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PLANNING:The solution was left undisturbed for 24 hours. This gave the alcohol enough time to act on the egg. After 24 hours the length of coagulant, was first measured with a ruler.

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Introduction

PRELIMINARY EXPERIMENT. REQUIREMENTS: SOLUTIONS USED. 1) Alcohol solutions: ~ 30% ~70% 2) Hydrochloric acid: ~0.005M ~0.02M ~0.08M Apparatus used: 1) Colorimeter 2) Beakers. 3) Graduated Cylinders. 4) Pipettes. 5) Droppers. 6) Test tubes. 7) Rubber stoppers. 8) Reagent bottles. 9) Test tube stand. 10) Glass rod. MATERIALS USED. Egg white. PREPARATION OF SOLUTIONS: 1) Alcohol: The concentration of the stock alcohol was 100% and hence needed to be diluted to the required concentrations that had to be used during the experiment. To prepare a 100 ml solution of 30% alcohol: First I measured 70 ml of water using a measuring cylinder then I measured 30 ml of alcohol in another measuring cylinder. Then I poured the two solutions into a reagent bottle and stirred it with a glass rod. To prepare a 70% solution of alcohol: First I measured 30 ml of water in a measuring cylinder (care being taken to wash the measuring cylinder before use).Then I measured 70 ml of alcohol in a measuring cylinder. Then I poured the two solutions into a reagent bottle and stirred it with a glass rod. ...read more.

Middle

Then I measured 1 ml of the acid using a measuring cylinder. This also I poured into the reagent bottle. Then I stirred the two using a glass rod. For the preparation of the acidic solution of concentration 0.08M: n= cV =0.08*50/1000 =0.004 moles n= cV =0.004*1000/1 =4 ml For 50 ml of 0.08M of acid I added= 4ml of acid and 46 ml of water. I measured 46 ml of water using a measuring cylinder and poured it into a reagent bottle. Then I measured 4 ml of the acid using a measuring cylinder. This also I poured into the reagent bottle. Then I stirred the two using a glass rod. EXPERIMENT. The procedure used was the same for all solutions the only difference was that there were different solutions of different concentrations being used. For example the procedure used for 30% alcohol was: First I measured 5 ml of egg white (albumen) using a measuring cylinder. I poured the egg white into a test tube. Then I measured 10 ml of the test solution which is the 30% alcohol solution using a measuring cylinder. ...read more.

Conclusion

The second equally important way to protect myself from laboratory hazards was to be knowledgeable about the materials with which I was working and respectful of the danger they may present. Safety precautions: 1) The experiment involves handling glassware, which was breakable. I made sure to handle all glass with proper care, and making sure not to drop or knock over any pieces. 2) The experiment involved handling acid and base solutions, which can splash if, dropped. I made sure to handle all solutions with proper care, making sure to protect myself from spills. 3) I made sure to wash all the apparatus thoroughly before and after use to prevent contamination when the apparatus are used again. RESULT TABLE: 30% alcohol solution TRIALS %TRANSMISSION AVERAGE 1 18% 18.5 2 19% 2-Concentration-70%: TRIALS %TRANSMISSION AVERAGE 1 2 1.75 2 1.5 CONCENTRATION OF THE ALCOHOL. THE LENGTH OF THE COAGULANT. 30% 2.3 cm 70% 6.9 cm ACIDS: ACID CONCENTRATION TRIAL % TRANSMISSION AVERAGE 0.005M 1 66 67 3 68 0.02M 1 31.5 32 2 32.5 0.08M 2 20 20.5 3 21 CONCENTRATION OF ACID. THE LENGTH OF COAGULANT. 0.005 M 0.9 cm 0.02 M 1.6 cm 0.08 M 3.7 cm ...read more.

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