• Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

Stomata Investigation

Extracts from this document...

Introduction

Stomata Investigation The following experiment included an investigation into how the location of a leaf from a specific plant may effect its density of stomata on the lower epidermis. Stomata are one of the internal factors, which affect the rate of transpiration. It was hypothesised that leaves on the outer edge of a plant or tree would have a higher density of stomata due to environmental factors such as availability of light and air movement. The leaves used for the experiment were taken from a Holly tree, biologically referred to as Ilex. Materials: * Ilex- taken for experimental purposes ( 5 exterior and 5 interior) * Forceps- to remove impression * 10 Microscope slides and cover slips- for viewing under microscope * Video Microscope monitor- to observe and count stomata * Labels- for identification * Graph paper- outline drawing of leaf samples * Stage micrometer- measure the field view through microscope eyepiece * Pencil, Calculator, Ruler Method: 1. Materials are laid out on a single table for the easy access 2. Leaf samples are taken from the Ilex tree. 5 leave's taken from the outer edge and 5 from the plant inner part of the tree. ...read more.

Middle

But these environmental factors can also increase the rate of transpiration through the leaf by the stomata. Such leaves would possibly have a lower density of stomata in order to reduce the effect of transpiration. Just as external factors have an effect so do internal factors like a high density of stomata in a leaf, increasing transpiration. This questions whether the interior samples which seem to have a high number of distributed stomata, encounter a greater rate of transpiration? The biological significance of the Ilex leaf can explain some of the experimental results obtained. The Ilex plant is one of many plants living in dry conditions that show adaptations able to reduce the rate of transpiration. Such plants are referred to as Xerophytes and their adaptations as Xermorphic. The Ilex is a common evergreen shrub that grows below tree level- tolerant to dim light and the shade. This factor may explain why the interior samples taken from the shady bottom of the tree had a high stomata density and more stomata than the exterior samples tested. It is able to grow in woodland conditions all year round, even in winter months (when it was taken for experiment purposes). ...read more.

Conclusion

The magnification used was at (x10) making the vision big for better clarity but it also meant only a small section had been viewed. If the magnification were to be larger, stomata could be observed more from a larger field view and results would both be more reliable and accurate. Another method that involves impressions of a leaf epidermis could be made using a dental-silicone impression material such as, Provil. Instead of applying nail varnish directly onto the leaf. The base and catalyst are mixed together in equal proportions then spread onto the leaf samples. The material sets rapidly and after removal can be used to prepare positive impressions using clear nail varnish. To further the investigation leaves of the Ilex tree could be taken from various growing areas and tested to see if the same hypothesis applies. Leaves could be taken according to seasons e.g. Spring- when Ilex flowers begin to bloom, and see whether seasonal changes have an influence on stomata density for both inner and outer edged leaves. The use of another plant such as the Sugar Maple tree can be investigated using a similar hypothesis and experimental method as suggested above. Or alternatively the results of the Sugar Maple could be compared to those of the Ilex tree. ...read more.

The above preview is unformatted text

This student written piece of work is one of many that can be found in our GCSE Green Plants as Organisms section.

Found what you're looking for?

  • Start learning 29% faster today
  • 150,000+ documents available
  • Just £6.99 a month

Not the one? Search for your essay title...
  • Join over 1.2 million students every month
  • Accelerate your learning by 29%
  • Unlimited access from just £6.99 per month

See related essaysSee related essays

Related GCSE Green Plants as Organisms essays

  1. Photosynthesis Investigation

    (This can also been seen in the result diagram above.) Testing for Water Aim My aim for this experiment is whether water is necessary/unnecessary for a plant to photosynthesize Prediction I can predict water is as necessary as all others I investigated; this is because plants cannot survive without water as they can't make their own food.

  2. The location of panama

    (See fig...next page) How the Canal works The Panama Canal extends approximately 50 miles from Panama City on the Pacific Ocean to the Caribbean Sea. To travel from the Atlantic to the pacific a ship enters a 7 mile dredged channel in Limon Bay.

  1. Construct a rocket from a discarded PET (Polthylene Teraphetlate) drink bottle.

    In the above diagram we have illustrated what the setup should look like. The bricks should always be titled at a 45-degree angle; we kept a person to make sure there were no errors in this. 2) After each launch we made sure that the rocket was not damaged or

  2. An Investigation into Species Diversity with distance along a Pingo.

    A table showing the precautions that must be taken in the lab Risk Treatment Precaution Spillage of liquids when testing the pH of the soil samples Wipe the surface with a damp cloth to remove any stains that the universal indicator may leave.

  1. Find out where the stomata are located, on the upper or lower epidermis of ...

    So we must look for an alternative explanation. One possible hypothesis depends on the fact that the enzymatic conversion of starch to sugar proceeds more readily when comparatively little acid is present (i.e., at a high pH). The conversion to sugar to starch on the other hand is favored by a comparatively high concentration of acid (low pH).

  2. The aim of this science investigation is to find out where the stomata are ...

    So we must look for an alternative explanation. One possible hypothesis depends on the fact that the enzymatic conversion of starch to sugar proceeds more readily when comparatively little acid is present (i.e., at a high pH). The conversion to sugar to starch on the other hand is favored by a comparatively high concentration of acid (low pH).

  1. Investigating thegrowth of pleurococcus on tree trunks

    (O-E) � ? E = ? (103 - 72)( ? 72 X = 13.34 South X = ? (O-E) � ? E = ? (63-72)( ? 72 X = 1.125 East X = ? (O-E) � ? E = ? (74-72)( ? 72 X = 0.05 West X = ? (O-E) � ? E = ?

  2. The investigation is aiming to look at transpiration.

    The attraction is called cohesion. They are also attracted to the lignin in the walls of the xylem vessels; this attraction is called adhesion. Cohesion and adhesion help to keep water in a xylem vessel moving in a continuous column.

  • Over 160,000 pieces
    of student written work
  • Annotated by
    experienced teachers
  • Ideas and feedback to
    improve your own work