PLANNING:The solution was left undisturbed for 24 hours. This gave the alcohol enough time to act on the egg. After 24 hours the length of coagulant, was first measured with a ruler.

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PRELIMINARY EXPERIMENT.

REQUIREMENTS:

SOLUTIONS USED.

1) Alcohol solutions:

~ 30%

~70%

2) Hydrochloric acid:

~0.005M

~0.02M

~0.08M

Apparatus used:

1) Colorimeter

2) .

3) .

4) .

5) Droppers.

6) Test tubes.

7) Rubber stoppers.

8) Reagent bottles.

9) Test tube stand.

10) Glass rod.

MATERIALS USED.

Egg white.

PREPARATION OF SOLUTIONS:

1) Alcohol:

The concentration of the stock alcohol was 100% and hence needed to be diluted to the required concentrations that had to be used during the experiment.

To prepare a 100 ml solution of 30% alcohol:

First I measured 70 ml of water using a measuring cylinder then I measured 30 ml of alcohol in another measuring cylinder. Then I poured the two solutions into a reagent bottle and stirred it with a glass rod.

To prepare a 70% solution of alcohol:

First I measured 30 ml of water in a measuring cylinder (care being taken to wash the measuring cylinder before use).Then I measured 70 ml of alcohol in a measuring cylinder. Then I poured the two solutions into a reagent bottle and stirred it with a glass rod.

2) Acid:

The concentration of the stock solution of hydrochloric acid in the laboratory was 1M solution this needed to be diluted to different concentrations that had to be used for the experiment.

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To dilute the acidic solution I used the equation:

         

For the preparation of the acidic solution of concentration 0.005M.

n= cV

n= 0.005*50/1000

 = 0.00025 moles

n= cV

where n= no. of moles

          c= concentration of stock solution

          V= volume

n= cV                                              V= x + y                                

0.00025=1*V/1000        x= volume of acid

V=0.00025*1000/1        y= volume of water

 =0.25ml

        

For 50 ml of 0.005M acid I added = 49.75 ml of water +0.25 ml ...

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