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Measuring enzyme Rates of Reaction

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Introduction

Measuring enzyme Rates of Reaction Aim The aim of this experiment was to use four different ways of measuring the rate of a chemical reaction by conducting a series of experiments finding out how quickly enzymes work. Method - {SEE SHEET} Results Summary Enzyme End Point Min (s) End Point Min Rate Of Reaction Amylase (Starch) 1:30 1.5 0.66 Trypsin (Marvel) 2:05 2:08 0.48 Trypsin (Photo film) 9:48 9.8 0.15 Catalase Hydrogen Peroxide & Yeast 15 0.25 4 Interpretation of results From my results I see that the fastest method was method 4 the floating disc method. Using this method for a future experiment could turn out to be difficult because the reaction occurs quickly. But this method could be repeated. Using the disappearance of a substance method could be accurate way of measuring the rate of reaction but would be difficult one to do as the end point should be fully understood before the reaction. ...read more.

Middle

3 The photographic film contains a protein. The Trypsin mixes with the protein and breaks it down the photographic film becomes white/colourless - this is the end point. 4 The yeast contains the enzyme Catalase. This Catalase breaks down the Hydrogen Peroxide (2H2O2) and releases oxygen bubbles that move the filter paper (with yeast) upwards. The time is then recorded. Interpreting Class Results There are many differences in the results. The ranges on most (one exception maybe method 2) experiments are very high. Some of the reasons for this are discussed in the Sources of Error section. Just from these values I cannot determine whether the larger range is correct or the lower range is correct. However, by looking at the mode, for example on experiment 2, 0.25 comes up 3 times this could mean the real figure lies around at area. ...read more.

Conclusion

* Deciding the end point; it is difficult to know when the photographic film is clear (method 3), or the exact moment when the I/KI colour stays the same (method 1). In the case above I could have used a colorimeter for more accurate measurements or kept a reference colour to know when Marvel disappears (method 2). The benefits of this experiment are that it is quick and relatively simple to do. One deterrent from this experiment is its accuracy or finding the end point. This could be overcome by repeating measurements (knowing when the end point is), so the results are valid and consistent. Conclusion The four methods I did are very simple ways of measuring the rate of reaction in terms of enzyme activity. To obtain accurate measurements the tests need to be repeated 3-4 times. And it is vitally important to know when the end point of the reaction is. 1 ...read more.

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