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Investigation to Find the Optimum Rate of Anaerobic Respiration in Yeast.

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Introduction

Investigation to Find the Optimum Rate of Anaerobic Respiration in Yeast By James Taylor 10MA Plan I plan to find the optimum rate of anaerobic respiration in yeast by setting up an experiment where the yeast is heated in a glucose solution. When the yeast is heated it gives off gas so I will measure the amount of gas given off for my results. I plan to do my experiment safely by firstly wearing safety goggles. When using the apparatus I will use a heat proof and leave the Bunsen burner on the safety flame when I am not using it. I will make my experiment a fair test by keeping the following variables the same: the volume of water in the water bath, the amount of yeast and the volume of glucose solution used in the heated solution and the time the solution is left in the water bath. I predict that as the temperature of the solution is increased the collision rate of enzymes will increase with the substrate until it reaches its optimum temperature, which is when the denaturing will start to take place. ...read more.

Middle

The variable I will be changing is the temperature that the solution is heated to. I will be taking results at different temperatures these temperatures are 20�C, 30�C, 45�C, 60�C and 90�C. I will take each observation over 5 minutes recording how many bubbles are produced, and then I will find the average bubbles per minute. Analysis In my experiment I followed all the safety guidance's as I had laid out in my plan. From my results I can see that I have achieved enough results to give a reasonable answer the question. My Results Temperature (�C) Bubbles After 5 min 20�C 0 30�C 13 45�C 44 60�C 31 90�C 12 Averages Temperature (�C) Bubbles per Minute 20�C 0 30�C 2.6 45�C 8.8 60�C 6.2 90�C 2.4 I do think my results are reliable, as we had done preliminary practise experiments in which we could correct any mistakes we were making. If I could do the experiment again I would change the range of results slightly I would do a broad range at first, then followed by a narrower range nearer to where it looks like the optimum temperature would be. ...read more.

Conclusion

Evaluation Overall I think my experiment went really well but smaller changes could have been made which would make the results and the experiment more accurate. My results were quite good but probably not the best I could of achieved. The first set of the ones taken at 20�C could have been better, but as they were the first real ones we did something was bound to go wrong. Overall our results are pretty reliable as they stick to the right pattern. I don't think we got any very unusual results but , as I said the first set was slightly odd in that no bubbles were produced. I think this was because we had the delivery tube at a funny angle and so the gas was unable to get out. If I was to do the experiment again I would make several changes. These would be to set up the equipment very accurately to get the best results possible. Also to make both and wide and narrow range of results to find an accurate optimum temperature. I think those would be the only things major I would change as I was quite pleased with everything else. ...read more.

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