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Rate of reaction of Trypsin.

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Rate of Reaction of Trypsin - An Investigation - Planning Aim: To investigate how the concentration of trypsin affects the rate of reaction. Background Knowledge: Enzymes exist in all living things. They are composed of polymers of amino acids and are produced in living cells. Each cell contains several hundred enzymes, which catalyse a vast number of chemical reactions. Enzymes are known as biological catalysts as they dramatically increase the rate at which reactions occur within living organisms, without being 'used up' or effecting the reaction in any other way. Trypsin is an enzyme which digests proteins, and in this investigation I will use it at different concentrations to see how it affects the rate of reaction. Reaction: This is the reaction that we will be investigating: Changing milk protein (- white, large, and insoluble molecules) Amino acids (- colourless, small, and soluble molecules) Hypothesis: Reaction rates are explained perfectly by the Collision Theory. It says that the rate of a reaction depends on how often and how hard the reacting particles collide with each other. The basic idea is that particles have to collide in order to react, and they have to collide hard enough as well. ...read more.


Precautions: As a precaution, I have limited my contact with the test tubes, as my body heat will raise the temperature, increasing the rate of reaction. I will also monitor the temperature using a thermometer so that I can ensure that it stays constant and does not disrupt the results of the experiment. I have also, when filling a measuring cylinder with trypsin, placed it first on a flat surface so that its measurement can be taken accurately. Also, I have read the measurement from underneath the meniscus and not the top. To ensure that quantities of the various chemicals are measured accurately, I have used the smallest pipettes or measuring cylinders for each quantity. For example, to measure 5ml of trypsin, I used a 5ml pipette. In this way, more accurate measurements can be made since more detailed gradations are available on the apparatus. Safety precautions that have been taken are the wearing of goggles throughout the experiment to avoid chemicals getting into the eye. Fair Test: To keep this investigation a fair test I will make sure that I will always measure out exactly 5ml of each substance before I mix them. ...read more.


For example, firstly, I could test the effect of concentration on the rate of reaction on other enzymes too, whose results could be used to back or test the conclusion found in this investigation. One example of this kind of experiment would be the reaction between hydrogen peroxide and liquidated celery. It could also have been hydrogen peroxide on a liver, but this reaction much too quickly to be able to record the results with any accuracy. This is because the liver has too many catalase enzymes, so the reactions are made a lot quicker making the investigation unfair. The experiment will be conducted as follows: Apparatus: * water basin * conical flask * bung, * delivery tube * measuring cylinder * syringe * spatula * stopwatch * electric scales (for increased accuracy) I will be timing the amount of oxygen that passes through the delivery time in a certain amount of time, after mixing the celery and Hydrogen Peroxide. I will take measurements from the side of the measuring cylinder every 30 seconds and note them down. From my results I would have found that the higher the concentration of hydrogen peroxide, the quicker the reaction rates producing oxygen. Suraj P. Nakum 10I GCSE Science Coursework Page 1/6 ...read more.

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