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Osmosis in potatoes at different concentrations of sucrose solutions.

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Introduction

Biology investigation - Osmosis in potatoes at different concentrations of sucrose solutions. Planning Aim: The aim of this experiment is to find out how solutions of different strength affect the osmotic movement. Preliminary work: For the preliminary work, I did a try out test to see if the method I had works. Also, I wanted to use the results I have after completing the experiment so that I can have an overview of how sucrose solutions of different strengths affect the diffusion of water in potato chips. I would also use what I have concluded at the end of the experiment to make a final decision of whether to use the method would produce the best and most reliable results. I thought that the lengths of the potatoes will change after the experiment because there had to be some movement in or out of the chip. So I thought that the size of the potato would increase or decrease depending on the solution it is in. In the method of the try-out test, I had came up with is to measure the lengths of the potato chips before and after the experiment to see if there are any changes to the size of the potato chip due to the movement caused by osmotic water diffusion. The method I used for the try-out test: 1. I made 6 potato chips by using a potato borer from the one whole potato and made sure that they have the same length. 2. Using a measuring cylinder, I measured the amounts of each solutes separately according to the ratio of the I made sucrose concentrations of 6 various concentrations ranging from distilled water to 1 Molar sucrose concentration (and poured the specific amount when mixing a certain solution and pour them into separate boiling tubes) using a specific sucrose: distilled water ratio and labeled them with their sucrose concentration separately. ...read more.

Middle

So therefore, the cell membrane must stop its permeability. Variables: In this experiment, the things that could affect the result are: 1. The concentration of the sucrose solution 2. The size of the potato chips (diameter and length of the chip) 3. The quantity of the sucrose solution 4. The quantity of the potato chip 5. The amount of time the chip has been in the solution Experimental variable: In order to provide a fair test, I have decided to use different concentrations of sucrose solution as the experimental variable. Fair test: 1. To ensure that the experiment is a fair test, I would make sure that the potato chips all have the same volume (diameter, length and similar masses). To do that, I could use the same potato borer, knife and ruler to make the six potato chips. 2. To make sure that all the potatoes have the same rate of osmotic movement, I would make all the potato chips for each set of results from one single potato so that there would be an accurate set of result. 3. To make sure that the sucrose concentration to distilled water are the same, I first measured the amounts of sucrose solution and distilled water needed for the solution needed before mixing them together. 4. Apart from those, I think I will need to make sure that the amount of sucrose solution in each of the test tubes are the same before I put the potato chips in the solution. 5. Since I could place all the potato chips into the solutions and start timing all at the same time, I need to put time them separately. Method: 1. I make the potato chips with a potato borer and measured their lengths, their mass and record the information. 2. Make the solutions, (Note: make sure that they are all at the right strength) pour them in a test tube, leave it in a test tube rack and label each of them. ...read more.

Conclusion

Otherwise, each mass change would be following a difference trend pattern and so therefore it will be impossible to actually draw a line of best fit. Also, the difference in the lengths of the potato chips had nearly no change. This also shows that my method and experiemt was planned out quite fairly because otherwise the lengths changes would not be little like only increasing o.5 mm or 1mm in the lengths in my experiment. If I was to do this experiment again, what I would change in the methodology is to be more careful when measuring the readings. This is because when I did the experiments, I thought that I was not going to finish the expeiment on time. So at the beginning of the experiment when I was measuring the amount of sucrose solution and distilled water, making the potato chips and making sure that they're the same length, I rushed through them. I think that's probably why I got an unexpected result for the potato chip in the 0.8M solution. Even though there was not a mistake for every single other little experiment, but there might have been some slight mistakes. Evaluation of Results: I think that the results I got was quite reliable and accurate despit there was something that has gone wrong with the potato chip in the 0.8M solution. From the graph of my results, I could see that it was an anonymous result. Overall, I think that the result are quite accurate because even though there was only one result that is out of the pattern from the rest. This does not matter very much because despite there may be a certain mistake for the osmotic movement in the 0.8M solution, but the other results are all very similar. The similiarities between them would still provide evidence for the theory of osmosis to be true even without the result for the 0.8M solution. Also, we could still predict the result for it using the line of best fit in the graph. ...read more.

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