The aim is to find out how osmosis is affected by different concentrations of salt solution, in potato tissue.

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AIM: the aim is to find out how osmosis is affected by different concentrations of salt solution, in potato tissue.

Diagram:

                       Bung      salt solution       test tube              Potato chip

        Distilled            0.25%                 0.5%                0.75%           1.0%        

Concentrations of salt solution

Safety:

  • Tie your hair back – because loose hair can interfere with practical work.  It might dip into acid etc.
  • No running- because this will cause lots of accidents, there is more chance of slipping and you may bump into other people.
  • Clear up any spillages – because other people can slip and some substances can be harmful.
  • Stand up – so that if there is a spillage or explosion it is easier for you to get away.
  • Tuck stools under tables – so that people don’t bump into them.

Equipment:

  • Test tubes X 15                bungs X 15
  • Borer                                measuring cylinder
  • Test tubes racks                tile
  • Ruler                                scales
  • Knife                                tissue
  • Plain sticky labels        potato
  • Five different concentrations of salt solution.

The input variable for this investigation was the different concentrations of salt solution.  Each time the salt solution will get more concentrated by 0.25%.

The output variable in this investigation was the weight of the potato.  The potato was weighed before and after the experiment to see if it had gained or lost weight.

The constant variables were the length and width of the potato, the volume of salt solution (15cm3).  Also the same potato was used for all 15 chips and the chips were left in the solution for the same time.

The Range of salt solutions was from distilled water to 1% salt solution.  (Distilled, 0.25%, 0.50%, 0.75%, 1.0%)

Each concentration was repeated three times for accuracy.  There were three potato chips in each concentration of salt solution.

Method

  • First of all using a borer, which had a diameter of 5mm, 15 chips of potato were extracted from the same potato.
  • Then each chip was measured to a length of 3 cm and cut so that there were 15 identical chips.
  • Every single chip was then wiped (rolled on paper) slightly to take off any excess water and weighed.
  • The individual weights were then recorded.
  • Next, 15 test tubes were put into test tube racks and labelled with the salt concentration that they would contain and the weight of the chip that it contained.
  • Then we chose which five concentrations of salt solution we wanted to use.  I chose distilled to 1.0% because then I have a consecutive range of solution and this would consequently make it easier for me to analyse the results.
  •  15cm3   of each concentration was then measured and put into three test tubes.
  • The chips were put into the test tubes and the test tubes were sealed using the bungs.
  • The entire set up was then left for around 26 hours.
  • Then the chips were removed from the salt solutions and wiped again slightly to remove any excess solution.
  • They were then weighed and the results were recorded.
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Prediction with reason

        I predict that the mass will increase for the lower concentrations and decrease for the higher concentrations.  The reason I predict this is because of my knowledge of osmosis.  Osmosis is a special case of diffusion.  Osmosis is the movement of water particles through a semi- permeable membrane.  The water diffuses across the permeable membrane from where there is a high water potential to where there is a low water potential.  (They go from where there are lots of water particles to where there are less water particles.)  The diagram below shows this information:

         

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