Testing For Non-Reducing Sugars

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Testing For Non-Reducing Sugars

Aim: To test for non-reducing sugars 8 solutions of Glucose and compare their colour change compared with 3 different types of everyday liquids that contain sugar content.

Equipment: The Equipment that will be needed for this experiment is:

* Spatula

* A sample to test: the sucrose solution

* Test tube holder

* 1 mol per dm hydrochloric acid, sodium hydrogen carbonate

* Benedict's solution

* Pipette

* Water Bath tank

* Eye protection

Method: For this experiment I am going to:

* Measure 5cm of Sucrose Solution into a boiling tube and add 1cm of dilute Hydrochloric Acid (HCl)

* Stand the Tubes in a Boiling Water Bath by using a rack and leave for 5 Minutes. Remove the Rack with all the Test Tubes and leave to cool for a short period of time.

* Carefully add Sodium Hydrogen carbonate to the solution in the tubes, this should cause a reaction to occur where the solution fizzes. Add small amounts of Sodium Hydrogen carbonate until the fizzing eventually stops.

* Now add