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Micro-organisms,most effective antibiotic to act against the two types of bacterium: E.coli and B.subtilis.

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Introduction

Micro-organisms: Use of antibiotics In this coursework I will be working with two different types of bacteria, Escherichia coli 0157 and Bacillus subtilis. They are usually shortened to E.coli and B.subtilis. I am going to see which antibiotic is most effective antibiotic to act against the two types of bacterium: E.coli and B.subtilis. Risk Assessment Substance/Apparatus Hazard Risk Precaution Emergency Action Glassware Dangerous if glass is broken. Low risk and perfectly safe if used properly. Do not try to pick up pieces of broken glass with your hands, especially from a wet sink. Report all breakages immediately so that staff can deal with it. Inform all students of any broken glass so they move away from it and avoid injury. Inform staff so they can deal with it. Seek medical attention. Trip Hazards. Bags, coats, chairs Low risk if bags, coats and chairs are put or stored in a safe area. Put baggage etc well under benches, away from the working area or store at the front of lab. Inform staff and seek medical attention. Help the person if possible. Bunsen Burner Could burn the skin and can set clothing on fire Low risk if used correctly and safely. ...read more.

Middle

The method I used included aseptic technique. Aseptic technique is a set of specific practices and procedures performed under carefully controlled conditions with the goal of minimizing contamination by pathogens. Aseptic technique helps to prevent or minimize infection Before I begun the practical, I sprayed the area in front of me with a disinfectant. Stage 1 1. I first put on a laboratory coat to protect me and my clothes and wore safety glasses to protect my eyes and also disposable gloves since I will be dealing with bacteria. 2. I placed the Bunsen burner on the mat and lit it to leave it on invisible flame. 3. I then waited till the molten agar is hand hot so I could hold it comfortably in my hand and then unscrewed the bottle gently and carefully. 4. To kill of any germs at the top of the bottle and to sop decontamination I passed the neck of the bottle through the flame a few times. 5. I gently unscrewed the bottle of bacteria - E.coli and passed the neck of the bottle through the flame. 6. I then added the E.coli to the bottle of agar, passed the bottle gently through the flame then screw the lid back on. ...read more.

Conclusion

Diagram of what I did: I tested the antibiotics on a bacterial lawn. A bacterial lawn is a continuous cover of bacteria on the surface of a growth medium. It is the appearance of bacterial colonies when all the individual colonies on a Petri-dish agar plate merge together to form a field of bacteria Table to show which antibiotic was the most effective E.coli Bacillus subtilis Antibiotic Diameter of clear circle in centimetres(cm) Most effective by rank (1 being the most effective) Diameter of clear circle in centimetres(cm) Most effective by rank (1 being the most effective) Ampicillin 1.4 2 - - Erythromycin - - 2.7 2 Penicillin - - - - Sulphafurazole - - - - Chloramphenicol 1.6 1 2.8 1 Methicllin - - 0.7 5 Streptomycin 1.3 3 2.2 3 Tetracycline 1.3 3 1.7 4 The diameter of the clear zone around each antibiotic indicates how effective it is at killing the bacteria To decide which the most effective antibiotic is I will look at the 'Most effective by rank' column to see which antibiotic is ranked 1st. I ranked each antibiotic by measuring the diameter of the clear zone around each antibiotic. The larger in length the diameter, the more effective it is. The most effective antibiotic is therefore 'Chloramphenicol' since it had the largest diameter for both types of bacteria. ...read more.

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