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Movement of Pigment through Cell Membranes

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Investigation Movement of Pigment through Cell Membranes Aim The aim of the investigation is to identify the effect of temperature of the movement of pigment through cell membrane of beetroot cells. Prediction and Reasoning I predict that the temperature will effect the movement of the beetroot pigment through the cell membrane. I believe an increase in temperature would result in an increase in the amount of pigment being released out of the cell. I base my prediction on the fact that an increase in temperature would provide the pigment molecules with more kinetic energy and therefore the rate of diffusion will increase. The increase in temperature would also denature (damage or change the characteristics of) the cell membrane resulting in more pigment being lost. Allowing the pigment to diffuse more rapidly throughout the two dimensional surface of the membrane. In the diagram the pigment from the beetroot (section A) would pass more readily into the water (section B) if it had more energy and also would pass quicker if the membrane becomes denatured by the increased kinetic energy of the molecules and the high temperatures. ...read more.


Leave each beetroot cylinder in its beaker for exactly thirty minutes. Once the thirty minutes are over shake the test tube and remove the cylinder. Do this for all ten test tubes and you should end up with ten test tubes containing water stained pigment from the beetroot. Finally place each solution into its own corvette and place it into to the colorimeter and record the readings. Diagram Variables The variables included in this experiment are as follows: -The temperature of the beaker. -The amount of beetroot added. -The duration of time the test tubes are in the warm beaker. The variable which we chose to look at in more depth was the temperature of the beaker which would heat surrounding water in the test tube to where there would be a net movement of pigment. From this we can determine what the best temperature is for diffusion to take place. Fair Test It is vital that a fair test is carried out otherwise the results would be misleading and lead to a false conclusion and evaluation. To help make this investigation a fair test each substance and solution was measured accurately and to the nearest possibilities. ...read more.


have been much more reliable and much more accurate as an average result could have been found which would have reduced the amount of anomalies appearing. A few anomalies did appear in the results this could have been down to the colorimeter and the amount of water there was in the cuvette before placing it in the colorimeter as this varied. Suitability of evidence and improvements As we used a range of ten different temperatures the results would have been more reliable as if a anomaly appeared it would be clearly spotted within the results. Changes or improvements that could be made if I was to carry out the experiment again are that I would make sure that each beetroot cylinder would have been the exact same length, weight and thickness. Also I would make sure I had a much better way of measuring the amount of light absorption as the colorimeter was unable to measure some of the values correctly. Further Work For further work I would use different substances which would allow me to compare beetroot to another substance and therefore allow me to see hoe different substances actively different substances allow the movement of pigment through cell membranes. ...read more.

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