• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Rate of reaction of Trypsin.

Extracts from this document...

Introduction

Rate of Reaction of Trypsin - An Investigation - Planning Aim: To investigate how the concentration of trypsin affects the rate of reaction. Background Knowledge: Enzymes exist in all living things. They are composed of polymers of amino acids and are produced in living cells. Each cell contains several hundred enzymes, which catalyse a vast number of chemical reactions. Enzymes are known as biological catalysts as they dramatically increase the rate at which reactions occur within living organisms, without being 'used up' or effecting the reaction in any other way. Trypsin is an enzyme which digests proteins, and in this investigation I will use it at different concentrations to see how it affects the rate of reaction. Reaction: This is the reaction that we will be investigating: Changing milk protein (- white, large, and insoluble molecules) Amino acids (- colourless, small, and soluble molecules) Hypothesis: Reaction rates are explained perfectly by the Collision Theory. It says that the rate of a reaction depends on how often and how hard the reacting particles collide with each other. The basic idea is that particles have to collide in order to react, and they have to collide hard enough as well. ...read more.

Middle

Precautions: As a precaution, I have limited my contact with the test tubes, as my body heat will raise the temperature, increasing the rate of reaction. I will also monitor the temperature using a thermometer so that I can ensure that it stays constant and does not disrupt the results of the experiment. I have also, when filling a measuring cylinder with trypsin, placed it first on a flat surface so that its measurement can be taken accurately. Also, I have read the measurement from underneath the meniscus and not the top. To ensure that quantities of the various chemicals are measured accurately, I have used the smallest pipettes or measuring cylinders for each quantity. For example, to measure 5ml of trypsin, I used a 5ml pipette. In this way, more accurate measurements can be made since more detailed gradations are available on the apparatus. Safety precautions that have been taken are the wearing of goggles throughout the experiment to avoid chemicals getting into the eye. Fair Test: To keep this investigation a fair test I will make sure that I will always measure out exactly 5ml of each substance before I mix them. ...read more.

Conclusion

For example, firstly, I could test the effect of concentration on the rate of reaction on other enzymes too, whose results could be used to back or test the conclusion found in this investigation. One example of this kind of experiment would be the reaction between hydrogen peroxide and liquidated celery. It could also have been hydrogen peroxide on a liver, but this reaction much too quickly to be able to record the results with any accuracy. This is because the liver has too many catalase enzymes, so the reactions are made a lot quicker making the investigation unfair. The experiment will be conducted as follows: Apparatus: * water basin * conical flask * bung, * delivery tube * measuring cylinder * syringe * spatula * stopwatch * electric scales (for increased accuracy) I will be timing the amount of oxygen that passes through the delivery time in a certain amount of time, after mixing the celery and Hydrogen Peroxide. I will take measurements from the side of the measuring cylinder every 30 seconds and note them down. From my results I would have found that the higher the concentration of hydrogen peroxide, the quicker the reaction rates producing oxygen. Suraj P. Nakum 10I GCSE Science Coursework Page 1/6 ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Patterns of Behaviour section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Patterns of Behaviour essays

  1. Find out how the rate of hydrolysis of an organic halogen compound depends on ...

    Medical attention should also be sought. If any vapours of the above substances are inhaled the victim must be removed to fresh air to rest, and kept warm. If this is not the case and it is more than a sniff, then medical attention should be sought.

  2. Investigating the effects of temperature on the rate of clotting milk and Rennet

    to draw up a results table clearly showing the results of the original tests and their repeats. I will then draw a line graph to show the relationship between the temperature and the time taken to react. It will also show which temperature the enzyme worked best in and gave the quickest reaction.

  1. The Effect of Catalase in the Breakdown of Hydrogen Peroxide

    However, the graph explains how each reading increases in time to the volume of oxygen. Nevertheless; many variables may have influenced my recording of that data. However; the data recorded seems to lie between each other so therefore; it enforces my hypothesis.

  2. Investigation On The Enzyme Trypsin

    238 242 240 240 6 54 55 54 54 7 43 44 45 44 8 57 57 59 58 9 62 62 61 62 10 71 73 72 72 As you can see my predictions were correct, increasing either the temperature or the concentration resulted in speeding up the reaction, and the optimum pH level seems to be around 7.

  1. Catalase activity in the liver

    so it wont be able to go any faster unless you change different factors (these are temperature, PH and concentration of substrate). I have also done some research to find out how this reaction happens and this is what I found that there is an Ion in the centre of

  2. Effect Of Substrate Concentration On The Activity Of Catalase

    all the three steps in the same way for all the experiments it should not make any difference to the overall result. b) It is also impossible to precisely measure out the amounts of Hydrogen Peroxide, Yeast and Water each time.

  1. Investigation into the digestion of milk by Trypsin.

    in the rate of reaction dropping to nothing as the temperature continues to rise past the enzymes point of denaturisation. I believe that similarly the rate of reaction will be proportional to the pH level until the pH rises past the optimum value of the enzyme.

  2. All roads lead to Rome? The multiple pathways of protein folding

    by either of two His residues, to the coordination position occupied by Met80 in the native protein. When this ligation is prevented by lowering the pH, 50-70% of the molecules fold rapidly (15 msec) with no observable intermediates. Under mildly denaturing conditions, cyt c forms a stable, compact intermediate which

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work