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Measure the amount of oxygen given off when we react catalase (enzyme) with hydrogen peroxide (substrate).

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Introduction

Aim My aim for this investigation is to measure the amount of oxygen given off when we react catalase (enzyme) with hydrogen peroxide (substrate), this means that I aim to investigate the effect of hydrogen on the activity of catalase. Background Information Enzymes such as Catalase are protein molecules that are found in living cells. They are used to speed up specific reaction in the cell. The enzymes are all very specific as each enzyme performs one particular reaction. Catalase is an enzyme found in many foods such as potato and liver. It's used for removing Hydrogen Peroxide from cells. Hydrogen Peroxide is the poisonous product of metabolism. Catalase speeds up the decomposition of Hydrogen Peroxide into oxygen and water. Variables I will make sure I keep the following variables the same. Size of potato: The Size of a potato cube would effect the investigation because the size of the cube would effect the number of cells in contact with the hydrogen peroxide. Temperature: Temperature can increase and decrease the rate of reaction. Concentration: The concentration of the hydrogen peroxide would effect the rat of reaction. Dimensions of potato: If the potato has more surface area showing that will effect the number of cells in contact with the hydrogen peroxide. ...read more.

Middle

After the air bubbles have come out I will quickly place the burette over the delivery tube and I will start the stop clock. Then when the water level has moved down 2cm cubed, I will stop the clock. Then I will record my results into a table and then I shall repeat steps 6 to 10 with the following regime: Hydrogen Peroxide Water 5mls 0mls 4mls 1ml 3mls 2mls 2mls 3mls 1ml 4mls Results Hydrogen Peroxide (mls) Time 1 (secs) Time 2 (secs) Time 3 (secs) Average Time (secs) 5mls 74 secs 90 secs 92 secs 85 secs 4mls 110 secs 95 secs 110 secs 105 secs 3mls 118 secs 117 secs 140 secs 125 secs 2mls 157 secs 150 secs 158 secs 155 secs 1mls 167 secs 175 secs 170 secs 171 secs Graph The graph of my results is on the next page. Interpretation From the results I have collected, I have now plotted a graph, showing the decomposition of the H2O2. If we look at this graph we can clearly see that points plotted on the graph are not that far away from the line of best fit, showing a quite strong relationship but a negative one. ...read more.

Conclusion

This would have given me more results and when I would of came to plotting my graph there would have been more points producing a greater accuracy and a better line of best fit with which I would of been able to get stronger conclusions from. To make the experiment as accurate as possible, I repeated it three times and used the averages of all the results to plot a graph with a line of best fit. I did my best to keep all the variables apart from the one I was testing (Hydrogen Peroxide) the same. However in practice it is impossible to do using the basic apparatus. For example: It is impossible to precisely measure out the exact amounts of Hydrogen Peroxide and Distilled Water each time, as the scale on the measuring cylinder only shows the measurement to the nearest 1mm cubed. I think my timing was as accurate and reliable as it could have been, but only a forth or fifth experiment would back that up fully. The equipment we used was reasonable but not the best, but considering the conditions we were based in, it was the best we could of got, I also think contamination was minor so that would have not caused a problem. ...read more.

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