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Rate of reaction between the enzyme CatalaseAnd Hydrogen Peroxide

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Introduction

Biology coursework SC1 Rate of reaction between the enzyme Catalase And Hydrogen Peroxide Background Enzymes are biological catalysts that speed up reactions. Catalase is an enzyme, and one of the fastest ones that are known to man. It breaks down Hydrogen Peroxide at 30 centigrade at a rate of 100,000 molecules per second. This is an extremely fast rate of reaction. Catalase is found in organisms such as potatoes. Therefore, as a source of Catalase, I will use a potato in order to obtain it. Enzymes are catalysts, and so, once they are used up, they can be re-used over and over again (in theory). Hydrogen Peroxide Catalase enzyme oxygen + water [Substrate] [Enzyme] [products] 2H2O2 Catalase enzyme O2 + 2H2O PLAN I will be testing the surface area of the potato slices. The more slices that I cut out, the greater the surface area will be. I will obtain the potato slices by using a potato borer, in which I will take out a whole chunk of potato, all of which will be of the same diameter, and then in order to obtain potato slices of equal surface area, I will simply cut off little plates that are 1 cm long to ensure they are of the same size, and so the same surface area of the potatoes is exposed to the solution, and so more enzymes can react with the hydrogen peroxide in the solution, because the reaction takes place on the surface of the potato, as this is where the reactions take place because the enzymes are on the surface of the potato. ...read more.

Middle

have to make time count, and so I have decided not to go on further than for 5 minutes (i.e 300 seconds) otherwise, it would be a waste of time. Therefore, I have decided to go for the combination of 0.5M concentration because, at a minimum (with only 1 potato slice), in the preliminaries, It has come out as 289 seconds, which is just below the 5 minute mark. Therefore, it should be easier to record the results when the time comes to record those long experiments, but should not in turn be too quick when it comes to recording the time when I have to try and measure the experiment when it is at maximum, which will be at 0.5M with 5 potato slices. I have found out that the measuring cylinders do not all start from 0 when you have turned them upside down, and so then there is a little space before I can start reading. However, I have also found that when I plug the bung onto the top of the reaction tube, there has to be some loss for the compressed air, and this is squeezed out through the side tubing, and into the measuring cylinder. Therefore, the few mm of space left in the measuring cylinder when turned upside down is cancelled out. However, 1 problem which I have found to be significant is that of which I cannot place the bung on quick enough. ...read more.

Conclusion

Therefore, the trend seemed to be that of the potatoes not quite keeping up to my hypothesis of that of staying in harmony of proportionality. In order to make sure these results were OK, I would do more repeats next time round. I think an ideal extension to this experiment would be to redo the experiment, but using the centrifuge, and then to compare results, to see if the results would have come out much different. Also, you could redo this experiment with different enzymes, such as lipase, which is also found inside the human anatomy. Then again, you could have tried out other forms of the enzyme, or potato, such as mashed potato form, or cubic form. Or, I could vary something else, not Surface area, such as temperature, or concentration of the hydrogen peroxide. Even, you could try and make the experiment as a whole work at a different temperature as a constant, e.g. 30 degrees centigrade, and then go and vary something like the concentration, and then compare back. As for improvements to this experiment, I could go and try to get more results. Also, I could try more accurate equipment, and I would make all things much more accurate, by not depending completely on the eye (such as on the tilting measuring cylinder), but instead by more use of accurate equipment such as scales. Also, I could go and try to become more useful with the equipment myself - I personally am prone to mistakes. Therefore, the ideal experiment would be performed by hi-tech robots (sadly, they don't have these in schools nowadays). Shinyoung Kim 5 Aleph ...read more.

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