• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month
Page
  1. 1
    1
  2. 2
    2
  3. 3
    3
  4. 4
    4
  5. 5
    5
  6. 6
    6
  7. 7
    7
  8. 8
    8
  9. 9
    9
  10. 10
    10

Investigation using thin layer of chromatography to separate photosynthesis pigment

Extracts from this document...

Introduction

´╗┐Investigation using thin layer of chromatography to separate photosynthesis pigment Introduction Chromatography is a technique used for separating components or solutes of a mixture between two phases the mobile phase and the stationary phase. The stationary phase is a sheet of chromatography paper in paper chromatography. The mobile phase may either be a water-based liquid or a carbon-base organic solvent. For each chemical in the sample, there is a changing phase of balance between the stationary phase and the mobile phase. A paper chromatogram can be produced in a number of ways, for example if you have three green pens and you?re trying to identify which one was used to write a letter, samples of each ink are spotted onto a pencil line that?s drawn on a sheet of chromatography paper, some of the ink from the letter is then dissolved in a small amount of suitable solvent and is then spotted onto the same line. The paper is then inserted into a container with a deep layer of a suitable solvent in it and it?s important that the solvent level is below the line with the spots and the container is then covered to make sure the atmosphere in the beaker is saturated with the solvent vapour as this stops the solvent from evaporating. ...read more.

Middle

Mark the position of each colored pigment in pencil and take a photograph of the chromatogram as some colors fade rapidly 13. Measure the distance moved by the solvent front and the distance travelled by each pigment colors and record this in a table 14. Calculate the Rf value of each pigment and record the values in the table 15. Repeat the experiment using a paper chromatography 16. Finally using the information given, identify the pigments spots that?s been observed [c] H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0015.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0014.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0016.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0013.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0017.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0018.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0027.jpg H:\Documents\assigment C pics\Assigmnet c pictures\IMG-20181216-WA0001.jpg Results and Calculations Paper Chromatography[d] Rf = distance travelled by sample Distance travelled by solvent Therefore, Rf of Paper chromatography = 4.1 Rf of component A = 1.0/4.1= 0.24 Rf of component B = 2.0/4.1= 0.49 Rf of component C = 4.0/4.1= 0.98 Component Distance travelled in cm Rf value Spot distance Solvent distance[e] A 1.0 4.1 0.24 B 2.0 4.1 0.49 C 4.0 4.1 0.98 TLC Chromatography[f] Rf = distance travelled by sample Distance travelled by solvent Therefore, Rf of TLC chromatography = 5.1 Rf of component A = 0.6/5.1= ...read more.

Conclusion

the experiment is the biggest way to improve our result as there was no time to repeat the experiment so if we had more time we would make sure to reduce most of our error[l], also making more concentrated extracted would improve our result as the extract wasn?t as concentrated as it should?ve been during the experiment. Risk Assessment * Propanone: Highly flammable and can cause eyes and skin damage. To reduce [m]the risk of this goggles should always be worn and ensure to keep away from naked skin. * Chromatography Solvents: Highly flammable and harmful, lid should be kept on the chromatography tank as much as possible also avoid breathing vapor and goggles should always be worn during the experiment. Reference https://www.britannica.com/science/chromatography http://science.jrank.org/pages/1464/Chromatography-Industrial-applications-chromatography.html https://www.chemguide.co.uk/analysis/chromatography/paper.html https://www.chemguide.co.uk/analysis/chromatography/thinlayer.html#top http://www.marz-kreations.com/Chemistry/Chromatography/Dyes/RF-Values.html http://science.jrank.org/pages/1464/Chromatography-Industrial-applications-chromatography.html [n] [a]Needs to be in italics [b]You need to write your method as if you did it using a past tense [c]Incorporate your pictures in your method [d]I need evidence of your work - picture [e]Swap the columns around [f]I need evidence of your work [g]Swap the columns around [h]This is a pigment!!!! [i]There was no hypothesis [j]capital [k]Why there are inaccurate measurements ? [l]Mention of making the extract more concentrated extracting more pigments. [m]Use of fume cupboard [n]Incorporate your pictures in your method ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Marked by a teacher

    AN INVESTIGATION OF THE DIFFERENT SPECIES OF MAYFLY WITHIN THE POOL & RIFFLE

    4 star(s)

    I then needed to put the net in of the trays of water to remove the invertebrates in the nets. I had to use a spoon to find and lift the mayflies in the midst of the invertebrates from the tray to identify them using the identification key, before I put them in the other tray.

  2. Tank Chromatography.

    The repeat was made due to the width of the paper and therefore gave us a repetition of results, enabling us to make averages of the two to give more reliable end results. Once the initial ink spots were placed, these were allowed to dry for 10 seconds and then

  1. Separation of photosynthetic pigments by paper chromatography.

    ether into the boiling tube to a depth of no more than 15 mm. Seal the tube with a stopper for about 10 minutes so that the inside of the boiling becomes saturated with vapour; * Suspend the strip of paper in the boiling tube.

  2. How temperature affects the rate of photosynthesis.

    made when the light intensity changed and might have had an affect on the rate of photosynthesis.) 12. Allow the Elodea to acclimatise to for 2 minutes. (Allowing acclimatising time to the elodea would ensure that the elodea gets accustomed to the new light source and the new surrounding temperature.)

  1. An Investigation into Species Diversity with distance along a Pingo.

    The rabbit population also fell due to myxomatosis in the 1950s. The decline in grazing has encouraged natural succession converting the grassland into scrub and woodland. Recently the rabbit population has begun to recover and cattle have also been introduced onto the Common to maintain the rare grassland habitat.

  2. Absorption Spectrum of Chlorophyll.

    The normalized spectra are shown in Figure 7 with error bars omitted for visual clarity. Figure 7. Comparison of Spinach Extract Spectra using Normalization Figure 7 shows that the absorption spectra from the four different extracts of spinach agree very closely.

  1. "Separating colors in leaves using Chromatography"

    filters * Shallow pan * Luke warm tap water * Masking tape * Pen * Morter and pestle (if you don't have one use the end of a hammer, or anything that you can use to crush will be fine)

  2. How Temperature Effects the Movement of Pigment Through Cell Membranes

    However at high temperatures a process pertaining to a change in the structure of a protein from regular to irregular arrangement of the polypeptide chains, causes a physical disfigurement called denaturing. Once a protein is denatured it can no longer be used by the body as it lacks its necessary

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work