Investigation to Find the Optimum Rate of Anaerobic Respiration in Yeast.

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Investigation to Find the Optimum Rate of Anaerobic Respiration in Yeast

By James Taylor 10MA

Plan

I plan to find the optimum rate of anaerobic respiration in yeast by setting up an experiment where the yeast is heated in a glucose solution. When the yeast is heated it gives off gas so I will measure the amount of gas given off for my results.

I plan to do my experiment safely by firstly wearing safety goggles. When using the apparatus I will use a heat proof and leave the Bunsen burner on the safety flame when I am not using it.

        

I will make my experiment a fair test by keeping the following variables the same: the volume of water in the water bath, the amount of yeast and the volume of glucose solution used in the heated solution and the time the solution is left in the water bath.

I predict that as the temperature of the solution is increased the collision rate of enzymes will increase with the substrate until it reaches its optimum temperature, which is when the denaturing will start to take place. I think the optimum temperature will be 45°C, as most enzymes will stop working above the temperature. I found this out by doing preliminary research which states that ‘Enzymes are proteins that control chemical reactions in living things. Temperatures below 0°C and above 40°C destroy proteins and reduce enzymes activity’ (Source: Key Science Biology by David Applin pg. 174). So I have predicted that a temperature just above this will be its optimum rate before the enzymes begin denaturing

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The apparatus that I will be using in this experiment are:

  • Heat Proof Mat
  • Tripod
  • Bunsen Burner
  • Gauze
  • Beaker
  • Boiling Tube
  • Thermometer
  • Delivery Tube with Bong
  • Basin
  • Measuring Cylinder
  • Water
  • Yeast Solution
  • Glucose Solution

Diagram

When I do the experiment the variables I will not be changing are the volume of water in the water bath, the time the boiling tube is left in the water ...

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