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Investigation using thin layer of chromatography to separate photosynthesis pigment

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´╗┐Investigation using thin layer of chromatography to separate photosynthesis pigment Introduction Chromatography is a technique used for separating components or solutes of a mixture between two phases the mobile phase and the stationary phase. The stationary phase is a sheet of chromatography paper in paper chromatography. The mobile phase may either be a water-based liquid or a carbon-base organic solvent. For each chemical in the sample, there is a changing phase of balance between the stationary phase and the mobile phase. A paper chromatogram can be produced in a number of ways, for example if you have three green pens and you?re trying to identify which one was used to write a letter, samples of each ink are spotted onto a pencil line that?s drawn on a sheet of chromatography paper, some of the ink from the letter is then dissolved in a small amount of suitable solvent and is then spotted onto the same line. The paper is then inserted into a container with a deep layer of a suitable solvent in it and it?s important that the solvent level is below the line with the spots and the container is then covered to make sure the atmosphere in the beaker is saturated with the solvent vapour as this stops the solvent from evaporating. ...read more.


Mark the position of each colored pigment in pencil and take a photograph of the chromatogram as some colors fade rapidly 13. Measure the distance moved by the solvent front and the distance travelled by each pigment colors and record this in a table 14. Calculate the Rf value of each pigment and record the values in the table 15. Repeat the experiment using a paper chromatography 16. Finally using the information given, identify the pigments spots that?s been observed [c] H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0015.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0014.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0016.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0013.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0017.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0018.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0027.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0001.jpg Results and Calculations Paper Chromatography[d] Rf = distance travelled by sample Distance travelled by solvent Therefore, Rf of Paper chromatography = 4.1 Rf of component A = 1.0/4.1= 0.24 Rf of component B = 2.0/4.1= 0.49 Rf of component C = 4.0/4.1= 0.98 Component Distance travelled in cm Rf value Spot distance Solvent distance[e] A 1.0 4.1 0.24 B 2.0 4.1 0.49 C 4.0 4.1 0.98 TLC Chromatography[f] Rf = distance travelled by sample Distance travelled by solvent Therefore, Rf of TLC chromatography = 5.1 Rf of component A = 0.6/5.1= ...read more.


the experiment is the biggest way to improve our result as there was no time to repeat the experiment so if we had more time we would make sure to reduce most of our error[l], also making more concentrated extracted would improve our result as the extract wasn?t as concentrated as it should?ve been during the experiment. Risk Assessment * Propanone: Highly flammable and can cause eyes and skin damage. To reduce [m]the risk of this goggles should always be worn and ensure to keep away from naked skin. * Chromatography Solvents: Highly flammable and harmful, lid should be kept on the chromatography tank as much as possible also avoid breathing vapor and goggles should always be worn during the experiment. Reference https://www.britannica.com/science/chromatography http://science.jrank.org/pages/1464/Chromatography-Industrial-applications-chromatography.html https://www.chemguide.co.uk/analysis/chromatography/paper.html https://www.chemguide.co.uk/analysis/chromatography/thinlayer.html#top http://www.marz-kreations.com/Chemistry/Chromatography/Dyes/RF-Values.html http://science.jrank.org/pages/1464/Chromatography-Industrial-applications-chromatography.html [n] [a]Needs to be in italics [b]You need to write your method as if you did it using a past tense [c]Incorporate your pictures in your method [d]I need evidence of your work - picture [e]Swap the columns around [f]I need evidence of your work [g]Swap the columns around [h]This is a pigment!!!! [i]There was no hypothesis [j]capital [k]Why there are inaccurate measurements ? [l]Mention of making the extract more concentrated extracting more pigments. [m]Use of fume cupboard [n]Incorporate your pictures in your method ...read more.

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