Different sugar solutions will affect osmosis, as sugars such as lactose, maltose and sucrose will all work differently with osmosis as some are one cell thick and some are larger in size. This will affect osmosis because no sugars will be able to diffuse through the partially permeable membrane. If sugar solutions with a higher concentration then the mass of the chip will decrease because osmosis is working its way into the chip from the solution outside it. However, a sugar solution with low concentration osmosis will be working the opposite direction and the mass of the potato chip will increase. .
Preliminary Experiment
Below you will be able to see an experiment that I carried out previously which was investigating to see how the concentration of a sugar solution affects osmosis in a potato chip. This is the same as the one that I am going to carry out now, except the experiment that I am doing now covers more areas of the investigation.
Method: Once I had set up all the apparatus I needed to carry out the experiment, I got a potato and I punched a hole through the potato with a borer; I did this three times, so that I would obtain three potato chips. In addition, I cut each potato chip with a scalpel to a length of 3 cm each. When I did that I weighed each potato chip and I recorded the results in my results table (you will be able to see this below). After I did that I measured out sucrose solutions of different concentrations such as, 0.1,0.4,1.0 etc. each solution was measured accurately to 20cm3. When I did that I put the chips in to the three different sucrose solutions, and I waited for 15 minutes for the results. After I had waited 15 minutes I took the potato chips out and I blotted them using a paper towel. Once I did that I re – weighed them to see the mass difference from when I weighed them from the start of the experiment. You will be able to see a table with results below:
As you can see above this is my results table. In the first row I had used the 0.1m solution, in the second row I used the 0.4m solution and in the last row I used the 1.0m solution.
Conclusion: I thought that I had carried out the experiment successfully and appropriately. I discovered that in the less concentrated solutions such as 0.1m sucrose solution it was apparent that the concentration outside the chip is lower than the concentration inside the chip, so that’s why the mass of the chip increased. However, in the 1.0 m solution I discovered that the concentration outside the chip was higher than the concentration inside the chip so that’s why the mass decreased.
Equipment List
Below you will be able to see a list of equipment that I needed in order to carry out the experiment successfully and appropriately:
Potato
Scalpel
Boiling tubes
Beaker
Sucrose Solutions
Cutting Boards
Balance
Paper towel
Measuring Cylinder
Ruler
Borer
Method
Once I took out all of the apparatus and chemicals I needed to carry out the experiment I did the following:
- I used a borer to take out the potato chips from the potato. Altogether I had taken out 18 potato chips.
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Then, I measured out 20 cm3 of each solution such as, 0.1m, 0.2m, 0.4m, 0.6m, 0.8m and 1.0m. I measured out three solutions of each concentration, so that I ended up having 18 test tubes.
- I weighed my potato chips and I recorded the results in a table.
- After I did that, I put the potato chips in to the solutions at the same time and I started the stopwatch. I left the potato chips in the solutions for thirty minutes.
- After thirty minutes were up I took each potato chip out of the test tubes and I placed them on paper towels. Then I blotted them so that the solution was drained out of them.
- After I did that I re – weighed each potato chip and I recorded the results in to the table.
As you can see above this is how I carried out the experiment step-by-step.
Diagram
Safety
Below you will be able to see three safety tips that should be taken in to consideration when carrying out the experiment and using the apparatus:
Make sure when using the bora to take out potato chips from the potato, that your fingers are not in the way of the borer.
Make sure when you are cutting the potato chips to identical lengths, be careful to not place your fingers where the scalpel is going to cut the potato chip.
Make sure that when you are pouring the solutions in to the test tubes that you do not spill any of the solution on to yourself. This is harmless but may make your skin sticky.
Accuracy
I used six different concentrations so that I could investigate what would happen if I changed this variable. This also showed that as increased the concentration of the solution it became apparent that the mass would decrease and if I decreased the concentration then the opposite would happen. I did three repeats of each solution because this makes the investigation more accurate and this would make me more confident so that I would not have to repeat the experiment again to make sure that osmosis actually works appropriately. When I recorded my mass results I did them to two decimal places because it is more accurate and gives a clear indication where mass has increased and decreased. I did not do it to one decimal place because for example if I had a potato chip which weighed 1.65g before placed in the solution, and then the mass for the potato chip when taken out of the solution the mass was 1.69g. If I did not do it to two decimal places then those results would show no difference in mass. In addition I could have used a pipette to measure the solution as it is a good piece of equipment for measuring small amounts of solutions but it was more sufficient that I used a measuring cylinder for this experiment as it is specialized for measuring larger amounts of solution.
