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Observe how much oxygen is given off, when we change the concentration of catalase in potato discs, during the reaction with hydrogen peroxide.

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Introduction

Catalase Investigation In this investigation we wanted to observe how much oxygen is given off, when we change the concentration of catalase in potato discs, during the reaction with hydrogen peroxide. Related knowledge about catalase * The temperature and pH affect catalase activity * It works best at the pH 7 - and room temperature * Catalase becomes denatured if subjected to high temperature or pH other than 7 * Affects active site shape activity Apparatus * Scalpel * Ruler * White tile * Tweezers * Test tube with side arm and tubing * Delivery tube * Retort stand * Bung * Water bath * Potato Cylinders * 20% Hydrogen peroxide * 2 measuring cylinders * Stop clock * ...read more.

Middle

Method 1) Set up apparatus (shown in diagram) 2) Cut Potato chip in to 1mm discs with scalpel 3) Measure out 10ml of hydrogen peroxide and add to a test tube with side arm 4) Quickly, we then added 2 potato discs to the hydrogen peroxide. This tube was then connected to a measuring cylinder in a bowl of water; this measured how much oxygen was being given off 5) We timed the experiment for 5 minutes and checked the measuring cylinder to see how much oxygen had been given off. 6) We added this information to a table Table Number of Discs Run 1: Volume of Oxygen given off Run 2 Average Volume of Oxygen given off 2 2mls ...read more.

Conclusion

* Do not eat or drink during the experiment, food or drink could have been next to a leaking bottle of hydrogen peroxide, which could get on food or drink. Conclusion My results prove that my prediction was right; that the more potato discs you add the bigger the results will be. My results show that as you add more pieces of potato, the amount of oxygen also increases. The graph shows that the more pieces of potato you add to the test tube, the higher the amount of oxygen. I have found out that the more surface area you have, the more enzyme is released; this causes the hydrogen peroxide to produce more oxygen quicker as more enzyme is added. ...read more.

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