes are becoming denatured and slowing down. I can tell this from the graph, by the fact that between 40�c to 50�c, the rate of reaction drops so dramatically. I did not have enough time to do a test for 60�c, but from the results I have so far. I can tell that at 60�c, the enzymes would have become totally denatured. I can tell this by the way the graphs slope/line decreases very steeply after 40, so from this I would think it would continue on decreasing at a rate like this. The prediction I made has been proved correct, from the evidence in my table and in graph 2. In my prediction I said that as the temperature increased, so would the rate of reaction, but when the temperature reached about fifty, the reaction will dramatically slow down and even stop as the enzymes will become denatured. Although I did not have time to do any experiments over 50�c, I can see from graph 2 that at 60�c the line would have dropped so much that it is very unlikely that a reaction will take place after 60�c. So all in all my prediction was right. Biology Coursework ...read more.

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