Prediction
I predict that with the less concentrated solutions such as 0.1, 0.2 and 0.4 molar, osmosis will occur in the direction to the potato chip as it has a higher concentrated solution. Therefore, this means that as the solution is less concentrated then the potato chip will allow in more water so the mass will increase. However, for higher concentrated solutions such as 0.6, 0.8 an 1.0 molar, osmosis will occur in the opposite direction and the mass of the potato chip will decrease as there is hardly any water in higher concentrated sugar solutions. I predict the trend of the graph should show a positive line to start with as less concentrated solutions make the potato chip increase in mass. Then it will start to decline and show minus/negative results as higher concentrated solutions make the potato chip decrease in mass. I predict the results will be able to be drawn on a line of best fit.
Fair test
Below I am going to show you the variables that are going to be kept the same:
The lengths of the potato chips are going to be a length of 3 cm, because this will affect the rate of osmosis if varied.
The volume of the solution is going to set at 20 cm3 because if the solutions are not the same then the mass of the potato chips will vary.
The duration of the potato chips left in the solution is going to all be kept in for thirty minutes because it takes a certain amount of time for osmosis to occur.
The identity of the solution is going to be kept the same i.e. Sugar solution. If the identity of the solution is changed then this will have different effects on the potato chip, also sucrose is a common solution and I know the effects of it from a previous experiment.
Below I am going to show you the only variable that is going to be changed:
The concentration of the solution is going to be changed i.e. 0.1, 0.2, 0.4, 0.6, 0.8, and 1.0m. This will be changed because I am trying to discover what happens to the rate of osmosis when the concentration of the solution is altered.
Analysis
Conclusion
After analysing my graph and table of results I discovered that the trend in results were what I expected. In addition, I was able to draw a line of best fit. However, the line only went through three results, but it was alright as the other results were just right next to the line. I thought that my results were correct as they started to show a positive increase in mass and then a gradual decline in mass as the concentration of the sucrose solution was increased.
After relating back to my prediction I discovered that my prediction was correct and showed a great relevance and clarity in my results. I knew my prediction was correct because as I looked at my results I did discover the drop and increase in change in mass, which proved correct, and also the graph trend also showed this.
My prediction matches my graph and results because firstly, in my graph it showed a positive trend at the start for example when the 0.1m was at 4.11% and the 0.2m was at 2.74%. In addition the mass started to decline onwards from 0.4m at –0.49% down to 1.0m at –7.78%. My results showed an increase in mass in the lower concentrated solutions because osmosis occurs the direction to the potato chip, which has a higher, concentrated solution. In addition, as the solution is less concentrated then the chip will gain mass. In addition, in the higher concentrated solutions osmosis will occur the opposite way, which will cause the chip to have a loss in mass. This is in the theory of the semi permeable membrane, which will only allow molecules, for example sugar molecules are too large to pass through the partially permeable membrane but on the other hand molecules such as water are small enough to diffuse through it. As the semi – permeable membrane only allows certain molecules through it also makes both solutions of even concentrations. So the higher concentrations will only have a little bit of water diffusing into the chip as there are a lot of sugar molecules, which cannot be passed through. For lower concentrations a lot of water will be diffused through the chip, as there is hardly any sugar molecules in it.
Evaluation
After looking back at the whole experiment and the results that I retrieved from it I think that I could make some more improvements to get accurate results. The improvements that I could make are as follows:
Firstly I could ensure that when I drop the potato chips in to the solutions I start the stopwatch exactly as I drop the potato chips. In addition, in the near future I could drop more potato chips.
Secondly, in the near future I could drop more potato chips in each concentration to retrieve more accurate results. This would also ensure the fact that osmosis is affected by various concentrations.
Lastly, I should keep the temperature constant i.e. sugar solution has to be set at a constant temperature. If I keep the temperature constant then I can retrieve efficient and precise results.
In order to make sure that osmosis works correctly I could try using different solutions in the experiment i.e. alkaline/acid solutions. If I use different solutions then this may produce different results. Also this will prove that osmosis is the diffusion of water molecules from where there is a high concentration to a lower concentration across a partially permeable membrane. When doing further test on osmosis I could change the identity of the vegetable/product. If I do this then this could make an affect to the rate of osmosis because some vegetables take longer for osmosis to occur i.e. potato chips took 30 minutes.
The evidence I retrieved from the experiment I thought was sufficient and fairly correct. The results from graph showed both an increases and decrease in mass, this thoroughly supported my prediction. There were not any anomalies on the graph. There were only three results, which were slightly off the line, which means that my accuracy wasn’t perfect, however I can correct this mistake by doing further tests and ensuring I do the procedures and cautions I stated above.
In conclusion, I think that I had carried out the experiment successfully and I hope to improve on my areas, which are of my weakest ability. I think that from not only doing further tests but ensure that I take measurements down correctly, and may be the range of the concentration could be increased and more potato chips, then I will obtain more accurate results. In addition, I think overall fairly sufficient good quality data was obtained.